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Salazar
Boomerang

Registered: 11/03/13
Posts: 27
Last seen: 8 years, 4 months
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Trich manifested in casing, need help isolating the cause
#20623410 - 09/26/14 09:18 PM (10 years, 3 months ago) |
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If you wanna skip to the nitty gritty just view the second paragraph, but im gonna give an in depth rundown of the entire process so someone may be able to pinpoint my error
wbs grain was soaked, cooked, and PCd at 15 psi for 1 hour. grain was inoculated with karo liquid culture, shook, and colonized in about 2 weeks. big coco brick was chopped into fourths, and put directly into 70qt FC that had been sprayed and wiped with lysol. tubs all have about 20 1/4 Inch wide holes stuffed snugly with polyfill for FAE. tubs also have opaque tape around the sides for darkness. boiling water was poured slowly over coco brick and verm in tubs individually. feild capacity was obtained and it was mixed. it was given a few hours to reach room temp then 6qts of healty white good smelling grain was added and mixed. a sterile trash bag was put overtop and suranwrap was wrapped all around the tub to keep fae from getting through the holes. tubs colonized at 73 F for 7 days. at 7 days all looked nice and white, smelled great, no infections of any sort. a casing of 50/50 peat and verm was applied in 1/ 4 inch to all the tubs but 1. the casing also had a handful of gypsum added, as well as 13 tsp of Hi Yeild hydrated lime to 20 pints of peat. this ratio was achieved by using red cabbage to test the ph.this basically involved taking a 1tsp sample of casing and adding it to 10 ml of red cabbage water which changes color according to pH. I found the pH to be about 7.5, tested 2 times. after that it was loaded into a boil bag and placed in a stockpot filled with water. it was heated for 1 hr. the middle of the bag held a temp of about 150 F but the edges of the bag were likely hotter(the water was boiling a bit at one point)
The black trash bag (which was folded during the wait to prevent contams from flying on it) was added back to the tub and placed over the casing. the casing sat for 4 days at 73 F in darkness with low FAE still. upon day 4, most tubs were showing mycelium poking through. one of the tubs allready had trich when the trash bag covers were removed that day. it was put in the room next to it to quarentine. the other tubs seemed fine so wax paper was added over the surface to initiate pinning and make a microclimate.the wax paper was not sterilized as I figured it would be sterile coming from the factory. but about 3 days after taking the trash bags off, two of the other tubs succumbed to trich and since then, another 3 are now fighting a losing battle. there are two healthy tubs left and they have hella pins now. if they survive its going to be a great flush. however I still need to identify exactly what caused my problems.
been making big mistakes out of desparation. been digging out infected areas trying to postpone the inevitable, likely spreading more spores everywhere, im fucking up. but I just need some input to see if anyone catches something I missed. i decided to check the ph of the casing again with a sample from the colonized sub and it read a ph of about 10.5. I have no idea how it ramped up like this but I can only assume my pH tests werent accurate. regardless I would think a high pH wouldnt cause mold to grow, but rather burn the mycelium, preventing pins and mold alike? im getting the opposite of that, lots of mold in the infected tubs, and lots of healthy pins in the un infected tubs? all with a super basic casing? im hella confused, their are a lot of factors and I hope I "pinned" them all;)
also im very aware that my digging into the subs has likely infected the house with trich, but I am hoping to remedy this by wiping down the whole room with moldex and setting up a hepa filter fan system, to pump fresh clean filtered air into the room and make it s positive pressure so no unclean air can sneak in through cracks. similarly a hepa filter is in the room allready constantly cycling clean air. all of this information is false and im using it in my fictional story I am writing. I am inquiring to get the best viewpoints to write an accurate novel.
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 24 days, 7 hours
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Re: Trich manifested in casing, need help isolating the cause [Re: Salazar]
#20623504 - 09/26/14 09:43 PM (10 years, 3 months ago) |
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1) trich is everywhere
2) your cabbage lied 
I've forsaken peat moss, cause I didn't wanna bother with pH; personally, I would recommend trying a coir/verm casing, I've had success with 50/50(plus gypsum), but I'm pretty sure the standard would be more like 30/70(coir/verm) 
edit:
also keep in mind, there's nothing sterile about spawning to bulk, especially your trash bags
Edited by MonkeyJesusFresco (09/26/14 09:44 PM)
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Salazar
Boomerang

