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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Question about sterilizing a syringe
#20605050 - 09/23/14 07:17 AM (10 years, 3 months ago) |
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So when you pc a syringe, them take it to your sab to use it, once you draw the plunger back aren't you basically introducing any contamination that has landed behind the plunger into the barrel? I doubt the plunged makes that good of a seal as to not ride over the top of spores etc.
I know to do all this as cleanly as possible but am I right?
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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 8 years, 1 month
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Re: Question about sterilizing a syringe [Re: Ghatti]
#20605091 - 09/23/14 07:35 AM (10 years, 3 months ago) |
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Gonna piggyback here OP if you don't mind.
I wrapped two syringes filled with water in tinfoil and pc'd them for an hour at 15psi along with some agar plates. When I unwrapped the tinfoil couple days later there was only a small amount of water.???
How'd that happen?
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 9 hours, 46 minutes
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Re: Question about sterilizing a syringe [Re: Ghatti]
#20605095 - 09/23/14 07:37 AM (10 years, 3 months ago) |
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take it out the tin foil in the sab
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Question about sterilizing a syringe [Re: tripdawg420]
#20605124 - 09/23/14 08:00 AM (10 years, 3 months ago) |
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The water basically boils out of the syringe, always happens. I know take em out in a sab etc etc. Wasn't my question really.
In the hospital for instance doesn't the same apply when a nurse draws up a shot? I know its different because humans have immune systems and all but that's not the question.
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 11 months, 19 days
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Re: Question about sterilizing a syringe [Re: tripdawg420]
#20605127 - 09/23/14 08:03 AM (10 years, 3 months ago) |
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I am not recommeneding this, so do at own risk if you decide to try it. I will tell you what I have done but is probably not the best way. I found it on an actual tek on here and it has worked for me everytime however. I would not consider it proper sterilization technique simply because the short time involved. I can't remember the name of tek but it says to get water to a rolling boil in a small pan on stove. Draw boiling water into syringe, squirt out into sink/towel. Repeat this 2-3 times. On the last draw flame the tip red hot and replace needle cap. Then it says to allow it to cool slowly at room temp. I have done this time and again and so far it has worked fine. I am sure someone will say not to do this, and they know much more than me. I have also taken prints in a new ziplock snack bag and has worked as well. I always use the syringes right away (within 2 days) and don't store long term. Maybe that is why it works and wouldn't work if I tried storing them for a few weeks? Am actually interested in what some of the experienced people think about this. I was leary when I first read it as well, but shit, it's super easy and so far has worked fine.
PS - Again, not telling anyone to do this unless they want to experiment. Just sharing an experience from a decision I decided to make based on a tek.
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin
Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
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Mdahmer
Aloysius devadander abercrombie



Registered: 04/05/14
Posts: 2,516
Last seen: 7 years, 5 months
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Re: Question about sterilizing a syringe [Re: FreeWorldOrder]
#20605131 - 09/23/14 08:06 AM (10 years, 3 months ago) |
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I've done the boiling water with LCs and it worked just fine. Suck it up a few times ande expel into the sink each time, on the last time leave water in like free said, if you wanna make a syringe you have sterile water, for LC just shoot the water out into something in your SAB, slowly, then draw your LC in. I don't use syringes often but that's how I did it
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LogicalAnswer
Wizard



Registered: 06/25/14
Posts: 26
Last seen: 8 years, 5 months
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Re: Question about sterilizing a syringe [Re: Mdahmer]
#20605218 - 09/23/14 08:53 AM (10 years, 3 months ago) |
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I'd like to see some more detailed answers here, because people go to all this trouble to PC the grains and use flow hoods for G2G, and all these sterile teks... but making syringes seems to be a hole in the process. I'm not sure any of the teks I've read are exactly "sterile". If it's so important to have everything going into the SAB for inoculation so sterile, the same has to be true of your spore print and syringe making processes. I guess none of us will really have the resources to be truly sterile like a pharmaceutical lab or something. I guess it's just about minimizing opportunity for contamination. Taking a foil wrapped, newly PC'd syringe into your SAB, wiping it down good with alcohol, then unwrapping it is eliminating the risk about as much as you can? I'd love to see some more input on this one.
-------------------- My journey from user to enthusiast to finder to hunter to stockpiler to now cultivator has led me here.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Question about sterilizing a syringe [Re: LogicalAnswer]
#20605229 - 09/23/14 08:58 AM (10 years, 3 months ago) |
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That's about it in a nutshell. In a pharmaceutical setting for pre filled syringes or dosage syringes, syringes are unwrapped in the hood, the top of vials are wiped with IPA and sometimes sodium hypochlorite aka bleach and then drawn up and capped.
This is only for prepared doses and says nothing about anything drawn up at bedside in a rush.
As far as sterile prints there are a few ways to go about it but most are a pia.
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Mdahmer
Aloysius devadander abercrombie



Registered: 04/05/14
Posts: 2,516
Last seen: 7 years, 5 months
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Re: Question about sterilizing a syringe [Re: LogicalAnswer]
#20605236 - 09/23/14 09:00 AM (10 years, 3 months ago) |
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Most people that I've seen don't use syringes for long is why I think there's not as much info on them. I've personally never made a spore syringe. Once you get into agar spore syringes become unnecessary. But there are teks out there but like you said they may be old and outdated.
Sterilizing the needle with boiling water then wiping the outside with alcohol should be ok but PC he whole thing if you have more time and wanna be safer. After that I couldn't tell you what to do lol
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bloodyice69
GrassHopper



