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OfflineBigButtMinaj
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Contamination Questions
    #20606464 - 09/23/14 02:36 PM (10 years, 3 months ago)

I have inoculated 24 jars and i feel that every single one has become contaminated. what could be the cause? should i have all the jars this close to each other? is it because my pressure cooker only goes up to 12psi?





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OfflinecronicrMFacebook
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Re: Contamination Questions [Re: BigButtMinaj]
    #20606476 - 09/23/14 02:39 PM (10 years, 3 months ago)

looks to me like your not using your pc right, purple mold i've only seen from unsterilized grains. make sure to vent your pc for a few minutes before putting the weight on. 12 psi should easily do it too btw


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I'm tired do me a favor

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InvisibleSanguin3
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Re: Contamination Questions *DELETED* [Re: cronicr]
    #20606602 - 09/23/14 03:13 PM (10 years, 3 months ago)

Post deleted by Sanguin3

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OfflineStevolindo
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Re: Contamination Questions [Re: Sanguin3]
    #20608238 - 09/23/14 07:53 PM (10 years, 3 months ago)

Is this a danger ? I hate canning all the little mycelia babies! Ugh! I need a glove box. Anyone know where I can find a cheep but a good one?


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InvisiblebodhisattaMDiscordReddit
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Re: Contamination Questions [Re: Stevolindo]
    #20608262 - 09/23/14 08:00 PM (10 years, 3 months ago)

not a glove box for this hobby a still air box. a box with the gloves not attached. They create suction and air currents you don't want that.

Look up SAB teks. You make one yourself for like 10$

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OfflineStevolindo
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Re: Contaminants [Re: bodhisatta]
    #20608295 - 09/23/14 08:11 PM (10 years, 3 months ago)

Thank you:thumbup: I will look into it now. I have a week left before I birth my latest cakes. And inoculating them with my spores. Can't wait to be a pro.


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InvisiblebodhisattaMDiscordReddit
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Re: Contaminants [Re: Stevolindo]
    #20608320 - 09/23/14 08:15 PM (10 years, 3 months ago)


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OfflineBigButtMinaj
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Re: Contamination Questions [Re: bodhisatta]
    #20608528 - 09/23/14 09:01 PM (10 years, 3 months ago)

Quote:

cronicr said:
looks to me like your not using your pc right, purple mold i've only seen from unsterilized grains. make sure to vent your pc for a few minutes before putting the weight on. 12 psi should easily do it too btw




Does it matter if the jars touch the water just a little bit? Also if I don't put the weight on when i first turn on the stove should the jars be in there?

Quote:

Sanguin3 said:
12 psi is fine you just need to run it a bit longer. How long are you pcing for?



An hour


Quote:

bodhisatta said:
not a glove box for this hobby a still air box. a box with the gloves not attached. They create suction and air currents you don't want that.

Look up SAB teks. You make one yourself for like 10$




Is it okay that I have all the jars next to each other as shown in the third picture in a cardboard box in a closet? If one jar was contaminated would it contaminate all the other ones?

Edited by BigButtMinaj (09/23/14 09:02 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: Contamination Questions [Re: BigButtMinaj]
    #20608582 - 09/23/14 09:13 PM (10 years, 3 months ago)

you put the jars in the PC close the PC take the weight or toggle off. let steam come out for a few minutes. put the weight or toggle on. let it build pressure. dial back the heat to maintain pressure.
the jars can touch the water but don't have the water more than an inch up the side of the jar. an hour is plenty enough time for a PF cake. all the jars should be out of a box light is good during colonization. They can be next to each other. Every bit of surface and air around you is full of thousands of contaminant spores and bacteria. Your jars don't get infected from that if they're built right so for the same reason they don't get infected by their neighbors. contamination doesn't magically jump from jar to jar. the spores of the contamination have to get to the brown rice flower of the PF cake. The dry vermiculite layer on the top keeps this from happening.

I would read most of the links in that last link I posted
and I would read the first link in my signature too

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OfflineKizzle
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Re: Contamination Questions [Re: BigButtMinaj]
    #20608595 - 09/23/14 09:18 PM (10 years, 3 months ago)

That purple mold is Fusarium. Whoever made the syringe probably was most likely preparing grain nearby where they were making spore prints.


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OfflineBigButtMinaj
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Re: Contamination Questions [Re: Kizzle]
    #20608630 - 09/23/14 09:24 PM (10 years, 3 months ago)

alright thanks I'm just surprised that ALL 24 jars which was like 3 spore syringes just got completely fucked. guess ill try again. i got one syringe left :/

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InvisiblebodhisattaMDiscordReddit
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Re: Contamination Questions [Re: BigButtMinaj]
    #20608645 - 09/23/14 09:27 PM (10 years, 3 months ago)

Did you order from a sponsor? or some other place?

You wipe needles with alcohol THEN flame them to red hot. Flame between each jar.

1cc per jar 0.25cc per hole or less.

you are using a dry vermiculite barrier right? BRF cake mix into half pint wide mouth jar then a layer of vermiculite over the top then the lid. No compacting.

Remove foil after the sterilization process don't leave the foil on the lids of your jars.

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OfflineBigButtMinaj
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Re: Contamination Questions [Re: bodhisatta]
    #20608655 - 09/23/14 09:30 PM (10 years, 3 months ago)

uhmm i use half pint wide mouth jars. alcohol, then flame sterilize, i did fuck up because i only inoculated through one hole but yes i have the dry verm layer and after sterilization and inoculation i left the foil off because people told me to do so. I got the spores from sporebank.

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InvisiblebodhisattaMDiscordReddit
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Re: Contamination Questions [Re: BigButtMinaj]
    #20608663 - 09/23/14 09:32 PM (10 years, 3 months ago)

then yea I would point to the syringes just being bad. you did everything else right.

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InvisibleSanguin3
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Re: Contamination Questions *DELETED* [Re: bodhisatta]
    #20608675 - 09/23/14 09:34 PM (10 years, 3 months ago)

Post deleted by Sanguin3

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OfflineBigButtMinaj
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Re: Contamination Questions [Re: Sanguin3]
    #20608677 - 09/23/14 09:35 PM (10 years, 3 months ago)

is ralphsters any good?

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InvisiblebodhisattaMDiscordReddit
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Re: Contamination Questions [Re: BigButtMinaj]
    #20608691 - 09/23/14 09:37 PM (10 years, 3 months ago)

for microscopy only :thumbup:

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InvisibleGhatti
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Re: Contamination Questions [Re: bodhisatta]
    #20608814 - 09/23/14 10:03 PM (10 years, 3 months ago)

Why have water no more than an inch up the jars?

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InvisiblebodhisattaMDiscordReddit
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Re: Contamination Questions [Re: Ghatti]
    #20608851 - 09/23/14 10:07 PM (10 years, 3 months ago)

superheated steam is the heat transfer mechanism it carries more energy.

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InvisibleGhatti
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Re: Contamination Questions [Re: bodhisatta]
    #20608912 - 09/23/14 10:17 PM (10 years, 3 months ago)

Understood. So what temp does the water itself under pressure get to? The steam and water are at 2 different temps?

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Kraken Kratom Red Vein Kratom   Unfolding Nature Unfolding Nature: Being in the Implicate Order


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