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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20969027 - 12/12/14 10:07 PM (10 years, 1 month ago) |
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Quote:
taGyo said: I recently started another BRF tek project because Munch encouraged me and I was thinking about using a tyvek filter instead of dry verm...

Then I just used the dry verm 
They're all LCs and doing well now
How hard is lc?
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 7 years, 5 months
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20969037 - 12/12/14 10:09 PM (10 years, 1 month ago) |
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Quote:
Cryptology said:
Quote:
taGyo said: I recently started another BRF tek project because Munch encouraged me and I was thinking about using a tyvek filter instead of dry verm...

Then I just used the dry verm 
They're all LCs and doing well now
How hard is lc?
Getting a clean LC might prove to be difficult for a noob but it's certainly not rocket science. Some people around here swear by them. You just need to be on your A game when it comes to sterility.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: bodhisatta]
#20969047 - 12/12/14 10:13 PM (10 years, 1 month ago) |
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Quote:
bodhisatta said: I always used foil and no micropore. I think micropore tape is useless. foil on sterilize foil off and save the foil it gets reused at least 10 times before it's too shitty.
I do all my PF style grows with gas exchange lids and no dry verm barrier. I just inoculate with agar by taking the lids off.

I actually read a thread where someone put some micropore underneath a scope and proved it did nothing to really stop contams. It's too big and some bacteria gets trapped on it's fibers but others slip right through, especially after a PC round.
Good luck Crypt, I'll be watching,
- Gyo
EDIT:
LCs are easy but you should do a PF tek and without contams before you try it. Save the last .5 cc of all your syringes and make a Liquid Culture and it'll multiply. I've gotten 8-10 CCs back from a .5 cc syringe using a Liquid Culture.
The problem with LCs are if there's a contam in the water you won't know until you shoot it on agar or knock up a jar and since you're not into agar yet I'd hold off on it.
Relative difficulty IMO (From easiest to hardest)
PF Tek Bulk Agar/LC/LI/Everything else.
Once you get a good PF tek and a good bulk going you'll be ready to tackle the microscopic side of growing. I made my first LCs before my first PF tek and they all turned out contam free but I didn't even have an SAB or a glovebox, I just propped a box up against two shoeboxes and worked in there.
I got REAL lucky .
Just like Mustard said, it's all about sterilitiy.
I used Cyber's LC tek with great success.
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20969325 - 12/12/14 11:05 PM (10 years, 1 month ago) |
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Quote:
taGyo said:
Quote:
bodhisatta said: I always used foil and no micropore. I think micropore tape is useless. foil on sterilize foil off and save the foil it gets reused at least 10 times before it's too shitty.
I do all my PF style grows with gas exchange lids and no dry verm barrier. I just inoculate with agar by taking the lids off.

I actually read a thread where someone put some micropore underneath a scope and proved it did nothing to really stop contams. It's too big and some bacteria gets trapped on it's fibers but others slip right through, especially after a PC round.
Good luck Crypt, I'll be watching,
- Gyo
EDIT:
LCs are easy but you should do a PF tek and without contams before you try it. Save the last .5 cc of all your syringes and make a Liquid Culture and it'll multiply. I've gotten 8-10 CCs back from a .5 cc syringe using a Liquid Culture.
The problem with LCs are if there's a contam in the water you won't know until you shoot it on agar or knock up a jar and since you're not into agar yet I'd hold off on it.
Relative difficulty IMO (From easiest to hardest)
PF Tek Bulk Agar/LC/LI/Everything else.
Once you get a good PF tek and a good bulk going you'll be ready to tackle the microscopic side of growing. I made my first LCs before my first PF tek and they all turned out contam free but I didn't even have an SAB or a glovebox, I just propped a box up against two shoeboxes and worked in there.
I got REAL lucky .
Just like Mustard said, it's all about sterilitiy.
I used Cyber's LC tek with great success.
I don't know what I did to earn such attention! But thank you for all your efforts, tag. I am sorry if I ask a lot of {silly} things
It's funny you did a scale of difficulty, I was gonna ask that lol. See I can search threads all day, but a lot of it is out dated and not all the recent stuff is backed thoroughly.
Also, I might make a liquid culture with next brf because there will be 1.0 cc left over after 9 jar inbox. But I might do seven. Synergy, magic, whoa.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20969332 - 12/12/14 11:07 PM (10 years, 1 month ago) |
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Sounds good .
Seven is my lucky number.
I'll be watching,