Registered: 08/06/14
Posts: 296
Loc: Colonizing Jars
Last seen: 4 years, 2 months
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Re: Question about sterilizing a syringe [Re: FreeWorldOrder]
#20605243 - 09/23/14 09:04 AM (10 years, 3 months ago) |
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Quote:
FreeWorldOrder said: I am not recommeneding this, so do at own risk if you decide to try it. I will tell you what I have done but is probably not the best way. I found it on an actual tek on here and it has worked for me everytime however. I would not consider it proper sterilization technique simply because the short time involved. I can't remember the name of tek but it says to get water to a rolling boil in a small pan on stove. Draw boiling water into syringe, squirt out into sink/towel. Repeat this 2-3 times. On the last draw flame the tip red hot and replace needle cap. Then it says to allow it to cool slowly at room temp. I have done this time and again and so far it has worked fine. I am sure someone will say not to do this, and they know much more than me. I have also taken prints in a new ziplock snack bag and has worked as well. I always use the syringes right away (within 2 days) and don't store long term. Maybe that is why it works and wouldn't work if I tried storing them for a few weeks? Am actually interested in what some of the experienced people think about this. I was leary when I first read it as well, but shit, it's super easy and so far has worked fine.
PS - Again, not telling anyone to do this unless they want to experiment. Just sharing an experience from a decision I decided to make based on a tek.
i read that same tek. cant remember where tho. but i wrote it down at the time.
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weaksause
Stranger

Registered: 08/26/14
Posts: 254
Last seen: 10 years, 9 days
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Re: Question about sterilizing a syringe [Re: bloodyice69]
#20605267 - 09/23/14 09:14 AM (10 years, 3 months ago) |
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Quote:
bloodyice69 said:
Quote:
FreeWorldOrder said: I am not recommeneding this, so do at own risk if you decide to try it. I will tell you what I have done but is probably not the best way. I found it on an actual tek on here and it has worked for me everytime however. I would not consider it proper sterilization technique simply because the short time involved. I can't remember the name of tek but it says to get water to a rolling boil in a small pan on stove. Draw boiling water into syringe, squirt out into sink/towel. Repeat this 2-3 times. On the last draw flame the tip red hot and replace needle cap. Then it says to allow it to cool slowly at room temp. I have done this time and again and so far it has worked fine. I am sure someone will say not to do this, and they know much more than me. I have also taken prints in a new ziplock snack bag and has worked as well. I always use the syringes right away (within 2 days) and don't store long term. Maybe that is why it works and wouldn't work if I tried storing them for a few weeks? Am actually interested in what some of the experienced people think about this. I was leary when I first read it as well, but shit, it's super easy and so far has worked fine.
PS - Again, not telling anyone to do this unless they want to experiment. Just sharing an experience from a decision I decided to make based on a tek.
i read that same tek. cant remember where tho. but i wrote it down at the time.
me too it's a solid tek , never had troubles flame needle / chemical sterlize outside / boil water coold inside
made sure not to recommend it, so i dont want too either , but it works good with me . maybe someone with more understand9ing can recommend it to all, then i'll do the same , lol
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supernewfie
some fella


Registered: 09/24/13
Posts: 1,103
Loc: Canada
Last seen: 6 years, 4 months
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Re: Question about sterilizing a syringe [Re: weaksause]
#20605383 - 09/23/14 10:07 AM (10 years, 3 months ago) |
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boil the syringe in a pot of water with a lid(and a shot glass if youre making aspore syringe from print)... after 10 or 15 mins take it (and the shot glass, wrap in fresh ziplock bag) out and suck some water up out of the pot then wrap it up.... flame the needle before putting in sab to make the spore syringe. have the spores scraped into the shot glass and cover with saran. poke needle through saran onto the bottom of shot glass and empty and suck the water back up a few times(after the water has cooled)to disperse spores evenly throughout the syringe
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FreeWorldOrder

Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 11 months, 19 days
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Re: Question about sterilizing a syringe [Re: supernewfie]
#20606376 - 09/23/14 02:14 PM (10 years, 3 months ago) |
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Quote:
supernewfie said: boil the syringe in a pot of water with a lid(and a shot glass if youre making aspore syringe from print)... after 10 or 15 mins take it (and the shot glass, wrap in fresh ziplock bag) out and suck some water up out of the pot then wrap it up.... flame the needle before putting in sab to make the spore syringe. have the spores scraped into the shot glass and cover with saran. poke needle through saran onto the bottom of shot glass and empty and suck the water back up a few times(after the water has cooled)to disperse spores evenly throughout the syringe
yup... pretty much the same concept I use, using the ziplocks with the print on the inside corner. Seems to work well. But like Mdahmer was stating, most people who continue on in this hobby will move on to agar. Then you can do isolates, slants, and all that good stuff... and know your transfers are good...
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin
Lets Grow Mushrooms Videos
PastyWhyte's Easy Agar TEK
Agar's Liquid Inoculant TEK
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