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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
stareatclouds said: I'm wary about removing the paper towel and infecting it since I don't have any micropore tape yet. Also the paper towels stick to the tape I use and cover the biggest portion of the lid. I'll try in my SAB next time though. You using a camera phone?
Here's a (rather large, sorry) GIF of me dropping an agar wedge into the distilled water thing for your LI Tek. I have a full video, but I think this is the most crucial part. Please critique!
http://i.imgur.com/yM3zq9M.gifv
By the way, an X-acto knife SUCKS for this. When you heat up the blade, it heats up the entire handle and is soo f'ing hot. Do scalpels do this?
My handle doesn't get hot when I do this? Weird. One thing you want to do is not have the agar chunk floating in midair so long with the plate wide open. That was like 15-20 seconds it looked like of possible bacterial free-for-all on that plate and your transferred chunk. You want that jar unscrewed and the seal on the lid undone so that it's just resting on top beforehand, so when you make the transfer so you can only have each lid open for as long as it takes to get the inoculant in/out with no lingering. It should take you less than 2-3 seconds to get it from the first container to the second. I always slice my agar wedges in little triangles, it seems to be the easiest way to get a workable piece out.
Now I kinda want to post a gif/video of my technique so I can see what I'm doing wrong. I did some transfers today after taking some extra wiping down with alcohol precautions, I guess we'll see how that works in a few days.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Quote:
wowimflabbergasted said: How many hours a day do you put towards this hobby dude?
A day? On avg.? ......... .25
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Quote:
PussyFart said:
Quote:
wowimflabbergasted said: How many hours a day do you put towards this hobby dude?
A day? On avg.? ......... .25
what about how much time do you put towards the shroomery
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Inocuole]
#20799127 - 11/05/14 09:32 PM (9 years, 3 months ago) |
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Quote:
Inocuole said: My handle doesn't get hot when I do this? Weird.
Are you using a scalpel or an X-acto blade?
Quote:
One thing you want to do is not have the agar chunk floating in midair so long with the plate wide open. That was like 15-20 seconds it looked like of possible bacterial free-for-all on that plate and your transferred chunk. You want that jar unscrewed and the seal on the lid undone so that it's just resting on top beforehand, so when you make the transfer so you can only have each lid open for as long as it takes to get the inoculant in/out with no lingering. It should take you less than 2-3 seconds to get it from the first container to the second. I always slice my agar wedges in little triangles, it seems to be the easiest way to get a workable piece out.
Hmm, I'll have to work on that. I guess I could take the ring completely off so I only have to lift up the blender piece and not fiddle with both. I'll probably cut the piece out and move the whole cup sideways to the top of the receiving jar next time. That should provide additional cover.
Quote:
Now I kinda want to post a gif/video of my technique so I can see what I'm doing wrong. I did some transfers today after taking some extra wiping down with alcohol precautions, I guess we'll see how that works in a few days.
Do it!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I recently silicone the blade attachment to a jar ring so it all comes off in one piece.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: MudaFuka]
#20799180 - 11/05/14 09:46 PM (9 years, 3 months ago) |
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I was actually thinking about doing that, but wanted to run it by you guys first. Was it as easy as it would seem?
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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 yup
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: MudaFuka]
#20799206 - 11/05/14 09:52 PM (9 years, 3 months ago) |
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Cool, I'll do that soon. Your OP says Bathroom/Kitchen Silicone is better than RTV, but later you and eat said RTV is fine. Either or?
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I do prefer kitchen and bath or exterior silicone but RVT dose work.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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Re: Pasty Agar Tek [Re: MudaFuka]
#20799735 - 11/06/14 12:26 AM (9 years, 3 months ago) |
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Hey now what's with all the thread jacking?
so here ready for the first tiger drop with my wider mouth ziploc rounds and just wanted to show it off. It was an accident originally when i overheated my lid while torching it to attach stiff felt via dilated's no silicon lid tek and ended up with a wide open hole that would just fit the blade through. SO i decided it was destiny that i figure out how to use it with the oster blenders. THEN I meticulously started loosening screws on the blender base and soon got fed up with that and took a pair of some giant heavy duty pliers and went at it. I just broke the plastic rims off from above the blending rod on the base and it works great. THEEEEEN I realized i could have just used a drill to blend the jar. OH WELLLL. Then i siliconed the blade attachment to the lid on both sides  for real SORRY for the thread jack The plastic lids slightly leak SO THE ABOVE METHOD SHOULD BE REFRAINED FROM. INSTEAD TRY THIS!!!:Quote:
tombosley8 said: THIS JUST IN FOLKs:!!!!
