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imaginaryMe
IThinkThereforeIAm



Registered: 11/27/10
Posts: 329
Loc: PNW
Last seen: 2 years, 22 days
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Plans for Ps. Cyanescens Mass Inoculation
#20623253 - 09/26/14 08:46 PM (10 years, 3 months ago) |
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So my friend and I have been discussing how to exponentially expand a cyan patch he has in his backyard. I'm making this thread to see what input you guys have, as well as document the projects to come. Thus it will eventually serve as a grow log of sorts.
I will start by saying some of the cultivation methods will be slightly improvised/experimental thus I will document everything to the best of my abilities.
The first step of the plan is to produce LC from his current patch. Even though agar is great, for simplicity we're opting to stay away from it. We will make the LC by performing a biopsy on the inner tissue of a few cyan stems. We plan to fill a syringe with LC, tear the stem of a fresh specimen open lengthwise, scrape some of the inner stem tissue with the tip of the needle, put the needle into the jar and free the tissue from inside the needle by injecting the LC in the syringe into the jar of LC. We are probably going to use Karo LC supplemented with some water from boiling alder chips. We will repeat this process to yield about 6 jars of LC.
Next, we have to test the LC out to make sure they are clean. I'm expecting a few to contaminate hence the redundancy of 6 jars. We will inject BRF cakes supplemented with sawdust with some of the LC.
After verifying the LC is clean, we will have a master culture. From there we plan to make a few dozen BRF cakes to use as spawn material, as well as make a bunch more LC. The spawn would be used to inoculate a few personal patches, and the LC will be used for mass inoculation.
I have seen a thread where Neosporen used cases of water bottles and put LC in the bottles. My question is, was the water just there to dilute the mycelium or did he add nutrients to the water bottles so the mycelium could colonize more?
If you guys think this would work, we were planning on adding a little bit of karo to each bottle, and adding a few cc's of clean LC to each bottle and letting it colonize. I know I will get contams in some, but these bottles will be used to spray outdoor wood chip beds in the spring and the contams will likely die off outdoors. The goal here is quantity, not quite quality. Do you guys think that would work?
What advice do you guys have? Any holes in my plan or anything you think would work better?
I know the proper way would be agar > grain >chips but I'm trying to do this easily and on the cheap, and I only have limited access to a PC that I gave away to someone from my days playing with cubensis.
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Lot's of Ps. Cyan Spore Swabs. Interested in Other PNW Wood Lovers or Edibles. PM Me!
Edited by imaginaryMe (09/26/14 08:54 PM)
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: imaginaryMe]
#20623333 - 09/26/14 09:01 PM (10 years, 3 months ago) |
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Stay away from BRF cakes.
Tiger dropping clones on agar is as simple as it fucking gets.
Use the tiger drop to inoculate wood chip bags.
Use full colonized wood chips to inoculate whatever the fuck you want.
6 glad mini rounds will get you 6 giant ass bags of wood chips.
Minirounds, polyfil, MEA powder, wood chips, spawn bags. PC. Done.

I just did all your planning for you.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber
The Shmuvbox. - The Old TC's Like it
Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.
"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT
Pastywhite's Easy Agar Tek (PastyPlates)
Tiger Drop Video Demos By munchauzen
Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson
EZEKIEL 23:20
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: elasticaltiger]
#20623354 - 09/26/14 09:06 PM (10 years, 3 months ago) |
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I'm just saying you remove all of your fucking 'redundancy' bullshit simply by using mini rounds. Not only that, but you don't even have to clone, you can take a clean spore print or swab and get even higher success rates.
Why worry about your LC being clean when you can drop the tissue onto a pasty plate and let it colonize out for like 2 days to make sure it's clean. You skip the entire testing phase AND you get to see how many different strains are actually working in the mycelium you're sampling.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber
The Shmuvbox. - The Old TC's Like it
Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.
"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT
Pastywhite's Easy Agar Tek (PastyPlates)
Tiger Drop Video Demos By munchauzen
Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson
EZEKIEL 23:20
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imaginaryMe
IThinkThereforeIAm



