|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
blojo02184
Big Red



Registered: 05/15/13
Posts: 3,527
Loc: Maine
Last seen: 6 months, 21 days
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: kmetric]
#20620999 - 09/26/14 10:38 AM (10 years, 3 months ago) |
|
|
or none at all.
Usually when I am swiping plates, ill cool down my swiping instrument in my fresh plate, which in the process makes your tool slightly sticky from the agar it just liquefied.
|
NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: blojo02184]
#20621271 - 09/26/14 12:10 PM (10 years, 3 months ago) |
|
|
I dipped my loop in agar first, but only after it had cooled. Neat trick
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
|
ant707
not a puppet


Registered: 07/26/14
Posts: 579
Loc: northeast florida
Last seen: 7 years, 11 months
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: NumeroEno]
#20621737 - 09/26/14 02:34 PM (10 years, 3 months ago) |
|
|
if you cool your freshly sterilized loop in your agar, it also coats the loop with agar, then when you touch your loop to your print.....spores stick to the agar.
|
NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: ant707]
#20621974 - 09/26/14 03:38 PM (10 years, 3 months ago) |
|
|
Yeah that's badass. Wish I had thought of that.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
|
PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 2 years, 4 months
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: NumeroEno]
#20623540 - 09/26/14 09:53 PM (10 years, 3 months ago) |
|
|
Often scraping the agar so that there are fissures exposes spore to more moisture (or at least a variety of moisture ratios) when you deposit spores into them. I've gotten good germination sometimes from the divots vs the flat of the plate. 
PS
--------------------
if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: PrimalSoup]
#20623560 - 09/26/14 09:57 PM (10 years, 3 months ago) |
|
|
If you use a scalpel I like to just cut right into the agar, works great.
|
NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: Pastywhyte]
#20623579 - 09/26/14 10:03 PM (10 years, 3 months ago) |
|
|
I did kind of dig the loop into the agar a little bit.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
|
NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: NumeroEno]
#20628069 - 09/27/14 09:35 PM (10 years, 3 months ago) |
|
|
Holy shit. It's only been 48 hours and some of the spores have germinated. I definitely see a lot of bright white specks where I inoculated. Is this normal with scraping spores from a print on to agar? I wish I had this in a petri so I could take a nice macro of this, but I have no doubt that spores have already germinated.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
|
Sanguin3
Optimist

Registered: 10/19/13
Posts: 2,273
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. *DELETED* [Re: NumeroEno]
#20628135 - 09/27/14 09:54 PM (10 years, 3 months ago) |
|
|
Post deleted by Sanguin3
Reason for deletion: .
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: Sanguin3]
#20628163 - 09/27/14 10:05 PM (10 years, 3 months ago) |
|
|
While were on the subject, my loop uses a thin nicotine wire, I cant really scrape up spores off my prints without a drop of sterile water on the print.
Do spores just stick to the loop lightly going in circular motion on a dry print? Just cant see em?
--------------------

Amateur Mycologists United
AMU Q&A
|
PrimalSoup
hyperspatial illuminations



Registered: 11/17/09
Posts: 13,568
Loc: PNW
Last seen: 2 years, 4 months
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: mushpunx]
#20628399 - 09/27/14 11:03 PM (10 years, 3 months ago) |
|
|
I always flame the loop and cool it in the agar, this gets moisture on the loop which then picks up the spores - or at least that's the way it seems to me... 
PS
--------------------
if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
|
NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
|
Re: First time ever growing from a print. Wanna make sure I'm about to do it right. [Re: mushpunx]
#20629024 - 09/28/14 02:11 AM (10 years, 3 months ago) |
|
|
Quote:
nicotine wire
Nichrome? lol autocorrect is fuckin funny
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
|
|