- Gyo
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20972451 - 12/13/14 06:46 PM (10 years, 1 month ago) |
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Today I prepared 9 more jars. I've yet to check my Original BRFs. I will do so in approx 1 more hour.
New set will be 3 wide mouths short and 6 wide mouths tall and skinny
All are 1/2 pint wide mouth.
Drilled 2/32 holes in the cardinal direction pattern around the rims. Take a break after all cleanup and then will prepare z jars.
Alas, A new thread will be started for this next BRF attempt, and I will still be updating this one until the end, however it may come.
New thread link coming.
Edited by Cryptology (12/13/14 08:21 PM)
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20972543 - 12/13/14 07:12 PM (10 years, 1 month ago) |
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-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20972836 - 12/13/14 08:11 PM (10 years, 1 month ago) |
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Quote:
Cryptology said: Today I prepared 9 more jars. I've yet to check my Original BRFs. I will do so in approx 1 more hour.
New set will be 3 wide mouths short and 6 wide mouths tall and skill
All are 1/2 pint wide mouth.
Drilled 2/32 holes in the cardinal direction pattern around the rims. Take a break after all cleanup and then will prepare z jars.
Alas, A new thread will be started for this next BRF attempt, and I will still be updating this one until the end, however it may come.
New thread link coming.
New jars are cooking.
Old jars are doing good, actually. Growth seems to have resumed at a normal pace with a lack of stink socks smell. I think I have removed the contaminated jars, but perhaps there will be more.
At this point the majority of my original jars are at about 60% colonization, a couple are less than 50%, but I believe they are now on a normal growth cycle with the temperature dialed in. Since they are now growing normally it appears as though the jars will colonize in 2 weeks or less.
Edited by Cryptology (12/13/14 08:21 PM)
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20972865 - 12/13/14 08:19 PM (10 years, 1 month ago) |
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Link to new BRF project - 2nd Project
This current project will still live on. Check back on both!
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20973087 - 12/13/14 09:12 PM (10 years, 1 month ago) |
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-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20981388 - 12/15/14 06:59 PM (10 years, 1 month ago) |
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Growth seems to have slowed down. I am not sure what I am doing wrong here, but at least there are no more ocntams? How much longer should I wait before doing something?
And when it's time to do something what should I do?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20982010 - 12/15/14 08:58 PM (10 years, 1 month ago) |
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Chances are if you didn't PC one correctly you didn't PC them all correctly. That's just the way the dice roll , Wait it out until you see contams.
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20983533 - 12/16/14 08:14 AM (10 years, 1 month ago) |
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Quote:
taGyo said: Chances are if you didn't PC one correctly you didn't PC them all correctly. That's just the way the dice roll , Wait it out until you see contams.
I hope that I don't prove myself wrong by calling you wrong, but.....
CHECK THESE OUT

Nearly all of them have a single pint, yet none of them are 100% colonized. Should I birth them, shred them, and spawn to bulk?
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20983564 - 12/16/14 08:29 AM (10 years, 1 month ago) |
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Those are all contaminated. Sorry.
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: wowimflabbergasted]
#20983571 - 12/16/14 08:31 AM (10 years, 1 month ago) |
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Quote:
wowimflabbergasted said: Those are all contaminated. Sorry.
Just out of curiosity, what tells you that? I wanna know!
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Kayone
UniversalTraveler


Registered: 12/05/14
Posts: 123
Loc: Lost in the, USA
Last seen: 8 years, 6 months
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20983591 - 12/16/14 08:39 AM (10 years, 1 month ago) |
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Fruiting will occur once myeclium is fully colonized. Thus, since your jar isnt visually fully colonized and you have fruits, the colonization has completed due to overtakings of contam.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20983596 - 12/16/14 08:40 AM (10 years, 1 month ago) |
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Pinning in BRF jars that soon before full colonization is a big sign. This is a healthy jar...
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: wowimflabbergasted]
#20983602 - 12/16/14 08:41 AM (10 years, 1 month ago) |
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Hwoever it's not that soon. They've been trying to colonize for over 5 weeks now. Which is why I am wondering thats a sign since its not soon.
Could it be the colonization is stalled and they've fruited where they can? I am only asking for my own personal knowledge.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20983641 - 12/16/14 08:54 AM (10 years, 1 month ago) |
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Wow yeah it stalled due to contams and the mushrooms are trying their hardest to grow as a last ditch effort to survive.
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: wowimflabbergasted]
#20983652 - 12/16/14 08:56 AM (10 years, 1 month ago) |
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Quote:
wowimflabbergasted said: Wow yeah it stalled due to contams and the mushrooms are trying their hardest to grow as a last ditch effort to survive.
Well, I will take your word for it and PC the jars and recycle. Once I pc for an hour or so.. is it safe to dump them in a garbage bag from inside my house? I only ask because I have a lot of pesky neighbors and I don't want to lose the jars.
Also, despite it being contaminated nothing smells bad
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