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cronicr



Registered: 08/07/11
Posts: 62,906
Loc: Van Isle
Last seen: 32 minutes, 11 seconds
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-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Mattarcher122
Stranger
Registered: 07/30/14
Posts: 13
Last seen: 10 years, 2 months
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Re: Pf tek done right [Re: Shea25]
#20357669 - 07/31/14 08:59 PM (10 years, 5 months ago) |
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Quote:
Shea25 said: Using this method I was constantly getting 6-9 gram first flushes. Around 13 grams dry per cake. the one in my avatar came out to 7.6 dry first flush
This is gonna sound real dumb but what is a flush
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greencrush420



Registered: 04/14/13
Posts: 1,014
Loc: U.S.A
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A crop. You can get two or three flushes off of of cakes by fruiting them, pick in all of the mushrooms, and then dunking them and fruiting again. Each time you pick all of the mushrooms, that is a "flush".
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hankhef
Stranger

Registered: 07/16/14
Posts: 2
Last seen: 10 years, 2 months
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Awesome and thanks to everyone on this site who has put their input in for us noobs to read and learn from. This is my first post and I'm here to tell ya that I just checked my very first PF TEK attempt after 3 days and found some beautiful white mycelium growing on my BRF / Vermiculite mix in 1 pint wide mouth jars!!! So stoked right now. I've ordered some half pint jars for the next attempt and I just hope that it continues to colonise ok in the full pints.... Made one mistake which I thought was fatal when I was making a spore syringe (psilicybe Cubensis) and that was not really thinking properly and for 4 out of the 5 syringes I used hot water straight off the stove. I thought it would have killed the spores but the jars used from this syringe are also growing mycelium well. I look forward to the next step and just wanna say it has been a lot of fun learning and experimenting... Thanks guys:)
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Sockadin



Registered: 01/03/10
Posts: 7,266
Last seen: 1 month, 22 days
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LOCK IT! STICKY IT!
Hankhef, that is awesome sauce. Feel free to make a new post. This thread should be archived and is 4 years old.
NO MORE
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cronicr


Registered: 08/07/11
Posts: 62,906
Loc: Van Isle
Last seen: 32 minutes, 11 seconds
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Re: Pf tek done right [Re: hankhef]
#20490400 - 08/28/14 07:17 PM (10 years, 4 months ago) |
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Welcome to shroomery
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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dancing monkey
Stranger



Registered: 04/19/14
Posts: 13
Loc: One Mountain away from Ha...
Last seen: 4 years, 3 months
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Re: Pf tek done right [Re: cronicr]
#20596056 - 09/21/14 07:48 AM (10 years, 3 months ago) |
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Ditto on great information, well written, and the photos where very helpful. appreciated the comments and all the positive tips.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,361
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Great thread. I just read the entire thing and have a few questions. If they were answered in the thread, forgive me, it's late and I am tired.
1. How do I ensure the levels in the grow chamber are 70-80 degrees at all times? Just heat the entire room?
2. What is the best gauge for humidity levels? I've read that most of the hygrometers suck for this method, so should I just not bother?
3. Shea was using a PMP, but RR's video and other guides use a Shotgun Chamber. What are the main issues I'll run into, if any, by using Shea's method with a Shotgun Chamber? The methods are VERY similar with this main difference, so this has me worried.
4. When using a 12/12 light/dark cycle, is a normal room with the blinds open/closed sufficient, or should I order a lighting system?
5. This might be related to #3, but how long after birthing am I spraying/fanning my cakes? RR's videos just say to do it a few times during the initial 24 hours.
6. Basic noob question: Can I buy the syringes ready to go? Or at least the LC to inject immediately? Or do I have to buy spores and make my own LC?
7. Are these the correct jars? http://www.aceREMOVEhardware.com/product/index.jsp?productId=3376864 (Delete the REMOVE tag in the root URL).
Thanks a lot to Shea and all the other wonderful posters in this thread!
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Munchauzen



Registered: 06/22/11
Posts: 14,348
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I'm short on time, so I'll just answer this one.
"6. Basic noob question: Can I buy the syringes ready to go? Or at least the LC to inject immediately? Or do I have to buy spores and make my own LC?"
You can buy premade spore syringes and inoculate with those, or use them to make an LC. You cannot buy premade LC syringes, because the LC contains live mycelium which contains active compounds that are illegal to possess (in most places).
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,361
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Thanks. I'm just learning the difference between LC and a regular MS injection (and how MS can create LC, etc.). I appreciate the help.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Quote:
stareatclouds said: (and how MS can create LC, etc.)
ehm, how MS should never create LC.
1. heat the room it's not that important, above 65 and below 90F and you'll be fine for fruiting. 2. hygrometers are the only way to measure it but unless you want to shell out some money for a nice calibratable synthetic hair hygrometer then don't even bother it's just another number that's going to cause you to panic needlessly. 3. use a SGFC the PMP is largely considered bogus. you can get results but for the price and hassle go with a SGFC if you want to try the PMP and be the next person telling people not to use one then go for it. 4. intense 6500k light the standard is a 26w(replaces 100w), incandescent replacement the spiral florescent. you want to find one in the 6500kalvin spectrum and you want it about a foot away from your grow. 5. no they don't you spray and fan as needed every day. mist then fan. only fan after you mist. fanning is not fae 6. don't make a LC start with spores. starting a LC with spores is asking for trouble, spore syringes cannot be made 100% sterile under most circumstances so putting spores into a nutrient rich liquid broth only serves to propagate contamination into every one of your jars, and you cannot visually tell if a LC is good to use so you're fucked. the only good way to make a LC is with a colonized agar wedge. 7. it says half pint wide mouth does it not
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,361
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hi bodhisatta,
Thanks for the response. I guess I'm still unsure about making LC, but that's fine since I'm a ways away from needing to do that.
RE #5: RR's videos on misting say "you want to do this several times over the next 2 hours." The caption says, "Repeat a few times over the next 24 hours" and then they move on. It's not very clear that I'm supposed to do this throughout the entire growth period.
RE #7: Yes, but I can't tell if they're tapered or not which is apparently very important. I was hoping someone has used that brand and would confirm.
Everything else I've read says spray every few hours and fan even more often. I'll follow those guides.
Thanks again!
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Munchauzen



