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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Inocuole]
#20801458 - 11/06/14 01:36 PM (9 years, 3 months ago) |
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You dip it into the non-colonized portion of agar before you cut the mycelium out. It's basically like when people spray out a bit of boiled liquid in the tip of a syringe to have the fresh solution cool off the tip.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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propane torch ftw red hot in seconds I wrappped the end of the handle in a paper towel and wrapped that in masking tape. Now i never feel the heat.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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I'll definitely have to add something to it, probably just the very end in foil.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Too cool the blade you should dip it into the reciving plate to cool. This will prevent cross contamination. Cron has a plate that he designates his cooling plate and just does not transfer anything to it.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Quote:
tombosley8 said: propane torch ftw red hot in seconds I wrappped the end of the handle in a paper towel and wrapped that in masking tape. Now i never feel the heat.
I use a blow torch for sterilizing needles/scalpels and it works great...few seconds and it's glowing.
I've seen people say this would disrupt the still air in the room/create currents, which it probably does, but it's never been an issue at all. Just don't have it in your SAB
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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An actual blow torch? That seems like overkill, heh.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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idk a propane torch works great. it's outside of the SAB anyway so the air current thing is moot
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Yeah the still air thing, eh whatever. Here's what I use. Have to turn it way down though, thing is powerful.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I have used a propane torch for a while and love it. I ran out of propane last week though and instead of buying a new canister I picked up a smaller butane torch for 20 dollars. It works the same but is a lot smaller and the flame doesn't get my sterile work tent quite as hot. Don't get a torch at a head shop. Go to a hardware store. They are way cheaper there.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Re: Pasty Agar Tek [Re: MudaFuka]
#20805140 - 11/07/14 10:14 AM (9 years, 3 months ago) |
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I use my gas stove, heat the scalpel then I put it in a bowl of alcohol, heat, use, alcohol. Heat, use, alcohol.
No contam yet, but could be dumb luck.
Also, I've got two of my 3rd transfers showing really ropey growth -- super stoked. Going to cook up some agar now for my 4th transfer plates.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Pudgy]
#20805162 - 11/07/14 10:24 AM (9 years, 3 months ago) |
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Don't put your instruments in alcohol after flame sterilizing. That's backwards. Once you flame sterilize, your injection or slicing point shouldn't hit alcohol as it just reintroduces your instrument to shitty conditions.
Flame and you're good.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Ah I see. Noted.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Pudgy]
#20805263 - 11/07/14 10:49 AM (9 years, 3 months ago) |
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Yup! Heat kills EVERYTHING, alcohol doesn't, so it makes no sense to do both, especially alcohol last. Although I will wipe down instruments with alcohol with parts that can't be flame sterilized, e.g., scalpel handle, syringe plunger, etc.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Updated pictures of some of the last plates:
1) Is this a prime candidate for transferring since it's so scattered in development?

2) Thoughts on this one and it's second cluster of mycelium? I planned on making some more inoculant and LC soon, was thinking of these guys?

3) This is the plate with the mycelium growing on one side, partially on the rim of the plate. I can't tell if this bacteria or just the funny angle of the red agar being eaten away and taken over by white mycelium. The top of it is only white.

Appreciate thoughts on any and all cultures! Thanks!
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 21 hours, 39 minutes
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Quote:
tripdawg420 said:

LOL WTF?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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That mycelium, if it is mushroom mycelium, looks weird as fuck.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#20809907 - 11/08/14 12:41 PM (9 years, 3 months ago) |
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The satellite is a contam. The others look like they are chock full of genetics, transfer them and see what you get. Might be some ride alongs in there so watch the recovery close.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Inocuole]
#20810001 - 11/08/14 01:24 PM (9 years, 3 months ago) |
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Quote:
Inocuole said: That mycelium, if it is mushroom mycelium, looks weird as fuck.
Everyone? Can you please be specific as to what makes it weird?
Pasty,
Are satellites nearly always bacteria or is that one special? I dropped the first plate into 2 LC's just a minute ago, but I'll transfer out the satellite dish (hehe) into new agar when I have some.
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