Registered: 11/27/10
Posts: 329
Loc: PNW
Last seen: 2 years, 22 days
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: elasticaltiger]
#20623430 - 09/26/14 09:24 PM (10 years, 3 months ago) |
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Well the issue is I will only have access to a PC for a day or two. Thus I'll use it to make the initial LC jars. Hence why I'd use BRF cakes instead of grain. Cloning is to avoid the contams which are bound to be present from outdoor prints. Your method seems great for cubes and I'll probably try it if I ever get back into cultivation, but it seems like the LC method with a few jars would be a bit easier than multiple transfers on agar to get rid of contams. The point of the mass amounts of LC is so we can go guerilla on the town and spray LC anywhere that looks suitable and potentially produce hundreds of patches rather than working with spawn for every single one of them.
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Lot's of Ps. Cyan Spore Swabs. Interested in Other PNW Wood Lovers or Edibles. PM Me!
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: imaginaryMe]
#20623471 - 09/26/14 09:34 PM (10 years, 3 months ago) |
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Ok. Uhm... No...
There are no multiple transfers. When I cloned myc to pasty plates I got 5 out of 7 clean and good for spawn making on the first try.
I don't know why n00bs are so afraid of agar all the time. You see more work because it's a flat dish instead of a giant mass of liquid.
I see less work because less time is spent worrying about your LC and testing. By the time all your 'testing' is done you could have done my plan once and be half way through your second round if not finished with two rounds.
There is nothing about what I told you just now that is impractical for any of the reasons you have just given me (even the PC for 1 day only thing)
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber
The Shmuvbox. - The Old TC's Like it
Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.
"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT
Pastywhite's Easy Agar Tek (PastyPlates)
Tiger Drop Video Demos By munchauzen
Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson
EZEKIEL 23:20
Edited by elasticaltiger (09/26/14 09:34 PM)
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imaginaryMe
IThinkThereforeIAm



Registered: 11/27/10
Posts: 329
Loc: PNW
Last seen: 2 years, 22 days
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: elasticaltiger]
#20623496 - 09/26/14 09:40 PM (10 years, 3 months ago) |
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I'm not scared of agar, I've worked with it quite a bit. I'm just wanting to keep it simple, and like I said, I want to mainly produce mass amounts of LC to inoculate everywhere.
The print would be from an outdoor grow bed so there probably would be contams, and I probably would have to do multiple transfers..
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Lot's of Ps. Cyan Spore Swabs. Interested in Other PNW Wood Lovers or Edibles. PM Me!
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: elasticaltiger]
#20623514 - 09/26/14 09:45 PM (10 years, 3 months ago) |
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So why wouldn't you just buy a 20 dollar PC for this? You can make a lot of agar plates and sterile pints of water for liquid inoculant whenever you feel like it with a cheap PC. If you've got a private woodlover patch you should invest a little in it.
That said, I hope whatever you end up doing works for you
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: imaginaryMe]
#20623516 - 09/26/14 09:46 PM (10 years, 3 months ago) |
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Quote:
imaginaryMe said: I'm not scared of agar, I've worked with it quite a bit. I'm just wanting to keep it simple, and like I said, I want to mainly produce mass amounts of LC to inoculate everywhere.
The print would be from an outdoor grow bed so there probably would be contams, and I probably would have to do multiple transfers..
I thought you said you were going to clone and not print?
If you do wood chips you are guaranteed a shit load of mushies. Why even chance LC?
Though I completely respect the right to go that route. Is there anyone else out there who can elaborate more on what he is asking about specifically rather than trying to cram agar down his throat like I always do?
Also, if a plate contams, just don't use it. Half decent technique will keep you from having to transfer.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber
The Shmuvbox. - The Old TC's Like it
Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.
"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT
Pastywhite's Easy Agar Tek (PastyPlates)
Tiger Drop Video Demos By munchauzen
Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson
EZEKIEL 23:20
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imaginaryMe
IThinkThereforeIAm



