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Offlinepluteus
level-9 deviant

Registered: 08/12/03
Posts: 170
Loc: London area, UK
Last seen: 17 years, 6 months
Re: Mating spores [Re: micro]
    #2068463 - 11/03/03 05:38 PM (20 years, 6 months ago)

How about:

Brown, A.J. and L.A. Casselton (2001) Mating in mushrooms: increasing the chances but prolonging the affair, Trends in Genetics Vol17 No7 pp393-400

or a much more detailed account:

Casselton, L.A., and N.S. Olesnicky (1998) Molecular genetics of mating recognition in basidiomycete fungi, Microbiology and Molecular Biology Reviews Vol62 No1 pp.55-70

Of course the regulation of compatibility by mating type genes is only one level at which compatibility is controlled. If you want to explore within- and between-strain matings of monokaryons you should also read up on what is known about systems that regulate self-recognition, somatic incompatibility systems, and the functional context of genome co-operation / conflict.

I also find it helps to learn about the sometimes very different mating systems of other mushroom species to get a better idea of what P. cubensis in particular is doing.

By the way all you people who are experienced with mating single-spore cultures - WHAT IS YOUR METHOD OF SINGLE SPORE ISOLATION???

I am using a very laborious method (dilution, streaking, incubating, finding germinating spores under microscope, cutting out germinating spores on agar discs using spore cutter device, transferring, etc). What is everyone else doing?????

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OfflineSeraph
ShroomiN'SeraphiM
Female

Registered: 06/23/02
Posts: 183
Loc: South Flordia
Last seen: 6 years, 30 days
Re: Mating spores [Re: pluteus]
    #2069984 - 11/04/03 10:14 AM (20 years, 6 months ago)

thanks for the references.

Id also like to know the methods those here use?


--------------------
(\(\
( -.-)
o_(")(")

~nicole

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Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,298
Last seen: 7 months, 12 days
Re: Mating spores [Re: pluteus]
    #2070059 - 11/04/03 11:38 AM (20 years, 6 months ago)

Thanks for the hints - I am going to try Kazaa first  :wink: 


--------------------
-> Errors Are A Great Source Of Knowledge <-
-> It Is Not Important WHO Is Right But WHAT Is Right <-

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