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Invisiblefungusflip
visionary

Registered: 12/08/02
Posts: 284
Loc: texas
overkill???
    #2061146 - 10/31/03 06:00 PM (13 years, 2 months ago)

i used 3 tablespoons of honey with 600mL water, same ratio with some karo too. which makes the percentage of honey/karo to water higher than 4%. my question is will it still work or is it absolutely neccessary to have the ratios perfect for spores to germinate or mycelium innocculant to colonize?


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Invisiblemicro
bunbun has a gungun
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: overkill??? [Re: fungusflip]
    #2061244 - 10/31/03 06:46 PM (13 years, 2 months ago)

Well, I wouldn't think that would put so much osmotic stress on the fungus as to kill it, but the problem is with that much carbon (sugars are very high in carbons -- the honey will have some nitrogen in the proteins, but a lot of these will denature during sterilization, anyway, and fold up, become insoluble in water and precipitate out) waste products will accumulate really quickly, and the fungus will die at that point.

I'm sure you'll get some growth, but it may stop early. If you do get a sufficient amount of mycelium I'd use it pretty soon....

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Micro


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Invisiblefungusflip
visionary

Registered: 12/08/02
Posts: 284
Loc: texas
Re: overkill??? [Re: micro]
    #2061530 - 10/31/03 09:05 PM (13 years, 2 months ago)

Quote:

micro said:
Well, I wouldn't think that would put so much osmotic stress on the fungus as to kill it, but the problem is with that much carbon (sugars are very high in carbons -- the honey will have some nitrogen in the proteins, but a lot of these will denature during sterilization, anyway, and fold up, become insoluble in water and precipitate out) waste products will accumulate really quickly, and the fungus will die at that point.

I'm sure you'll get some growth, but it may stop early. If you do get a sufficient amount of mycelium I'd use it pretty soon....
thanx for the info micro. i'm glad there are people here who know their shit. peace
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Micro




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let it be know, there is a fountain that was not made by the hands of men.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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