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Offlineblojo02184
Big Red
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Registered: 05/15/13
Posts: 3,527
Loc: Maine
Last seen: 6 months, 21 days
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: kmetric]
    #20620999 - 09/26/14 10:38 AM (10 years, 3 months ago)

or none at all.

Usually when I am swiping plates, ill cool down my swiping instrument in my fresh plate, which in the process makes your tool slightly sticky from the agar it just liquefied.

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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge Flag
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: blojo02184]
    #20621271 - 09/26/14 12:10 PM (10 years, 3 months ago)

I dipped my loop in agar first, but only after it had cooled. Neat trick :smile:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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Offlineant707
not a puppet


Registered: 07/26/14
Posts: 579
Loc: northeast florida Flag
Last seen: 7 years, 11 months
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: NumeroEno]
    #20621737 - 09/26/14 02:34 PM (10 years, 3 months ago)

if you cool your freshly sterilized loop in your agar, it also coats the loop with agar, then when you touch your loop to your print.....spores stick to the agar. :thumbup:

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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: ant707]
    #20621974 - 09/26/14 03:38 PM (10 years, 3 months ago)

Yeah that's badass. Wish I had thought of that.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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OfflinePrimalSoup
hyperspatial illuminations
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Registered: 11/17/09
Posts: 13,568
Loc: PNW Flag
Last seen: 2 years, 4 months
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Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: NumeroEno]
    #20623540 - 09/26/14 09:53 PM (10 years, 3 months ago)

Often scraping the agar so that there are fissures exposes spore to more moisture (or at least a variety of moisture ratios) when you deposit spores into them.  I've gotten good germination sometimes from the divots vs the flat of the plate. :thumbup:

:peace:PS


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,972
Loc: Canada Flag
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Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: PrimalSoup]
    #20623560 - 09/26/14 09:57 PM (10 years, 3 months ago)

If you use a scalpel I like to just cut right into the agar, works great.

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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: Pastywhyte]
    #20623579 - 09/26/14 10:03 PM (10 years, 3 months ago)

I did kind of dig the loop into the agar a little bit.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
InvisibleNumeroEno
I come from the land of lizards
Male User Gallery


Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge Flag
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: NumeroEno]
    #20628069 - 09/27/14 09:35 PM (10 years, 3 months ago)

Holy shit. It's only been 48 hours and some of the spores have germinated. I definitely see a lot of bright white specks where I inoculated. Is this normal with scraping spores from a print on to agar? I wish I had this in a petri so I could take a nice macro of this, but I have no doubt that spores have already germinated.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
InvisibleSanguin3
Optimist

Registered: 10/19/13
Posts: 2,273
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. *DELETED* [Re: NumeroEno]
    #20628135 - 09/27/14 09:54 PM (10 years, 3 months ago)

Post deleted by Sanguin3

Reason for deletion: .

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Offlinemushpunx
Fungus Punk
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Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
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Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: Sanguin3]
    #20628163 - 09/27/14 10:05 PM (10 years, 3 months ago)

While were on the subject, my loop uses a thin nicotine wire, I cant really scrape up spores off my prints without a drop of sterile water on the print.

Do spores just stick to the loop lightly going in circular motion on a dry print? Just cant see em?


--------------------

Amateur Mycologists United
AMU Q&A

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OfflinePrimalSoup
hyperspatial illuminations
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Registered: 11/17/09
Posts: 13,568
Loc: PNW Flag
Last seen: 2 years, 4 months
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Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: mushpunx]
    #20628399 - 09/27/14 11:03 PM (10 years, 3 months ago)

I always flame the loop and cool it in the agar, this gets moisture on the loop which then picks up the spores - or at least that's the way it seems to me... :shrug:

:peace:PS


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

Extras: Filter Print Post Top
InvisibleNumeroEno
I come from the land of lizards
Male User Gallery


Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge Flag
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: mushpunx]
    #20629024 - 09/28/14 02:11 AM (10 years, 3 months ago)

Quote:

nicotine wire




Nichrome?  lol autocorrect is fuckin funny :rofl:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
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