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Offlinebengal
Bengal
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Registered: 12/17/13
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Agar / Master Culture Timeline Newbie Question
    #20630890 - 09/28/14 01:59 PM (10 years, 3 months ago)

So after about 10 successful monotub grows from MS inoculations, i'm finally about to dive into agar as I really would like to see some even genetics in my fruits/grows.  I've watched RR's videos on pouring agar and master culture slants, but I still have a few questions regarding the general overall process and timeline. I've been able to find some of the answers i'm looking for by searching the forum, but thought it would be best to just ask them all at once here.  Apologies if these questions are extremely basic or meant to be obvious. My goal is to simply get a couple of decent clones from my current grow, fruit them, then store the best one in a master culture slant for all my future grows.  Heres my questions. Any insight is as always greatly appreciated!

1. After pouring the agar, and placing the tissue sample onto the agar, how long will these last before going "bad"?  Should I store them at room temperature?

2. Regarding master culture slants, do I always store these in the fridge? Or wait for growth and then place in fridge? How long are these supposed to last once in the fridge?

3. How much agar would you make and how about how many petri dishes would you pour if you were going to just be taking samples from one or two fruits, given I intend on attempting isolates?  How many master culture slants?  I basically ask this to see how many dishes I can expect to lose from contams (I will be working in a SAB). 

4. Lastly, what does an average overall timeline look like for the whole process from taking tissue samples, to inoculating rye from the master culture slant?  Basically, can I pour all my agar now (petri dishes and master slants), keep all the dishes/master culture slants that aren't being used yet in the fridge, and use them once I need to?


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