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Amazon Shop for: ½ Pint Jars, Potato Dextrose

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Offlinecarnage
MycoVore
Registered: 02/25/03
Posts: 23
Loc: OHIO
Last seen: 7 years, 1 month
PDY Broth -- Reusable?
    #2067991 - 11/03/03 02:43 PM (13 years, 1 month ago)

If I were to make PDY (potato dextrose yeast) broth, grow the mycelium, and extract the active alkaloids out of the mycelium by pouring the contents of the jar over cheesecloth and letting the mycelium stay on top and the broth drain into a sterile pan, would I be able to put the broth that I just poured out back into the jar and reinoculate it, or do I have to prepare new media every time I strain out mycelium?


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Invisiblemicro
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Re: PDY Broth -- Reusable? [Re: carnage]
    #2068120 - 11/03/03 03:54 PM (13 years, 1 month ago)

You shouldn't reuse broth -- there will be waste products in it, and autoclaving it multiple times can caramalize the sugars.

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Invisibleshroomophile
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Re: PDY Broth -- Reusable? [Re: carnage]
    #2107719 - 11/14/03 10:57 PM (13 years, 28 days ago)

He's right ya know.


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Amazon Shop for: ½ Pint Jars, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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