Registered: 06/22/11
Posts: 14,348
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Quote:
6. don't make a LC start with spores. starting a LC with spores is asking for trouble, spore syringes cannot be made 100% sterile under most circumstances so putting spores into a nutrient rich liquid broth only serves to propagate contamination into every one of your jars, and you cannot visually tell if a LC is good to use so you're fucked. the only good way to make a LC is with a colonized agar wedge.
Would love more input from other users on this topic. I have never heard of this, but I managed to make several viable LCs from MS and get decent results.
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cronicr



Registered: 08/07/11
Posts: 62,906
Loc: Van Isle
Last seen: 32 minutes, 11 seconds
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Quote:
bodhisatta said:
Quote:
stareatclouds said: (and how MS can create LC, etc.)
ehm, how MS should never create LC.
1. heat the room it's not that important, above 65 and below 90F and you'll be fine for fruiting. 2. hygrometers are the only way to measure it but unless you want to shell out some money for a nice calibratable synthetic hair hygrometer then don't even bother it's just another number that's going to cause you to panic needlessly. 3. use a SGFC the PMP is largely considered bogus. you can get results but for the price and hassle go with a SGFC if you want to try the PMP and be the next person telling people not to use one then go for it. 4. intense 6500k light the standard is a 26w(replaces 100w), incandescent replacement the spiral florescent. you want to find one in the 6500kalvin spectrum and you want it about a foot away from your grow. 5. no they don't you spray and fan as needed every day. mist then fan. only fan after you mist. fanning is not fae 6. don't make a LC start with spores. starting a LC with spores is asking for trouble, spore syringes cannot be made 100% sterile under most circumstances so putting spores into a nutrient rich liquid broth only serves to propagate contamination into every one of your jars, and you cannot visually tell if a LC is good to use so you're fucked. the only good way to make a LC is with a colonized agar wedge. 7. it says half pint wide mouth does it not 
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,361
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Re: Pf tek done right [Re: cronicr]
#20644561 - 10/01/14 02:34 PM (10 years, 3 months ago) |
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I'm going to receive my syringes relatively soon and am planning on storing them in a paper bag in my fridge for future use. I was originally planning on injecting them directly into the substrate, but since this apparently has a low success rate, I'll be looking into LC.
I don't get how spores aren't supposed to kickstart or be a major component in creating LC? What am I inoculating the mixture with to begin the LC if not spores from the syringe?
Thanks.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Quote:
stareatclouds said: I'm going to receive my syringes relatively soon and am planning on storing them in a paper bag in my fridge for future use. I was originally planning on injecting them directly into the substrate, but since this apparently has a low success rate, I'll be looking into LC.
I don't get how spores aren't supposed to kickstart or be a major component in creating LC? What am I inoculating the mixture with to begin the LC if not spores from the syringe?
Thanks.
LC with spores has the lowest success rate. you inoculate a LC with a agar wedge. you put a small drop of spores onto a petri dish it will start to grow you will isolate mycelium from any contamination, put that on a new dish, then take a piece of clean mycelium on agar and start the LC with that. starting a LC with spores is a huge noob mistake. LC is just another step if you do spores to jars your jars will already be growing by the time the LC colonizes. Idk why anyone thinks going slower is a better idea. one more step, one more chance for contamination and one more week to grow.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,361
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Thank you for the reply. I'm still trying to understand this, so let me know where I'm wrong.
The spore syringes I ordered are just a straight LC with spores, correct? This has a low success rate. So instead, I would use those spore syringes in a Petri dish by themselves? Wouldn't they need agar to grow into mycelium?
Once I have the mycelium growing in my agar, would I just cut out a chunk and drop it into the jars, or do I mix that into LC and syringe inject?
Edited by stareatclouds (10/02/14 10:29 PM)
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Munchauzen



Registered: 06/22/11
Posts: 14,348
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selling LCs is illegal in most places, as they contain live mycelium. the spore syringes do not, though. its just spores suspended in water.
you can do the spore syringes on the agar, or straight into a brf cake. multispore inoculation on cakes is very noob friendly. where as agar is a little more advanced.
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E.FTS



Registered: 08/28/14
Posts: 467
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Re: Pf tek done right [Re: cronicr]
#20651730 - 10/03/14 12:49 AM (10 years, 3 months ago) |
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Quote:
cronicr said:

LOL. I am from Cleveland originally. I remember when that went down. Funny stuff.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,361
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Quote:
Munchauzen said: selling LCs is illegal in most places, as they contain live mycelium. the spore syringes do not, though. its just spores suspended in water.
you can do the spore syringes on the agar, or straight into a brf cake. multispore inoculation on cakes is very noob friendly. where as agar is a little more advanced.
Thank you. I'm planning on inoculating with my spore syringes and saving some to make LC at a later date. Can I inoculate, sterilize the needle with fire, and put them back in the fridge to store for later use?
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