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OfflineFrisky2x
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Registered: 09/26/14
Posts: 14
Last seen: 6 years, 11 months
how long can mycelium last underwater?
    #20674845 - 10/08/14 10:59 AM (9 years, 5 months ago)

I use mycelium taken from cakes and shake in sterile water then use a syringe to innculate many jars. My question is how long will mycelium last underwater in the fridge

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Invisiblefilthyknees
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Posts: 6,283
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Re: how long can mycelium last underwater? [Re: Frisky2x]
    #20674862 - 10/08/14 11:03 AM (9 years, 5 months ago)

this what you're looking for?

http://www.shroomery.org/forums/showflat.php/Number/16279876#16279876

search function in 10 seconds fyi


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

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Offlinepawnzy
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Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 14 days
Re: how long can mycelium last underwater? [Re: Frisky2x]
    #20674864 - 10/08/14 11:04 AM (9 years, 5 months ago)

can you tell us more information about your procedure.
Id say longer than 24 hours your suffocating it

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OfflineFrisky2x
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Registered: 09/26/14
Posts: 14
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Re: how long can mycelium last underwater? [Re: pawnzy]
    #20674918 - 10/08/14 11:19 AM (9 years, 5 months ago)

Thanks for the quick response. I have search for the answer I couldn't find info directly related to brf cakes mycelia.

my process is simple very effective
1sterlize water in glass jar
2let cool
3 I take dunked cakes and make my casing
4i save a small amount of strong mycelia and place inside Sterile water
5shake vigorously
6sterile needle goes in sterile water and suck up mycelia
7 I use about 4cc per jar I innculate. I must add I have 10-15 jars brf sterile ready to be inoculated
colonized in about 14days from inoculation

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Invisiblefilthyknees
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Re: how long can mycelium last underwater? [Re: Frisky2x]
    #20675007 - 10/08/14 11:42 AM (9 years, 5 months ago)

what you're describing is a grain liquid culture as mentioned in the link I posted :super:

make sure you make the transfer to the sterile water jar in a sab (you may know but thought worth mentioning since your post count...)


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

Edited by filthyknees (10/08/14 11:43 AM)

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OfflineGoldberg
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Re: how long can mycelium last underwater? [Re: filthyknees]
    #20675068 - 10/08/14 11:57 AM (9 years, 5 months ago)

Just g2g, why wouldn't you just leave out all those bs steps and g2g??

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OfflineRisco
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Re: how long can mycelium last underwater? [Re: Goldberg]
    #20675256 - 10/08/14 12:47 PM (9 years, 5 months ago)

Quote:

Goldberg said:
Just g2g, why wouldn't you just leave out all those bs steps and g2g??




Because he's inoculating BRF cakes

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OfflineGoldberg
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Re: how long can mycelium last underwater? [Re: Risco]
    #20675264 - 10/08/14 12:49 PM (9 years, 5 months ago)

Brf cakes!?!? Why!!!!
It's cool tho OP do what u want but if u want to save yourself a bunch of time and frustration, start growing the right way and switch to grains they will give you so much more versatility and in the end so much more yield.  Food for thought. And grains are fucking easy btw

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OfflineRisco
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Re: how long can mycelium last underwater? [Re: Goldberg]
    #20675288 - 10/08/14 12:58 PM (9 years, 5 months ago)

Maybe he doesn't have a PC. I'm not disagreeing with you at all. Just sayin

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OfflineFrisky2x
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Registered: 09/26/14
Posts: 14
Last seen: 6 years, 11 months
Re: how long can mycelium last underwater? [Re: Risco]
    #20675504 - 10/08/14 01:43 PM (9 years, 5 months ago)

Alright the real deal on brf cakes is this. Its the only way I have done it. I really want gp branch off,into wbs or g2g. I just haven't ahopped the supplies and I don't feel as comfy and warm as I do with my familier little  brf cakes! I've begun experimenting with new substrates and maybe even devolping a super cheap jar alternative. So I need a good mentor tho on the grains thing

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OfflineRisco
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Re: how long can mycelium last underwater? [Re: Frisky2x]
    #20675523 - 10/08/14 01:47 PM (9 years, 5 months ago)

There are some really good teks on here for grains if you  search for em. Or there are usually ballin links in the TC's sigs.

