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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Best way to start a batch from 1 year old B+ spores [Re: psilocybeMAN]
    #20713085 - 10/16/14 08:44 PM (9 years, 5 months ago)

Quote:

psilocybeMAN said:
If you dont have access to agar, go ahead try an LC.




Not really an excuse. If you have access to the internet and/or a grocery store you can make all the agar you need. Its not really hard either. For the record I am not bashing your post or your assertion that contamed culture works outdoors, I just want to set the record straight. Agar is easier than pf tek :thumbup:

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OfflinepsilocybeMAN
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Re: Best way to start a batch from 1 year old B+ spores [Re: Pastywhyte]
    #20713101 - 10/16/14 08:46 PM (9 years, 5 months ago)

Yea, i edited that bit. You should always aim for clean cultures. I'm actually doing my first agar attempt with your tek. Going well :grin:.

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InvisiblePastywhyteMDiscord
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Re: Best way to start a batch from 1 year old B+ spores [Re: psilocybeMAN]
    #20713112 - 10/16/14 08:50 PM (9 years, 5 months ago)

Quote:

psilocybeMAN said:
Yea, i edited that bit. You should always aim for clean cultures. I'm actually doing my first agar attempt with your tek. Going well :grin:.




:rockon:

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Invisibleazur
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Re: Best way to start a batch from 1 year old B+ spores [Re: Pastywhyte]
    #20715422 - 10/17/14 12:25 PM (9 years, 5 months ago)

This whole thread is:doublefacepalm:


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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InvisibleMr. Alien
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Re: Best way to start a batch from 1 year old B+ spores [Re: psilocybeMAN]
    #20729856 - 10/20/14 04:31 PM (9 years, 5 months ago)

Quote:

psilocybeMAN said:
    If you dont have access to agar, go ahead try an LC. May or may not end up with a clean culture. Not the worst suggestion, just not the best.




Ok, i will try my dirty spores in a LC first, then inoculate 100 grain jars with it, thanks for the advice!!! I'm kidding :P.

Even if you inoculate a BRF to test if the LC is clean, by the time you see healthy colonization, bacteria or mold may start to divide and the next time you inoculate the LC into the 100 jars they all get contam. You are better and safer inoculating your dirty spores into non-aqueous substrates, at least in solid substrates like BRF cakes or grains, contams from spores do not thrive that easy.

Testing a LC it is still a good practice but it is more reliable if the LC that you are testing was inoculated with an agar wedge instead of spores.

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Invisibleazur
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Re: Best way to start a batch from 1 year old B+ spores [Re: Mr. Alien]
    #20729863 - 10/20/14 04:33 PM (9 years, 5 months ago)

I don't ever test lc. Only bit my ass once. But bit it BIG TIME!
Use agar for lc.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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Invisiblesaam234
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Re: Best way to start a batch from 1 year old B+ spores [Re: azur]
    #20729941 - 10/20/14 04:55 PM (9 years, 5 months ago)

I can't read azur's posts all I see is boobs

Btw thanks everyone you convinced me to use agar :thumbup:

Edited by saam234 (10/20/14 04:57 PM)

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