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Amazon Shop for: ½ Pint Jars, Agar, Parafilm, Wild Bird Seed

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OfflineSuntzu
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Registered: 10/15/99
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Panellus stipticus
    #2060470 - 10/31/03 02:14 PM (13 years, 1 month ago)

Hey all, my garden finally started putting out a few Panellus clusters.  When they were young, the glowing could be seen even with some street lighting nearby.  The grain jars are still glowing like mad.  Despite this, the damn things will not show up on my digital camera, even with maximum aperture and several seconds exposure time.  I really don't know what I'm doing photographically, so there could be some operator error.  Anyway, the glowing of this cluster was beginning to weaken. . .it had been on the log for a couple of weeks.  I cut it off, took it to work, and used a chemiluminescent imager that we use for incredibly weak signals from things like western blots.  It's not a cool color picture like I've seen before, but I thought it was pretty cool.    The imaging box is specifically designed to be absolutely light-proof, everything you see in the black and white photo is coming from the fungus :smile:

I have another younger cluster that is coming up.  I'm going to get an image of that one, along with a year-old grain jar that is still glowing like crazy [and pinning, surprisingly].

Happy Halloween!


 


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OfflineVividDreams
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Re: Panellus stictipus [Re: Suntzu]
    #2062365 - 11/01/03 08:39 AM (13 years, 1 month ago)

Very very Cool..Hope you figure out the camera deal. I'd really like to see pictures of this! :smile: 


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Invisiblearmedia
Dikaryon

Registered: 11/03/01
Posts: 169
Re: Panellus stictipus [Re: Suntzu]
    #2062573 - 11/01/03 01:30 PM (13 years, 1 month ago)

Suntzu -

Would you care to share your substrate recipe for these?

- A


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OfflineSuntzu
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Re: Panellus stictipus [Re: armedia]
    #2062668 - 11/01/03 02:38 PM (13 years, 1 month ago)

Nothing really special, alder logs plugged heavily with dowels, buried. . .
Only 2-3 of these logs were Panellus, the others are reishi and P. pulm. . .





I'm pretty sure the culture was from you, armedia.


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Invisiblearmedia
Dikaryon

Registered: 11/03/01
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Re: Panellus stictipus [Re: Suntzu]
    #2065016 - 11/02/03 03:04 PM (13 years, 1 month ago)

Cool. Now that I have my garden settled, I'll plug some oak logs next spring.

As for your year old jar: six months is my record. I have a pet theory that the H2O2 I always use in my cultures, as it breaks down, provides additional O2 to the redox reaction that is the source of the bioluminescence, allowing maximal brightness.

Glad to hear my culture is spreading far and wide.

- A


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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Panellus stictipus [Re: Suntzu]
    #2069844 - 11/04/03 11:02 AM (13 years, 1 month ago)

omg!

He did it ! He did it!
:heart: 


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OfflineSuntzu
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Re: Panellus stictipus [Re: Suntzu]
    #2070166 - 11/04/03 05:29 PM (13 years, 1 month ago)

People were looking at me wierd when they saw me sticking a jar in the imager :wink:

This champion of a jar is just under ONE YEAR old.  The pins are a couple of months old.  If you can get someone to shut themselves in your closet, these Panellus jars are great to share with myco-newbies.  Oh, and I was wrong about it being a grain jar, it's actually mostly dowels [innoculated with birdseed].



Here's the 'more fresh' cluster.  You can see how this one was much brighter, it was even illuminating the paper towel it was sitting on.




 


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OfflineSuntzu
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Re: Panellus stictipus [Re: Suntzu]
    #2070213 - 11/04/03 05:45 PM (13 years, 1 month ago)

For anyone interested in more information about the bioluminescence pathway, this is a short paper on the subject:

http://www.ecol.kvl.dk/~sto/PDFposters/OlssonRedox2000.PDF

The practical thing I took from this paper is to let the jar incubate fairly well-sealed for a few days, then loosen the lids before 'viewing'. It really works, and only takes a couple of minutes for the increased luminescence to occur. The same principle holds for agar cultures. . .keep sealed in parafilm until ready to 'view', then remove the film.


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Offliner05c03
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Re: Panellus stictipus [Re: Suntzu]
    #2071054 - 11/04/03 10:00 PM (13 years, 1 month ago)

How long from plates to fruits?


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Offlinezeronio
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Re: Panellus stictipus [Re: Suntzu]
    #2072421 - 11/05/03 07:56 AM (13 years, 1 month ago)

Wow, that looks great!


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OfflineISH
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Re: Panellus stictipus [Re: zeronio]
    #2091204 - 11/11/03 03:11 AM (13 years, 25 days ago)

WOW, That's some awesome pics.

ISH


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I think of going to the grave without having a psychedelic experience, like going to the grave without having sex. It means that you never figured out what it was all about. The mystery is in the body, and the way the body works itself into nature.
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OfflineFungushead
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Re: Panellus stictipus [Re: ISH]
    #2401534 - 03/04/04 08:08 AM (12 years, 8 months ago)

Why does that picture make me laugh so? It's so...appropriately innapropriate.

:lol:


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Invisiblephobey
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Registered: 03/21/03
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Loc: home
Re: Panellus stictipus [Re: Suntzu]
    #2405621 - 03/05/04 04:11 AM (12 years, 8 months ago)

Awesome bet they make a nice trip toy


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Amazon Shop for: ½ Pint Jars, Agar, Parafilm, Wild Bird Seed

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