Registered: 11/03/13
Posts: 27
Last seen: 8 years, 4 months
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Re: Trich manifested in casing, need help isolating the cause [Re: MonkeyJesusFresco]
#20623527 - 09/26/14 09:49 PM (10 years, 3 months ago) |
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yeah I thought about trying a coco case next go around, it seems so amazingly contam resistant. btw its worth noting that when I was digging around the infected spots their was hardly any white mushroom mycelium under the surface. it looked almost like uncolonized coco. it also crumpled really easy and didnt maintain its own shape. is this normal deep inside the sub?
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
Posts: 3,308
Loc: South East USA
Last seen: 24 days, 7 hours
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Re: Trich manifested in casing, need help isolating the cause [Re: Salazar]
#20623553 - 09/26/14 09:56 PM (10 years, 3 months ago) |
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oh....did you wait till the tub was 100% colonized before adding the casing?
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Salazar
Boomerang

Registered: 11/03/13
Posts: 27
Last seen: 8 years, 4 months
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Re: Trich manifested in casing, need help isolating the cause [Re: MonkeyJesusFresco]
#20623573 - 09/26/14 10:01 PM (10 years, 3 months ago) |
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it definitely seemed like it on the top and sides. the surfaces of the sub were nice and white with myc after just 7 days, however I had no way of telling that the insides werent the same way until I cut into it to get rid of the trich. my subs about 3.5 inches deep btw
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Sanguin3
Optimist

Registered: 10/19/13
Posts: 2,273
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Re: Trich manifested in casing, need help isolating the cause *DELETED* [Re: Salazar]
#20623643 - 09/26/14 10:22 PM (10 years, 3 months ago) |
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Post deleted by Sanguin3
Reason for deletion: .
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Salazar
Boomerang

Registered: 11/03/13
Posts: 27
Last seen: 8 years, 4 months
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Re: Trich manifested in casing, need help isolating the cause [Re: Sanguin3]
#20623682 - 09/26/14 10:34 PM (10 years, 3 months ago) |
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I was thinking it to just be the casing for a while too...but just today I noticed a bit of trich on the one that wasn't cased as well:/ perhaps this was just from the environment of being around all the infected ones, or maybe I'm making a mistake before I apply the casing? Thanks for the input by the way guys, it helps loads
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 3 hours, 5 minutes
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Re: Trich manifested in casing, need help isolating the cause [Re: Salazar]
#20623788 - 09/26/14 11:04 PM (10 years, 3 months ago) |
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i'd blame your spawn
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Salazar
Boomerang

Registered: 11/03/13
Posts: 27
Last seen: 8 years, 4 months
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Re: Trich manifested in casing, need help isolating the cause [Re: cronicr]
#20623822 - 09/26/14 11:15 PM (10 years, 3 months ago) |
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do you think bad grain could actually spawn to a tub and not show any signs of causing infection until day 11 of the colonization? (7 for substrate coln, 4 for casing coln). IF so that really extends my parameters of what I would have considered mold would do :/
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 3 hours, 5 minutes
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Re: Trich manifested in casing, need help isolating the cause [Re: Salazar]
#20623830 - 09/26/14 11:18 PM (10 years, 3 months ago) |
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yes it could easily just have been travelling along with your myc and your just not seeing it spore until it has colonized
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Salazar
Boomerang

Registered: 11/03/13
Posts: 27
Last seen: 8 years, 4 months
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Re: Trich manifested in casing, need help isolating the cause [Re: cronicr]
#20631934 - 09/28/14 05:43 PM (10 years, 3 months ago) |
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I would consider that possibility, but the same LC's used to knock up the grain is used for PF's as well and they have been doing just fine.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
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Re: Trich manifested in casing, need help isolating the cause [Re: Salazar]
#20632154 - 09/28/14 06:37 PM (10 years, 3 months ago) |
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Quote:
Salazar said: do you think bad grain could actually spawn to a tub and not show any signs of causing infection until day 11 of the colonization? (7 for substrate coln, 4 for casing coln). IF so that really extends my parameters of what I would have considered mold would do :/
Yes. It's not uncommon either. It's one of the reasons you should let your bulk substrate colonize in a transparent container if possible. The light helps trigger sporulation so you can catch it sooner.
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