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OhMycGod
Wandering Mystic

Registered: 09/20/14
Posts: 18
Loc: who's ear country
Last seen: 9 years, 8 months
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No doubt it's a contam. But how Q.Q
#20605261 - 09/23/14 09:12 AM (10 years, 3 months ago) |
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Lost several jars today to a green invader 
Standard PF tek ideas, brf - shoulderless jars- boil for 90minutes
did my dry layer, went through the noise of building a SAB after loosing a full first round (to an improper boil, but bathtub SAB probably wasn't helping)
Anyway, my green pest is directly under the dry verm layer(at least that's where it starts), but I'm wondering if I'm just pushing the dry layer aside when I put the needle in. It's directly under one of the inoculation points on all 3 (I guess it could be a hitch hiker but with a proper boil, sprayed SAB with h2o/70 iso, gloves, hair tie, breathing mask...thing)
Gonna grab some batteries for the camera but not sure how much it would help. Thanks for any help in advance
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental.
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dpomalia
Strange


Registered: 11/06/13
Posts: 986
Last seen: 9 years, 4 days
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Re: No doubt it's a contam. But how Q.Q [Re: OhMycGod]
#20605275 - 09/23/14 09:19 AM (10 years, 3 months ago) |
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Probably too much solution. How many ccs per hole?
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OhMycGod
Wandering Mystic


Registered: 09/20/14
Posts: 18
Loc: who's ear country
Last seen: 9 years, 8 months
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Re: No doubt it's a contam. But how Q.Q [Re: dpomalia]
#20605285 - 09/23/14 09:23 AM (10 years, 3 months ago) |
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1/4 cc per hole - 1/2 inc dry verm layer (to specify)
maybe baking the verm? read about it but didn't check the date so not sure if that's been proven useless/outdated or....
edit: also to note, I'm not such a noob that I'm inoc. the dry layer. I make sure the needle is in the substrate before I apply any pressure to the plunger >.>
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental.
Edited by OhMycGod (09/23/14 09:25 AM)
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
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Re: No doubt it's a contam. But how Q.Q [Re: OhMycGod]
#20606968 - 09/23/14 04:08 PM (10 years, 3 months ago) |
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Most commonly mold in PF jars is from a contaminated syringe or because the dry verm layer wasn't effective, like if was too thin, got wet, was jostled around, or was made with coarse vermiculite instead of fine.
It's certainly possible to push contaminants on the dry verm layer into the jar with the needle if the holes were left uncovered before inoculation. Since you're seeing it under separate inoculation points though I suspect it's the syringe.
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OhMycGod
Wandering Mystic


Registered: 09/20/14
Posts: 18
Loc: who's ear country
Last seen: 9 years, 8 months
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Re: No doubt it's a contam. But how Q.Q [Re: Kizzle]
#20607293 - 09/23/14 04:58 PM (10 years, 3 months ago) |
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Then I can hopefully rest as ease, as these were free from a local friend (and I later found they were supplied by some random www). Ordered some from a sponsor, expecting them in a few days. May invest in some micro pore tape just to be safe. Thanks ^.^
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: No doubt it's a contam. But how Q.Q [Re: OhMycGod]
#20611510 - 09/24/14 01:30 PM (10 years, 3 months ago) |
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Quote:
Ordered some from a sponsor
That's moving in the right direction. 
Quote:
May invest in some micro pore tape just to be safe.
Great idea.
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