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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20952083 - 12/09/14 11:18 AM (10 years, 1 month ago) |
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Can you rephrase your question please?
Glad to hear it helped :]
-------------------- Gyo's Better Grows
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Acquario
Experience is Wisdom


Registered: 11/09/14
Posts: 811
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20952124 - 12/09/14 11:27 AM (10 years, 1 month ago) |
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Your stacks of jars look great! Here to follow and provide positive vibrations.
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Amateur Mycologists United [AMU Q&A Thread] - Ask any question here!
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20952901 - 12/09/14 02:17 PM (10 years, 1 month ago) |
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How and when do you determine a jar has stalled?
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Cryptology
Grand Inquirer

Registered: 10/30/14
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Acquario]
#20952906 - 12/09/14 02:18 PM (10 years, 1 month ago) |
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/
Edited by Cryptology (12/09/14 02:19 PM)
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Cryptology
Grand Inquirer

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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Acquario]
#20952910 - 12/09/14 02:19 PM (10 years, 1 month ago) |
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Quote:
Acquario said: Your stacks of jars look great! Here to follow and provide positive vibrations.
Thank you for stopping by my thread and offering encouragement! I will post more updates soon.
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taGyo
Strainiac/AMU



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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20952917 - 12/09/14 02:21 PM (10 years, 1 month ago) |
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Get a sharpie and draw a line around where the myc is. Come back in two days and you should see growth.
-------------------- Gyo's Better Grows
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Dominus fortunae meae sum
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Cryptology
Grand Inquirer

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Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20952936 - 12/09/14 02:25 PM (10 years, 1 month ago) |
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Quote:
taGyo said: Get a sharpie and draw a line around where the myc is. Come back in two days and you should see growth.
10-4 Desert Ranger
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Acquario
Experience is Wisdom


Registered: 11/09/14
Posts: 811
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20953111 - 12/09/14 02:59 PM (10 years, 1 month ago) |
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Quote:
Cryptology said: How and when do you determine a jar has stalled?
It can happen to anyone. Sometimes extra, extra patience is necessary
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Cryptology
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Acquario]
#20962163 - 12/11/14 01:54 PM (10 years, 1 month ago) |
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Updated: 12.11.2014
Signs of greenish / yellowish contam spots on bottom of two jars, and brownish like artifacts sticking to sides of these jars in small specks. These jars contained a lot of bottom moisture, enough to puddle. I am preparing to incinerate and trash so that when garbage man comes he doesn't get attacked by garbage full of possibly dangerous spores.
7 jars out 9 still in the game, or so it seems!
Also, just ordered a bunch of supplies and am going to be setting up Rye berry quart jars for mini monotub. Jars will have SFD under lids, and will be using PC as well as 27 qt mini mono for spawning to bulk in either coir or something else. Haven't decided. Any recommends for this secondary project will be nice.
Thread for second project - 2nd Project - Rye Berry & Cube: Part 1, Setup.
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taGyo
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20962336 - 12/11/14 02:43 PM (10 years, 1 month ago) |
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Think about what you could have improved in your sterile and brf formula, Progress, not perfection
-------------------- Gyo's Better Grows
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Cryptology
Grand Inquirer

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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20962375 - 12/11/14 02:51 PM (10 years, 1 month ago) |
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The only thing I didn't do is innoc in glove box because of how difficult it is to see how much is dispersed.
though, I have to admit maybe I've been checking them too frequently in the last couple of weeks because of their stalling, or what seemed to be stalling.
Thirdly, 77 degrees it is.
Also, I will try inoculating through a alcohol filled square sponge cut to nice quarter sized square that will sit over the innoc holes so that the needle passes through the alchol sponge (thus decomtaminating on its way in then the SFD. OF course I will flame sterilize in between jars still.
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taGyo
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20962391 - 12/11/14 02:55 PM (10 years, 1 month ago) |
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Flame sterilizes and alcohol sanitizes.
I iso'd my hands one day and touched an agar plate directly with one finger:

That showed up a little while after.
I inoculate some of my jars in open air, your problem was either lack of proper sterilization (Not 15 PSI or sterilized long enough) or a dirty needle or you didn't flame sterilize your needle. If you can think back, did you do anything differently with two jars?
-------------------- Gyo's Better Grows
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20962553 - 12/11/14 03:37 PM (10 years, 1 month ago) |
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Quote:
I am preparing to incinerate and trash so that when garbage man comes he doesn't get attacked by garbage full of possibly dangerous spores.
Poor guy, completely unprepared for the possibility someone's garbage might contain mold or bacteria 
Quote:
Also, I will try inoculating through a alcohol filled square sponge cut to nice quarter sized square that will sit over the innoc holes so that the needle passes through the alchol sponge (thus decomtaminating on its way in then the SFD. OF course I will flame sterilize in between jars still.
Flame sterilizing kills everything. Alcohol is ineffective against many contaminants. You could actually recontaminate your needle by sticking it through the alcohol soaked sponge. The chances of contaminating the needle between when you flame sterilize it and actually inject it is remote as long as it doesn't touch anything.
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taGyo
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Kizzle]
#20962562 - 12/11/14 03:38 PM (10 years, 1 month ago) |
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Oh shit, Kizzle's here.
-------------------- Gyo's Better Grows
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Cryptology
Grand Inquirer

