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Offlinemushpunx
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How long can you keep sterile grain jars?
    #20781303 - 11/01/14 06:59 PM (10 years, 2 months ago)

Once they're PC ed what's the longest I should leave them around before I inoculate?

I mean Ideally id drop my wedge in right after they cool but if I prep too much rye and have extra jars what's the longest I should keep them for?

A re they clean to use u ntill the moisture content goes?


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Invisiblemaddchef
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Re: How long can you keep sterile grain jars? [Re: mushpunx]
    #20781313 - 11/01/14 07:01 PM (10 years, 2 months ago)

Generally if you prepped them right yes. I've used jars that were about a month old. They were a bit dry but otherwise fine.


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In the land of the blind, the one eyed man is king.

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OfflineTheMustardTiger
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Re: How long can you keep sterile grain jars? [Re: maddchef]
    #20781349 - 11/01/14 07:10 PM (10 years, 2 months ago)

Not saying you did this, but most people tend to have over saturated jars after the PC cycle and you'd be wise to have them dryer rather than wet. How long are you planning to leave them?


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Offlinemushpunx
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Re: How long can you keep sterile grain jars? [Re: TheMustardTiger]
    #20781433 - 11/01/14 07:35 PM (10 years, 2 months ago)

Thanks for the input!


I cooked these jars last night, I was lazy today and didnt feel like doing my G2Gs.
I could stretch my masters to do all the jars but Id rather save the extra jars till my clone masters are ready, maybe a week

But yea the question of shelf life came up and I figured I might as well ask so I know right


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OfflineTheMustardTiger
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Re: How long can you keep sterile grain jars? [Re: mushpunx]
    #20781434 - 11/01/14 07:36 PM (10 years, 2 months ago)

A week is probably fine. You're only concern should be surviving spores germinating. You want your mycelium to get a foothold before anything else.


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Edited by TheMustardTiger (11/01/14 07:37 PM)

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Offlinemushpunx
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Re: How long can you keep sterile grain jars? [Re: TheMustardTiger]
    #20781542 - 11/01/14 08:13 PM (10 years, 2 months ago)

Hah I just realized why your screen name means haha phil Collins


I mean now that I think about it mushroom sites sell grain jars it must take some time to prep amd send em yea?


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OfflineTheMustardTiger
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Re: How long can you keep sterile grain jars? [Re: mushpunx]
    #20781551 - 11/01/14 08:16 PM (10 years, 2 months ago)

Right, so a week should be fine. Just inoculate asap.


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OfflinehamloafM
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Re: How long can you keep sterile grain jars? [Re: TheMustardTiger]
    #20782149 - 11/01/14 11:28 PM (10 years, 2 months ago)

If those jars are properly sterilized, sealed and your lab's sterile equilibrium is maintained those jars should never contaminate.  Over time the sterlized grains will lose their moisture content though. 

It's always best to leave a few sterlized mediums in your lab uninoculated anyways.  This helps to show you where your vectors of contamination are open should you have any open.

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Offlineblackout
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Re: How long can you keep sterile grain jars? [Re: hamloaf]
    #20782645 - 11/02/14 02:48 AM (10 years, 2 months ago)

I have the weights of my jars including filters written on them all. On the jar I also write the weight of the finished jar with the grain in it.

This way if it dries out at all I know the original weight and can inject water to compensate. I am often adding large amounts of myc water so need to know moisture levels.

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Offlineethanstarkey
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Re: How long can you keep sterile grain jars? [Re: blackout]
    #27022311 - 11/04/20 08:41 PM (4 years, 2 months ago)

Oh boy, I didn't think about this and made a bunch at once and won't use them in time. Could I re-saturate the grain and re-sterilize them (or something like that)? If so what should I do exactly?

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OfflineInfinity-25
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Re: How long can you keep sterile grain jars? [Re: ethanstarkey]
    #27022558 - 11/05/20 04:00 AM (4 years, 2 months ago)

I first put my agar wedges in sterile water and shake the sh%t out of them. With this I inoculate my jars. If they are on the dry side, just pour in a bit more. Could be an option for you too. If the jars are proper sterile its shouldn't matter how long you wait then. I like this method as the colonisation of the jars is much faster than with intact agar wedges.

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