Home | Community | Message Board


Vaposhop
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Potato Dextrose

Jump to first unread post. Pages: 1
Offlinecarnage
MycoVore
Registered: 02/25/03
Posts: 23
Loc: OHIO
Last seen: 7 years, 8 months
PDY Broth -- Reusable?
    #2067991 - 11/03/03 02:43 PM (13 years, 8 months ago)

If I were to make PDY (potato dextrose yeast) broth, grow the mycelium, and extract the active alkaloids out of the mycelium by pouring the contents of the jar over cheesecloth and letting the mycelium stay on top and the broth drain into a sterile pan, would I be able to put the broth that I just poured out back into the jar and reinoculate it, or do I have to prepare new media every time I strain out mycelium?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: PDY Broth -- Reusable? [Re: carnage]
    #2068120 - 11/03/03 03:54 PM (13 years, 8 months ago)

You shouldn't reuse broth -- there will be waste products in it, and autoclaving it multiple times can caramalize the sugars.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleshroomophile
ShroomitusFidelis
Male User Gallery

Registered: 08/20/02
Posts: 762
Loc: USA
Re: PDY Broth -- Reusable? [Re: carnage]
    #2107719 - 11/14/03 10:57 PM (13 years, 8 months ago)

He's right ya know.


--------------------
Once the mighty oak,was a nut who held his ground.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* PDY broth - what is "tender"? kidentropy 1,358 12 11/16/05 12:57 PM
by blackout
* P. Azurescens Specialty Agar/Broth buddig 1,410 6 06/01/02 09:13 AM
by buddig
* *NEW* Alien Broth Tek --- with pictures! metaophion 2,370 3 06/23/03 10:26 PM
by Static
* Vacuum-sealing your PDY micro 642 2 01/05/04 08:52 AM
by micro
* Trypticase Soy Broth - uses? Holydiver 892 3 05/20/08 06:08 PM
by MycoAu
* Letheen Broth HighHat 581 1 06/26/08 11:21 PM
by MycoAu
* nutrient broth question jvu81 1,670 1 09/09/04 05:32 PM
by ATWAR
* Glass/reusable petris: Anyone use them? altarego 709 3 11/04/01 07:36 PM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
553 topic views. 0 members, 5 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Crestline Sales - MycoPath
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.004 seconds on 16 queries.