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10 jars were inoculated with 1cc Gulf Coast spores each. The substrate was sterilized brown rice flour and water with wet sterilized vermiculite. The jars were 1/2 pint with substrate material and a dry vermiculite layer on top. The water used was tap water for most, but a few (don't know how many or which) had boiled water. The jars were sterilized with the lids off in a microwave and then the unsterilized lids were put on with unsterilized tape over the 4 holes in each. The tape was left off one jar.
Now it has been 12 days and all jars have at least some mycelium growth. The one without tape has the most and 3 other jars look fine, but the other 6 jars have a brown color on the inside of some of the tape and most of these have little or no mycelium growth under the spot with the brown.
I am asking what should be done, if anything can be, and if anyone has seen this before what happened for you.
it is burned substrate. microwave sterilization isnt the best technique. it heats some areas til they burn and others relatively none at all. The reason there is no mycelium in the area under the spot with the brown is that it has been burned, and therefore lost nearly all moisture, and it will never be possible for mycelium to colonize these areas. These areas are probably all over througout your jars. I would recommend boiling for sterilization next time. I'm sure you have all you need : a pot, a lid, and a stove. a friend of mine uses only steam sterilization (75 min.), no pressure cooker just a pot and lid, and has had a 100% success rate on 30 jars. Good luck with whatever you choose to do.
-------------------- ^ Above post is complete fiction. ^