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If I were to make PDY (potato dextrose yeast) broth, grow the mycelium, and extract the active alkaloids out of the mycelium by pouring the contents of the jar over cheesecloth and letting the mycelium stay on top and the broth drain into a sterile pan, would I be able to put the broth that I just poured out back into the jar and reinoculate it, or do I have to prepare new media every time I strain out mycelium?
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