Registered: 11/27/10
Posts: 329
Loc: PNW
Last seen: 2 years, 22 days
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: elasticaltiger]
#20623542 - 09/26/14 09:54 PM (10 years, 3 months ago) |
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The initial plan was to clone, I thought you were saying go from a print to a plate, not to clone. Misunderstanding.
Neosporen had luck spraying LC in suitable landscaped areas. That way I can make cases and cases of water bottles of LC and just go everywhere spraying them wherever I think they will survive. This saves me the step of creating the actual beds, and I can just walk around town spraying whatever I want as opposed to mixing colonized chips in. Here's a video of a patch he made spraying LC
I know the benefits of agar, but eventually I'd have to turn that agar into LC and shoot test jars anyways, so why not just skip the agar?
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Lot's of Ps. Cyan Spore Swabs. Interested in Other PNW Wood Lovers or Edibles. PM Me!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: imaginaryMe]
#20623631 - 09/26/14 10:20 PM (10 years, 3 months ago) |
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imaginaryMe
IThinkThereforeIAm



Registered: 11/27/10
Posts: 329
Loc: PNW
Last seen: 2 years, 22 days
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: MudaFuka]
#20623683 - 09/26/14 10:35 PM (10 years, 3 months ago) |
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If only I had a dope blender like that
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Lot's of Ps. Cyan Spore Swabs. Interested in Other PNW Wood Lovers or Edibles. PM Me!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: imaginaryMe]
#20623707 - 09/26/14 10:41 PM (10 years, 3 months ago) |
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Normally I would say inoculating a liquid culture with clone tissue is a bad idea especially from a wild clone but since you are using it to inoculate wild outdoor beds I would just go for it without even testing your LC first. If it doesn't work its not like you are wasting any spawn or substrate materials.
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imaginaryMe
IThinkThereforeIAm



Registered: 11/27/10
Posts: 329
Loc: PNW
Last seen: 2 years, 22 days
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: MudaFuka]
#20625286 - 09/27/14 11:07 AM (10 years, 3 months ago) |
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Ok so if I were to use agar, would it be better to transfer to jars of wbs or jars of sawdust? I've never used bags before, so I want to use jars. Don't want to have buy a heat sealer or anything for the bags. Jars I can source cheap, and am plenty familiar with silicone injector ports and the like.
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Lot's of Ps. Cyan Spore Swabs. Interested in Other PNW Wood Lovers or Edibles. PM Me!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: imaginaryMe]
#20625367 - 09/27/14 11:25 AM (10 years, 3 months ago) |
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I thought you just wanted to make some liquid you could use out to inoculate out door patches. Why bother with jars at all.
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imaginaryMe
IThinkThereforeIAm



Registered: 11/27/10
Posts: 329
Loc: PNW
Last seen: 2 years, 22 days
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: MudaFuka]
#20625391 - 09/27/14 11:31 AM (10 years, 3 months ago) |
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Well we want both tons LC and some actual spawn so we can guarantee the success of a few personal patches.
My understanding for the spawn is agar > grain > wood chips. Do people sterilize or pasteurize the wood chips?
For the LC we'll go agar > LC > more LC
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Lot's of Ps. Cyan Spore Swabs. Interested in Other PNW Wood Lovers or Edibles. PM Me!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: imaginaryMe]
#20625405 - 09/27/14 11:35 AM (10 years, 3 months ago) |
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I would go agar to grain rather than sawdust. I wouldn't sterilize wood chips and I'm not really sure if they even need to be pasteurised for an out door grow.
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imaginaryMe
IThinkThereforeIAm



Registered: 11/27/10
Posts: 329
Loc: PNW
Last seen: 2 years, 22 days
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Re: Plans for Ps. Cyanescens Mass Inoculation [Re: MudaFuka]
#20625425 - 09/27/14 11:39 AM (10 years, 3 months ago) |
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Ok cool that's what I thought but just wanted to verify. Just gotta start sourcing my supplies now. Luckily I know where to get agar, everything else is easy to find. I'll keep you guys updated. Thanks for the help.
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Lot's of Ps. Cyan Spore Swabs. Interested in Other PNW Wood Lovers or Edibles. PM Me!
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