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OfflineCloner
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Question about innoculating jars that did not grow after innoculating once
    #20603205 - 09/22/14 08:02 PM (10 years, 3 months ago)

I have ten 1/2 pint jars that I did with the BRF PFTEK and with Golden Teacher spore syringe that I got from a sponser. It was my first attempt that I started over four weeks ago now and so far nothing has grown in any of them. No contamination and no myc.  At this point I don't know if the spores were bad or if I did something wrong. So at this point I am thinking that they probably won't grow. So I was wondering if it would be safe to inject with a new syringe or if I should just empty the jars and go through the sterilization step again.
The reason I ask is I just looked at my last syringe that I haven't used and it has numerous pieces of white stringy stuff in it. From searching the forums it seems like the spore probably started to germinate. I was planning on using that syringe in a few weeks as I just inoculated a New set of jars a little over a week ago with some Amazonian strand and so I am still waiting for those to spawn as well. So can I just inject those first set of jars? Or will the syringe still be viable if I hold onto it for a couple of weeks? Or should I empty those jars and try again?
Sorry if I am rambling on but any help is very much appreciated. Still hoping to get a first successful grow sometime soon.

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OfflineMonkeyJesusFresco
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Re: Question about innoculating jars that did not grow after innoculating once [Re: Cloner]
    #20603773 - 09/22/14 09:47 PM (10 years, 3 months ago)

empty those jars and try again :super:

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InvisibleSanguin3
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Re: Question about innoculating jars that did not grow after innoculating once *DELETED* [Re: MonkeyJesusFresco]
    #20603903 - 09/22/14 10:09 PM (10 years, 3 months ago)

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OfflinePsilicon
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Re: Question about innoculating jars that did not grow after innoculating once [Re: Sanguin3]
    #20604308 - 09/22/14 11:25 PM (10 years, 3 months ago)

:whathesaid::whathesaid:

Plus, at least half the time I have jars that didn't do anything, when I empty the jars out it becomes obvious it was because of a germinated endospore.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflineCloner
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Re: Question about inoculating jars that did not grow after inoculating once [Re: Psilicon]
    #20605232 - 09/23/14 08:58 AM (10 years, 3 months ago)

Ok thanks for the replies. Is it alright to reuse the BRF mixture in them or just toss out completely? Also I used organic BRF and organic verm using the 2-1-1 method. 2 cups of verm to 1 cup BRF and 1 cup water. Sterilized in a pressure cooker at 15 psi for an hour. All of the jars had the four holes in the tops that were then covered with masking tape during their time in the pc. I then waited around 24 hours and then I then used a sab to inoculate the 4 points with 1cc in each. This was the same with both of my two attempts with the only difference being the first attempt 4-5 weeks ago were in regular 1/2 pint jars and the second batch are in 1/2 pint wide mouth jars. I made sure to sterilize the syring in an alcohol lamp after each jar. So at this point I wonder if it is the syringes or if I possibly screwed something up. I am hoping my 2nd attempt will grow something as I have been trying to follow the tek as close as possible. Right now those jars have been sitting in room temperature Between 68 and 77 degrees since it has been getting cooler out. So am I missing something or is this just the hit and miss that people run into? Also what should I look for if it is an issue with endospore contamination? I know just looking at the jars right now they do not look like anything has been growing in them.

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InvisibleSanguin3
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Re: Question about inoculating jars that did not grow after inoculating once *DELETED* [Re: Cloner]
    #20605292 - 09/23/14 09:25 AM (10 years, 3 months ago)

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OfflineCloner
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Re: Question about inoculating jars that did not grow after inoculating once [Re: Sanguin3]
    #20605314 - 09/23/14 09:36 AM (10 years, 3 months ago)

It was 1cc in each hole with about 1ml in each jar. And I did put foil over each jar as well before putting them in my PC. Do I not need the tape over the holes? I used it so I could keep them sealed. When I inoculated them I just pulled the tape back, injected the hole and taped the hole again. I figured I would remove the tape completely once I started to see myc growth first if I can ever get to that point.

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InvisibleSanguin3
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Re: Question about inoculating jars that did not grow after inoculating once *DELETED* [Re: Cloner]
    #20605330 - 09/23/14 09:43 AM (10 years, 3 months ago)

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OfflineCloner
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Re: Question about inoculating jars that did not grow after inoculating once [Re: Sanguin3]
    #20605346 - 09/23/14 09:52 AM (10 years, 3 months ago)

Ah ok. I know it was just one of the lines under each ml is what I put in each hole. So a 10ml syringe I got 10 jars out of. Well I am still hoping my Amazon's will grow but I plan on dumping the first ten and going through it again so I can use my last syringe since it looks like myc has started to grow in the syringes already.
Thanks for the help. I'll make sure to post how it works out for me.

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InvisibleSanguin3
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Re: Question about inoculating jars that did not grow after inoculating once *DELETED* [Re: Cloner]
    #20605368 - 09/23/14 10:01 AM (10 years, 3 months ago)

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OfflineCloner
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Re: Question about inoculating jars that did not grow after inoculating once [Re: Sanguin3]
    #20605404 - 09/23/14 10:15 AM (10 years, 3 months ago)

Ah that's good to know. I guess I'll get better at it the more I try. I keep learning more the more I try and keep researching. Thanks again

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InvisibleSanguin3
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Re: Question about inoculating jars that did not grow after inoculating once *DELETED* [Re: Cloner]
    #20605594 - 09/23/14 11:05 AM (10 years, 3 months ago)

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OfflineCloner
The Cloner (eventually)
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Registered: 05/19/14
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Re: Question about inoculating jars that did not grow after inoculating once [Re: Sanguin3]
    #20607155 - 09/23/14 04:36 PM (10 years, 3 months ago)

One more question for this but I started to empty those jars out and so far I haven't seen anything in the three that I emptied that suggested or looked like contaminates/endospores that might have been growing. They still had some moisture but not a ton. They basically came out solid but crumbly, this is the only way I can explain it.  So is there anything in particular that I might not normally notice that could be indicative of contaminates? I can take some pictures if needed but I am not sure what good that will do.

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OfflinePsilicon
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Re: Question about inoculating jars that did not grow after inoculating once [Re: Cloner]
    #20609108 - 09/23/14 10:54 PM (10 years, 3 months ago)

Yep.  It's always the smell that tips me off.  Sniff every jar you open, always, unless they're obviously moldy and sporulated all to shit.  It's your best clue when something's off.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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OfflineCloner
The Cloner (eventually)
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Re: Question about inoculating jars that did not grow after inoculating once [Re: Psilicon]
    #20612327 - 09/24/14 04:06 PM (10 years, 3 months ago)

Ah ok. I know all of those jars I pitched yesterday did not smell out of place. They smelled like they did when I first put the stuff in so who knows. I am happy though because I finally have some myc growth in one of my new jars with the amazon strain.  So at least I know I am doing something right. Thanks again.

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