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Offlineaww
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Registered: 07/04/14
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How much spores per syringe?
    #20600789 - 09/22/14 11:43 AM (10 years, 3 months ago)

Hi

I am going to make my first spore syringe. I have a 20ml syringe and a 1" diameter print. Should I use the whole print for the syringe?

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Offlineant707
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Re: How much spores per syringe? [Re: aww]
    #20600800 - 09/22/14 11:46 AM (10 years, 3 months ago)

no, you should not use the whole print.

use only a small amount.

a little goes a long way. check out this tek Stro's Spore Syringe

Edited by ant707 (09/22/14 11:49 AM)

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Offlineaww
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Re: How much spores per syringe? [Re: aww]
    #20600972 - 09/22/14 12:39 PM (10 years, 3 months ago)

Thanks, is 1/8 good?

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InvisibleThadeous
On the path

Registered: 08/02/11
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Re: How much spores per syringe? [Re: aww]
    #20600989 - 09/22/14 12:43 PM (10 years, 3 months ago)

See the scraped portion in this image? [taken from the link above]

About that much.  :smile:

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Offlineaww
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Re: How much spores per syringe? [Re: aww]
    #20601025 - 09/22/14 12:51 PM (10 years, 3 months ago)

Oh, not more? Then my little print can make a lot of syringes :smile:

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OfflinePsyDragon
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Re: How much spores per syringe? [Re: aww]
    #20601036 - 09/22/14 12:53 PM (10 years, 3 months ago)

LOL, so I guess I shouldn't have made a 12cc syringe from an entire 1" print the other day. The syringe water looks purple. :laugh:

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Offlineaww
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Re: How much spores per syringe? [Re: aww]
    #20602275 - 09/22/14 05:18 PM (10 years, 3 months ago)

Now I'm just done making my first syringe! I hope my sterilization tek was up to par.

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Offlineant707
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Re: How much spores per syringe? [Re: aww]
    #20602345 - 09/22/14 05:34 PM (10 years, 3 months ago)

:goodluck:

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OfflinePussyFart
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Re: How much spores per syringe? [Re: ant707]
    #20602365 - 09/22/14 05:38 PM (10 years, 3 months ago)

I use even less than that.......prints can make a ton of syringes, and even more agar dishes.

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Offlineant707
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Re: How much spores per syringe? [Re: PussyFart]
    #20602378 - 09/22/14 05:40 PM (10 years, 3 months ago)

Quote:

PussyFart said:
I use even less than that.......prints can make a ton of syringes, and even more agar dishes.




how do you put your spores to agar PF?

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OfflinePussyFart
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Re: How much spores per syringe? [Re: ant707]
    #20602404 - 09/22/14 05:45 PM (10 years, 3 months ago)

Heat up an inoculation loop to red hot, then cool it down in an agar plate, creating a small puddle in the center of the dish.

Then I scrape some spores with the loop, then touch the center of the agar plate with it, wiping in a circular motion, then seal up the dish with parafilm or 1" strips of saran wrap.

That is all.....then you just wait a week or so for growth and make some transfers.

All of this is done in a SAB(Still Air Box) of course.

Edited by PussyFart (09/22/14 05:46 PM)

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Offlineaww
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Re: How much spores per syringe? [Re: aww]
    #20602405 - 09/22/14 05:45 PM (10 years, 3 months ago)

Is there a easy way to check if my syringe contains contamination?

Edited by PussyFart (09/22/14 05:49 PM)

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Offlineant707
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Registered: 07/26/14
Posts: 579
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Re: How much spores per syringe? [Re: aww]
    #20602425 - 09/22/14 05:48 PM (10 years, 3 months ago)

Quote:

PussyFart said:
Heat up an inoculation loop to red hot, then cool it down in an agar plate, creating a small puddle in the center of the dish.

Then I scrape some spores with the loop, then touch the center of the agar plate with it, wiping in a circular motion, then seal up the dish with parafilm or 1" strips of saran wrap.

That is all.....then you just wait a week or so for growth and make some transfers.

All of this is done in a SAB(Still Air Box) of course.





cool. i recently got some one-time use cotton swabs to try swiping some spores on a plate.
i need to invest in an inoculation loop

Quote:

aww said:
Is there a easy way to check if my syringe contains contamination?




agar! :wink:

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OfflinePussyFart
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Re: How much spores per syringe? [Re: aww]
    #20602429 - 09/22/14 05:49 PM (10 years, 3 months ago)

Quote:

aww said:
Is there a easy way to check if my syringe contains contamination?




Yes, inoculate something.

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InvisibleStropharis
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Re: How much spores per syringe? [Re: Thadeous]
    #20602612 - 09/22/14 06:17 PM (10 years, 3 months ago)

Quote:

Thadeous said:
See the scraped portion in this image? [taken from the link above]

About that much.  :smile:




I was being generous, a little does indeed go a very long way. A fraction of that would suffice.

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OfflinePurePleasure
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Registered: 08/29/14
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Re: How much spores per syringe? [Re: PussyFart]
    #20602866 - 09/22/14 07:00 PM (10 years, 3 months ago)

Quote:

PussyFart said:
Heat up an inoculation loop to red hot, then cool it down in an agar plate, creating a small puddle in the center of the dish.

Then I scrape some spores with the loop, then touch the center of the agar plate with it, wiping in a circular motion, then seal up the dish with parafilm or 1" strips of saran wrap.

That is all.....then you just wait a week or so for growth and make some transfers.

All of this is done in a SAB(Still Air Box) of course.




:takingnotes:


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)



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OfflinePurePleasure
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Registered: 08/29/14
Posts: 5,893
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Re: How much spores per syringe? [Re: PussyFart]
    #20602874 - 09/22/14 07:01 PM (10 years, 3 months ago)

Quote:

PussyFart said:
Quote:

aww said:
Is there a easy way to check if my syringe contains contamination?




Yes, inoculate something.



:rofl:


--------------------
If you aren't happy with what you have now, how could you be any happier with more?  (Get your SGFC high.)



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Offlineaww
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Re: How much spores per syringe? [Re: aww]
    #20603051 - 09/22/14 07:30 PM (10 years, 3 months ago)

I will inoculate 6 glasses tomorrow :smile: Hopefully it will turn out fine.

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