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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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How many do you want? Take a couple chose the best of those and nock up grains 
Or keep transferring and get a bunch of isolates. All depends what you want to do.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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I guess I'm confused on the terminology. I thought I was supposed to transfer to keep it growing and I'd isolate the best performing chunk of the mycelium? Or is that not correct?
I plan on inoculating some grains soon, probably WBS since I hear that's kind of easy to come by, so I'll probably want enough for a few jars of that.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 12 days
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Quote:
stareatclouds said: I guess I'm confused on the terminology. I thought I was supposed to transfer to keep it growing and I'd isolate the best performing chunk of the mycelium? Or is that not correct?
I plan on inoculating some grains soon, probably WBS since I hear that's kind of easy to come by, so I'll probably want enough for a few jars of that.
I love this guys write up.. might help you http://www.shroomery.org/forums/showflat.php/Number/18430998
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Edmunter]
#20784629 - 11/02/14 03:46 PM (9 years, 3 months ago) |
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Yeah, I think you showed me that earlier in this thread and it's an awesome write up. I guess if my culture is clean already, I can just transfer it so I have more and not need to isolate it from any contams. I'll let it grow out a bit more.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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For what it's worth, I am on my third transfers. When I did my transfers I inoculated 8 grain jars and this experimental grow Violet is hosting.
I took the sexiest bit of mycelium from my sites and put them on new agar, the result is not only what I expected but pleasing.
The rest of the myc sites I didn't want to transfer, went to grain. The result was a ton of healthy nice mycelium in some sterilized sub. So it's unbelievably beneficial, especially to the newb.
I still feel grateful to Pasty and MudaFuka when I check on my fellas daily.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: Pudgy]
#20787903 - 11/03/14 12:23 PM (9 years, 3 months ago) |
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Yeah I like how even though I didn't do everything I was supposed to do, I still have a perfect clean plate and some others that can still be transferred from. I also just shook my first agar->grain jar and it's recovering really well. That means I overcame my original vector that was making my grows hard.
This tek in particular is the knees of the mo'fuckin bees.
Sure I've got some bad plates, some don't even have mycelium, just bacteria. But the difference between agar and just about every other method of growing is that you can see EXACTLY where you fucked up because nothing can jump in 3d in there. It makes it so hard to fuck shit up irreversibly.
Also the food coloring really helps, I wouldn't skip that step for anything.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Inocuole]
#20787923 - 11/03/14 12:28 PM (9 years, 3 months ago) |
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Yeah mang, I never thought I'd be cool with seeing bacteria, but seeing as how it was basically like a $.02 mixture in a reusable plastic cup, it's no big deal at all.
I'm pretty sure I have a definite clean culture and 2 other possible ones. One of which is all the way on the left and climbing the wall of the cup, so maybe not, but we'll see.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Got some more pics of various plates with those bacteria spots and some clean ones as well. Taking pictures through the lid is pretty damn easy when it gets condensation on the sides.
On this first one, should I try to transfer that whole cluster right there next to the fatty? If I could get a whole sub surface to cluster like that little spot, good lord.





You can totally see why some of those plates contamed. I sprayed a certain batch of them with lysol accidentally and I couldn't get all the shit off of them and, with moving and the cat hair everywhere... yeah.. bad times. I have a special no cat-zone room for all this now, and am much cleaner going forward.
This last one is an agar to coir experiment I did on a plate I had already transferred from. Basically just prepared a small amount of coir (didn't even pasteurize fully, or bucket tek, just hot water.) dropped right on top of the colonized agar. It's pinning, but, the agar was preparing to anyway. Not sure that much will come from it at all, just wanted to see how quickly it would grow through such a thin layer of coir since the only other thing I could think of was to toss the plate.

It was cool watching it grow up the sides. It did so with a ferocity.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 12 days
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Re: Pasty Agar Tek [Re: Inocuole]
#20788218 - 11/03/14 01:42 PM (9 years, 3 months ago) |
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Here my next set of transfers. Im getting really close now.
I used a small amount of agar mix which has made the myc thin. Should I transfer again to something deeper in a few days?
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lbigelow
Stranger



Registered: 07/16/14
Posts: 50
Last seen: 8 years, 7 months
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Re: Pasty Agar Tek [Re: Edmunter]
#20789672 - 11/03/14 07:08 PM (9 years, 3 months ago) |
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I was under the impression that the myc just grew on top of the agar. Does that make me sound stupid?
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lbigelow
Stranger



Registered: 07/16/14
Posts: 50
Last seen: 8 years, 7 months
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Re: Pasty Agar Tek [Re: lbigelow]
#20789681 - 11/03/14 07:09 PM (9 years, 3 months ago) |
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Does the agar depth matter?
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: lbigelow]
#20789818 - 11/03/14 07:31 PM (9 years, 3 months ago) |
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Deeper agar is better for storage. Thinner plates dry out faster.
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lbigelow
Stranger



Registered: 07/16/14
Posts: 50
Last seen: 8 years, 7 months
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Re: Pasty Agar Tek [Re: MudaFuka]
#20789926 - 11/03/14 07:56 PM (9 years, 3 months ago) |
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Ah. Thank you, sir.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#20790014 - 11/03/14 08:20 PM (9 years, 3 months ago) |
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Quote:
Inocuole said: On this first one, should I try to transfer that whole cluster right there next to the fatty? If I could get a whole sub surface to cluster like that little spot, good lord.

It would probably be the shit, do it 
I never took a pic of this one in a tub but its the tits, easily 110% BE first flush. I really need to grow it again, its been over a year. Its pinned in every plate I ever put it in.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Is likelihood to pin on a plate correlated at all with its likelihood to pin on CVG and rye? Or can that be hit or miss too?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#20790493 - 11/03/14 10:28 PM (9 years, 3 months ago) |
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Usually pins on a plate mean strong fruiting genetics. I mean a plate has no fae, barely any water or nutes, when its gets to a mono its like
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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If the mycelium pinning in agar is such a strong indicator of fruiting genetics, why don't we always wait and see if it pins or not? I see people cutting up the mycelium and transferring it very early most of the time.
MAKE ME UNDERSTAND
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Sorry for the shitty pictures, but snapping shots through the plastic is impossible.
These are the same cup (the one I posted a bit ago). It still looks clean so I should probably transfer some chunks on the end out to new cups, yeah?

This is basically impossible to see, but the little bit of mycelium is on the side of the jar. I'm pretty sure this is a cup I dropped after dropping a bit of solution down. Just toss it or what?

There's 3 little colonies of mycelium, but all of them look clean so far. Thoughts?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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i'd say for the first one when ever in doubt just make the transfer for the second one there si no reason to toss till your sure...beauty of agar my friend third one i'd say transfer the fastest growth if it looks right
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Re: Pasty Agar Tek [Re: cronicr]
#20790813 - 11/04/14 12:20 AM (9 years, 3 months ago) |
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