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InvisibleGhatti
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4% lme lc
    #20605167 - 09/23/14 08:23 AM (10 years, 3 months ago)

Looking for an old LC recipe. Super simple one just water and LME. Although I don't remember how to calculate to 4% since my water is obviously liquid and my LME is a solid.

Can anyone help for a 500ml recipe?

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InvisibleGhatti
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Re: 4% lme lc [Re: Ghatti]
    #20605182 - 09/23/14 08:30 AM (10 years, 3 months ago)

Fuck it Imma do 0.8g per 500ml per this thread

http://www.shroomery.org/forums/showflat.php/Number/16166162#16166162

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OfflineMdahmer
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Re: 4% lme lc [Re: Ghatti]
    #20605193 - 09/23/14 08:38 AM (10 years, 3 months ago)

I used just LME I think I did a teaspoon in a qt jar, not very scientific lol but it worked fine although I just did it the once

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InvisiblePastywhyteMDiscord
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Re: 4% lme lc [Re: Mdahmer]
    #20605248 - 09/23/14 09:05 AM (10 years, 3 months ago)

If you use meteric the volume of water corrolates to the weight (1 liter of water weighs a kilo). So 2% of 1 liter (1000ml) would be 20grams.

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InvisibleGhatti
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Re: 4% lme lc [Re: Pastywhyte]
    #20605265 - 09/23/14 09:14 AM (10 years, 3 months ago)

Lol 10g is what I use for my plates per 500ml. Guess that makes sense though. If so how does TL get away with such a low ratio and still get good growth.

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OfflineChk
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Re: 4% lme lc [Re: Ghatti]
    #20605342 - 09/23/14 09:48 AM (10 years, 3 months ago)

I guess it's the same than for agar, the less nutrient you have the faster you mycelium expands.


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InvisiblePastywhyteMDiscord
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Re: 4% lme lc [Re: Chk]
    #20605369 - 09/23/14 10:02 AM (10 years, 3 months ago)

You want enough to sustain the colony but not so much that growth slows too much. 2% is a good number to start with however its not in stone. There was an experiment a while back where a certain monoculture performed best at 6%.

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Offlineblojo02184
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Re: 4% lme lc [Re: Pastywhyte]
    #20605996 - 09/23/14 12:52 PM (10 years, 3 months ago)

Yeah, I've read anywhere between 2-8% is good.
the lower the percentage, the more rhizo. the growth looks.

same goes for plates.
I make rich plates to sustain a culture, but when isolating I make a very weak batch to transfer to. this in turn make the mycelium race against itself in search of nutrients.

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