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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20965684 - 12/12/14 08:27 AM (10 years, 1 month ago) |
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Quote:
taGyo said: Oh shit, Kizzle's here.
I tried summoning a greater deity and it worked! Welcome Kizz!
And Tagyo.. I am thinking back and I do remember this... The innoc holes were a little too small and when I stuck the larger needle through I really had to apply some pressure which would cause the needle to touch around the jar lid when I'd miss.
Also, now that I think of it.. when moving them around in their storage container I bumped the container a couple a times against a corner by accident and shook the jars pretty soundly a couple times. Though, it was not repeated and intentional shaking =(
I believe other jars are showing similar signs now too because I smell socks when I open their housing. I think I did bad, very bad.. and I had the temp too low for the first month so I didn't see any contams because the conditions weren't ideal for them to show, yet.
So...
If I do BRF again I will do my best provide them with earthquake proof stability. I will also enlarge innoc points slightly, and I will try to do them in the glove box....
However, I am going to be starting the Rye grain jars in the next coming days. So, I will have to do BRF another time. However, I will try to keep these jars alive, but I don't know if any of them will make it.
Edited by Cryptology (12/12/14 09:38 AM)
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taGyo
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20965930 - 12/12/14 09:37 AM (10 years, 1 month ago) |
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Trust me, Put the rye on hold. Bulk is harder and hurts even more when one thing goes wrong. And trust me, it will. I was literally right where you were a month ago when I started my grow log and I learned A LOT because I made a lot of mistakes and I feel like it's my duty to help those that made the same or similar mistakes.
You'll most likely make the rye jars and a few will contam because Rye is tricky your first two or three times. If you do go bulk please buy some Wild Bird Seed and follow this much easier tek:
6T WBS Tek
This will get you acclimated to grains in a very easy way. After you understand what you're doing and why you're doing it I would move on to Rye. It's RogerRabbit's favorite but comes with a lot of problems if you don't know how to prep it correctly.
When you do make those rye grain jars I suggest this configuration:

I use the smaller hole for inoculation and then stick a piece of Transpore or Micropore over it and leave the bigger ones out for gas exchange. 100 percent success rate. Your needle won't touch your grains so I like to shoot it all on one side to increase the chance of germination. After you inject DO NOT SHAKE. This will slow down germination by a few days because the spores need to find each other.
ON TO THESE JARS:
Just toss 'em if you see contam. Don't bother to let it grow and start again.
Can you show me your holes?
-------------------- Gyo's Better Grows
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Cryptology
Grand Inquirer

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Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20965967 - 12/12/14 09:47 AM (10 years, 1 month ago) |
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Quote:
taGyo said: Trust me, Put the rye on hold. Bulk is harder and hurts even more when one thing goes wrong. And trust me, it will. I was literally right where you were a month ago when I started my grow log and I learned A LOT because I made a lot of mistakes and I feel like it's my duty to help those that made the same or similar mistakes.
You'll most likely make the rye jars and a few will contam because Rye is tricky your first two or three times. If you do go bulk please buy some Wild Bird Seed and follow this much easier tek:
6T WBS Tek
This will get you acclimated to grains in a very easy way. After you understand what you're doing and why you're doing it I would move on to Rye. It's RogerRabbit's favorite but comes with a lot of problems if you don't know how to prep it correctly.
When you do make those rye grain jars I suggest this configuration:

As for the holes... I can't take a picture, but I can tell you that the holes were exactly, if not slightly smaller, than the circumference of the needle my syr's came with.
I use the smaller hole for inoculation and then stick a piece of Transpore or Micropore over it and leave the bigger ones out for gas exchange. 100 percent success rate. Your needle won't touch your grains so I like to shoot it all on one side to increase the chance of germination. After you inject DO NOT SHAKE. This will slow down germination by a few days because the spores need to find each other.
ON TO THESE JARS:
Just toss 'em if you see contam. Don't bother to let it grow and start again.
Can you show me your holes?
Well, interestingly enough I bought enough supply to do another BRF as well. I will do another batch of BRF and start my first batch of Rye either a day after or later in time depending on how I feel. I will take your advice and ahere with caution.
Edited by Cryptology (12/12/14 09:51 AM)
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taGyo
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: Cryptology]
#20965993 - 12/12/14 09:52 AM (10 years, 1 month ago) |
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,
Always up to you but these are just my personal experiences. Just found out my master KSSS jar had bacteria in it which is a bummer because it's contamed the rest of the culture I have growing.
Looks like I'll start that one from scratch
-------------------- Gyo's Better Grows
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
Loc: Zone 5
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20966002 - 12/12/14 09:53 AM (10 years, 1 month ago) |
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Quote:
taGyo said:
ON TO THESE JARS:
Just toss 'em if you see contam. Don't bother to let it grow and start again.
Can you show me your holes?
Can I use SFD in the BRF instead of Verm? If so would it be feasible to make the 4 innoc holes as per usual, perhaps larger, and then stick penny sized SFD siliconed to the underside of the lid instead of using verm? Or use that and verm?
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Cryptology
Grand Inquirer

Registered: 10/30/14
Posts: 165
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Re: Looking for tips and recommandations for Shroomery Setup. (moved) [Re: taGyo]
#20966014 - 12/12/14 09:55 AM (10 years, 1 month ago) |
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Quote:
taGyo said:
,
Always up to you but these are just my personal experiences. Just found out my master KSSS jar had bacteria in it which is a bummer because it's contamed the rest of the culture I have growing.
Looks like I'll start that one from scratch
That's another thing I must learn, but I not ready for, yet. - Master jars, Agar Teks, and cloning.
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