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OfflineGiles Rokery
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Registered: 09/25/14
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Purple Ring Contam?
    #20617079 - 09/25/14 01:11 PM (10 years, 3 months ago)

I'm looking for some help on a consensus that this is a neurospora contam. I have several strains inoculated onto rye berries. In every case the Mexican sub-strain has colonized much quicker than the others only to result in a pale purple discoloration. The purple coloring is NOT uniform throughout the jar, it appears to manifest about a week after inoculation and then abate after several days, resulting in a purple ban around the jars.  Notice that this banding even occurs at a relatively similar height in all of the jars.
Another notable characteristic of this strain, colonization begins vigorously from the top down before stalling an inch or two above the bottom of the jars. This leads me to believe the stalling a is a result of CO2 or O2 exchange.
I've included a few photos and would appreciate any opinions or observations. Thx.





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OfflineKizzle
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Re: Purple Ring Contam? [Re: Giles Rokery]
    #20621279 - 09/26/14 12:11 PM (10 years, 3 months ago)

That's Fusarium. It's probably not your fault though. It looks like the syringe you used was contaminated with it.


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Offlinehamburgerhill
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Registered: 09/07/14
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Re: Purple Ring Contam? [Re: Kizzle]
    #20622768 - 09/26/14 06:47 PM (10 years, 3 months ago)

so with something like that is it all no good then?

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OfflineKizzle
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Re: Purple Ring Contam? [Re: hamburgerhill]
    #20623242 - 09/26/14 08:44 PM (10 years, 3 months ago)

No good. There's not even any actual mushroom mycelium in them.


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OfflineGiles Rokery
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Re: Purple Ring Contam? [Re: Kizzle]
    #20625614 - 09/27/14 12:29 PM (10 years, 3 months ago)

Thx. Is fusarium ubiquitous in the local environment?  All of the jars were noc'd from some LC I made via mycelium into karo water.  I pulled the initial mycelium off of a spore to rye growth.

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OfflineKizzle
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Re: Purple Ring Contam? [Re: Giles Rokery]
    #20627388 - 09/27/14 06:52 PM (10 years, 3 months ago)

It's often present on unsterilized grain so if you pour a bag of grain into a pan indoors or something the dust it produces is a major source of it. Soil and plants are other common sources.

Also some species don't change color on MEA or PDA so if you inoculated your LC with a culture that used one of those kinds of agar it can easily be mistaken for mushroom mycelium.


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