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OfflineTheDoc
newbie
Registered: 08/10/03
Posts: 25
Loc: Tahawus, NY
Last seen: 12 years, 10 months
Massive contams with Koh Sam SS
    #2082266 - 11/07/03 10:32 PM (13 years, 29 days ago)

The following describes events in a long running dream I seem to be trapped in:

Yikes! After four days in the jar my KS-SS on wheat is showing MASSIVE contamination. Like worse that I've ever seen. Basic outline follows.

Each quart jar got 3/4 cup of wheat and 3/4 cup of water. Then they sat for 24 hours before being stirred and topped with lids prepped with pollyfill and sealed inoculation holes. PC'd at 15psi for an hour, (A full hour AT 15psi) cooled and then knocked up with about 1.2cc per jar. No signs at 48 hours at 80F, total contam wasteland at 96 hours.

Due to a limited spore supply I'm going to try the Karo water tek next. I'm also going to soak the grain for 12-16 hours and then rinse it before canning and adding water to correct the moisture level and PCing it.

I used pretty good (worked fine in the past) sterile techniques, gloves, disinfectant spray, oven tek ect. But can anyone see any obvious cause for my contam problems that I'm missing here?

Thanks,
Doc


--------------------
I'm a liar. All posts are thoeretical only. If I claim to have grown or be in the process of growing and mushroom other than legal edible mushrooms I'm full of uncomposted crap or am a fed sitting in the cubical next to yours... Or maybe I'm undercover, so don't blow my cover. Go away.


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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Massive contams with Koh Sam SS [Re: TheDoc]
    #2082315 - 11/07/03 11:02 PM (13 years, 29 days ago)

It's not the strain, it's your procedure. Better luck next time.


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OfflineTheDoc
newbie
Registered: 08/10/03
Posts: 25
Loc: Tahawus, NY
Last seen: 12 years, 10 months
Re: Massive contams with Koh Sam SS [Re: SixCee]
    #2082661 - 11/08/03 01:06 AM (13 years, 29 days ago)

Well I know its not the strain, but it may have been the syringe or something in my procedure. I was hoping that something in my tek would jump out at someone as a problem.

Thanks,
Doc


--------------------
I'm a liar. All posts are thoeretical only. If I claim to have grown or be in the process of growing and mushroom other than legal edible mushrooms I'm full of uncomposted crap or am a fed sitting in the cubical next to yours... Or maybe I'm undercover, so don't blow my cover. Go away.


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OfflineBen_Stein
Spelling Gestapo

Registered: 08/16/03
Posts: 370
Loc: Hooterville
Last seen: 12 years, 3 months
Re: Massive contams with Koh Sam SS [Re: TheDoc]
    #2082676 - 11/08/03 01:11 AM (13 years, 29 days ago)

Try preparing a few jars and waiting 4 or 5 days without inoculating.

This should at least tell you if your initial sterilization was sufficient.


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


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OfflineTheDoc
newbie
Registered: 08/10/03
Posts: 25
Loc: Tahawus, NY
Last seen: 12 years, 10 months
Re: Massive contams with Koh Sam SS [Re: Ben_Stein]
    #2082714 - 11/08/03 01:19 AM (13 years, 29 days ago)

Oh yea, I'm using soft wheat, I can't find hard or rye grain.

Doc


--------------------
I'm a liar. All posts are thoeretical only. If I claim to have grown or be in the process of growing and mushroom other than legal edible mushrooms I'm full of uncomposted crap or am a fed sitting in the cubical next to yours... Or maybe I'm undercover, so don't blow my cover. Go away.


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InvisibleHanky
wiffle bat.
Male User Gallery
Registered: 08/30/03
Posts: 56,993
Loc: Great Southern Land.
Re: Massive contams with Koh Sam SS [Re: TheDoc]
    #2082729 - 11/08/03 01:25 AM (13 years, 29 days ago)

One vector you could maybe point the finger at is using the oven tek.
personaly i belive that using oven doors as a clean work area is at best a game of contam roulette.
Consider building a glovebox or a flowhood.


--------------------
Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]




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OfflineBen_Stein
Spelling Gestapo

Registered: 08/16/03
Posts: 370
Loc: Hooterville
Last seen: 12 years, 3 months
Re: Massive contams with Koh Sam SS [Re: Hanky]
    #2083311 - 11/08/03 08:37 AM (13 years, 28 days ago)

^^^^^Ya, What he said^^^^^
Right on, Hanky!

I forgot to mention my  opinion on that: (For what it's worth)I think the so-called "Oven Tek" blows.  NEVER worked for me.

While a glovebox or flowhood would be preferred, I have the adopted the smallest room in my crib, a spare bathroom, as my lab.  By no means the cleanest room, but easily sterilized in just a few minutes.

Since making the switch I'm batting 1000.  I've pulled off some interesting stunts in there and believe I could safely do G2G transfers as well.

Of course, there's something fringing on unholy when a scientist over eats and has to take a dump in his sacred lab.

sorry, ....but it always go through my mind.  :rolleyes:


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


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Amazon Shop for: ½ Pint Jars, Rye Grain

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