No one is forcing you to move on to grains, it's just the next logical step. If you have the materials for more BRF cakes and don't wanna spend more money, then more power to you. If you're just nervous about moving on but want to, then do it! Who cares if you fail a couple times and learn in the process. But if you have a PC and follow teks correctly, then you shouldn't have too much trouble making the next step.

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OfflinecronicrFacebook
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Re: how long can mycelium last underwater? [Re: Frisky2x]
    #20675593 - 10/08/14 02:07 PM (9 years, 5 months ago)

Quote:

Frisky2x said:
Alright the real deal on brf cakes is this. Its the only way I have done it. I really want gp branch off,into wbs or g2g. I just haven't ahopped the supplies and I don't feel as comfy and warm as I do with my familier little  brf cakes! I've begun experimenting with new substrates and maybe even devolping a super cheap jar alternative. So I need a good mentor tho on the grains thing



:standingby:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Invisiblewowimflabbergasted
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Re: how long can mycelium last underwater? [Re: Goldberg]
    #20675883 - 10/08/14 03:29 PM (9 years, 5 months ago)

Quote:

Goldberg said:
Brf cakes!?!? Why!!!!
It's cool tho OP do what u want but if u want to save yourself a bunch of time and frustration, start growing the right way and switch to grains they will give you so much more versatility and in the end so much more yield.  Food for thought. And grains are fucking easy btw



Because brf cakes are fun? They work? They're cheap? They teach noobs a lot about the mushroom life cycle? Not everyone is going for pounds at a time...sure grains are pretty simple but don't be all oooohhhh BRF CAKES WHAT THE FUCK ARE YOU THINKING?!?!


--------------------
TempMail | TempPMs
My Guide | MJ Grows


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OfflineGoldberg
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Re: how long can mycelium last underwater? [Re: wowimflabbergasted]
    #20675951 - 10/08/14 03:46 PM (9 years, 5 months ago)

I didn't say that but okay man, brf cakes are a pain in the royal ass even for actually especially for a noobs. Grains are fun-er

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OfflineRisco
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Re: how long can mycelium last underwater? [Re: Goldberg]
    #20675960 - 10/08/14 03:47 PM (9 years, 5 months ago)

Quote:

Goldberg said:
I didn't say that but okay man, brf cakes are a pain in the royal ass even for actually especially for a noobs. Grains are fun-er




Site your sources on this.

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OfflineGoldberg
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Re: how long can mycelium last underwater? [Re: Risco]
    #20675966 - 10/08/14 03:49 PM (9 years, 5 months ago)

Site my sources on what?!? Haha use the search function if u wanna find how brf cakes suck

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OfflinecronicrFacebook
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Re: how long can mycelium last underwater? [Re: Goldberg]
    #20675973 - 10/08/14 03:51 PM (9 years, 5 months ago)

FYI brf cakes have a far better BE then other setups, now let's keep this on topic


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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OfflineFrisky2x
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Registered: 09/26/14
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Last seen: 6 years, 11 months
Re: how long can mycelium last underwater? [Re: cronicr]
    #20678957 - 10/09/14 10:03 AM (9 years, 5 months ago)

I love how the topic has a life of its now! I created it and now I can't end it!!! Lol jk love you guys I'm no noob but I have been in retirement. Once I post pics it'll be cool

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InvisibleGhatti
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Re: how long can mycelium last underwater? [Re: Frisky2x]
    #20678968 - 10/09/14 10:07 AM (9 years, 5 months ago)

This is a loaded question. Are we talking about a cake or LC type setup?

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InvisibleMr. Alien
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Re: how long can mycelium last underwater? [Re: Frisky2x]
    #20678973 - 10/09/14 10:09 AM (9 years, 5 months ago)

Quote:

Frisky2x said:
Thanks for the quick response. I have search for the answer I couldn't find info directly related to brf cakes mycelia.

my process is simple very effective
1sterlize water in glass jar
2let cool
3 I take dunked cakes and make my casing
4i save a small amount of strong mycelia and place inside Sterile water
5shake vigorously
6sterile needle goes in sterile water and suck up mycelia
7 I use about 4cc per jar I innculate. I must add I have 10-15 jars brf sterile ready to be inoculated
colonized in about 14days from inoculation




Did you took myc from a cakes exposed to open air?

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