    Studies have found that there should be a mass recall on all blender attachment conversions on widemouth plastic lids (what i posted earlier ) IT is apparent that there is a slow drip that could be detrimental to the health of our babies across the world. The Solution was revealed earlier today by a team of top researchers. They said not to be in fear as the metal lids do in fact create a safe seal and that any further commencement involving altering lids in this way should be done in a metal fashion. That concludes that our pesky and threatening drip is no longer at large and we may all return safely to our residences and be assured it will all be ok.
I will be using my pasty plates to inoculate MUDUFUKA's LI and bottles via the Tiger Drop. Thank you all OH yeah i left out eatyoualives's slurry, FOO MAN's WBS, and spitball jedi's rye will be in the mix PROPS
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Edited by tombosley8 (11/09/14 02:07 PM)
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Oh shit I didn't eaven notice what thread this was I was just replying without paying too much atention. Sorry pasty.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 12 days
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Re: Pasty Agar Tek [Re: MudaFuka]
#20800543 - 11/06/14 09:07 AM (9 years, 3 months ago) |
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Ha, that did make me laugh. You, Pasty and Blinding are just like Shroomy best mates anyway, im sure it will be fine. The SBM brothers.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: Edmunter]
#20800997 - 11/06/14 11:39 AM (9 years, 3 months ago) |
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@stare yeah it's an x-acto. It's got a metal handle like yours too. I hold mine pretty far from the end where I heat it, and my gloves are kind of thick too, so I may just not be feeling it, but how long are you holding the blade over a flame? It shouldn't take more than 5-10 seconds usually?
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#20801018 - 11/06/14 11:45 AM (9 years, 3 months ago) |
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I find when I am doing 20 or 30 transfers at a time the handle gets crazy hot. It is great motivation to work quickly though.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: MudaFuka]
#20801037 - 11/06/14 11:50 AM (9 years, 3 months ago) |
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wrap some foil around the handle!!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: MudaFuka]
#20801039 - 11/06/14 11:51 AM (9 years, 3 months ago) |
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Oh, I hadn't really thought of that. I've only been doing a few transfers at a time. I bet if you use a torch it gets hot as fuck too. I'm thinking about getting a small torch instead of going the alcohol lamp route just because of general availability.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: Edmunter]
#20801046 - 11/06/14 11:53 AM (9 years, 3 months ago) |
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Quote:
Edmunter said: Ha, that did make me laugh. You, Pasty and Blinding are just like Shroomy best mates anyway, im sure it will be fine. The SBM brothers.
we were trying to get married, but shroomery has not legalized gay marriage yet.
innocuole, i think u will like the torch more. i only use my alcohol lamp when my torch runs out of gas. the torch makes ur blade red hot in 5 second flat.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Sounds good, may hit up the head shop for one soon. If and when I write up my own "how I do shit" I'll probably include that. It's a cheap non-intimidating alternative to using a wick and some solvent in a jar, plus it seems more effective, and reduces air currents around your box.
An alcohol lamp to me, just seems like one fumble from a molotov cocktail.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Inocuole]
#20801307 - 11/06/14 12:59 PM (9 years, 3 months ago) |
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Quote:
Inocuole said: @stare yeah it's an x-acto. It's got a metal handle like yours too. I hold mine pretty far from the end where I heat it, and my gloves are kind of thick too, so I may just not be feeling it, but how long are you holding the blade over a flame? It shouldn't take more than 5-10 seconds usually?
I waited until the blade itself got red hot which seemed to have taken like 30 seconds or so.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
stareatclouds said:
Quote:
Inocuole said: @stare yeah it's an x-acto. It's got a metal handle like yours too. I hold mine pretty far from the end where I heat it, and my gloves are kind of thick too, so I may just not be feeling it, but how long are you holding the blade over a flame? It shouldn't take more than 5-10 seconds usually?
I waited until the blade itself got red hot which seemed to have taken like 30 seconds or so.
Makes sense. Maybe I'm doing it more wrong than you are. I couldn't get my blade red hot so I gave up on it, and just held it there for about as long as I normally did my other tools that did glow red. I only ever use the very tip of the blade. Once I get a torch I suspect that'll improve things.
What the hell do I do with a red hot blade exactly to cool it off, or do I just have to cauterize the shit out of everything I touch with it?
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