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pepperpotpan
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Registered: 11/13/13
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substrate and filterbags
#20601871 - 09/22/14 04:05 PM (10 years, 3 months ago) |
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Hi all so I got sum filter bags and want to bulk substrate in them. inoculate after pasteurizing with sum spawn. Colonize it in the bag and fruit it when done is that correct? And what is a good substrate to use. I have straw, sawdust, bran, rye flour and gypsum can I mix it all together and use that as substrate its for cubes
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Pastywhyte
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Registered: 09/15/12
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Loc: Canada
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If your using grains or flour in the mix it will need to be sterilized and you will need to have grains or flour in the mix.
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pepperpotpan
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Re: substrate and filterbags [Re: Pastywhyte]
#20601916 - 09/22/14 04:18 PM (10 years, 3 months ago) |
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ok thank so ill just pressure cook the substrate for 1.5h and inoculate in glove box
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azur
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Registered: 04/21/12
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Sawdust is unnecessary. And if you pc straw, your bags will go green!
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pepperpotpan
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Re: substrate and filterbags [Re: azur]
#20601964 - 09/22/14 04:26 PM (10 years, 3 months ago) |
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should I just go with sum verm,coir,straw and gypsum and pasturize it instead
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azur
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If you are gonna knock it up with grain. I suggest you skip the straw till you've got a bit more experience
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pepperpotpan
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Re: substrate and filterbags [Re: azur]
#20602165 - 09/22/14 04:58 PM (10 years, 3 months ago) |
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ok thanks for the help
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Pastywhyte
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Registered: 09/15/12
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Re: substrate and filterbags [Re: azur]
#20602377 - 09/22/14 05:40 PM (10 years, 3 months ago) |
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Quote:
azur said: If you are gonna knock it up with grain.
I got the impression OP was planning to inoculate the bags with a syringe, which is why I suggested he needed grains or flour in the mix. Straw would be fine in that instance too.
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azur
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Re: substrate and filterbags [Re: Pastywhyte]
#20602463 - 09/22/14 05:56 PM (10 years, 3 months ago) |
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I would not sterilize straw!
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Pastywhyte
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Registered: 09/15/12
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Re: substrate and filterbags [Re: azur]
#20602529 - 09/22/14 06:07 PM (10 years, 3 months ago) |
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Even if its colonized in a filter patch bag? Just curious, I have never tried myself but it was something I was planning to try in the future. I figure if it is fully colonized in sterile filter patch bags then there should be no contam risk. Please elaborate.
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: substrate and filterbags [Re: Pastywhyte]
#20602649 - 09/22/14 06:22 PM (10 years, 3 months ago) |
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Quote:
Pastywhyte said: Even if its colonized in a filter patch bag? Just curious, I have never tried myself but it was something I was planning to try in the future. I figure if it is fully colonized in sterile filter patch bags then there should be no contam risk. Please elaborate.
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azur
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Re: substrate and filterbags [Re: MrGiraffe]
#20602795 - 09/22/14 06:48 PM (10 years, 3 months ago) |
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Straw needs to be pasteurized. Sterilizing straw is guarantee failure.
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Pastywhyte
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Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: substrate and filterbags [Re: azur]
#20602901 - 09/22/14 07:05 PM (10 years, 3 months ago) |
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Quote:
azur said: Straw needs to be pasteurized. Sterilizing straw is guarantee failure.
Don't tell me that. . .
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: substrate and filterbags [Re: azur]
#20602924 - 09/22/14 07:09 PM (10 years, 3 months ago) |
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Quote:
azur said: Straw needs to be pasteurized. Sterilizing straw is guarantee failure.
Spawning in open air sure. What's the problem with it being sterilized within a filter patch bag and inoculating it with grain spawn in a SAB or in front.of a hood?
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azur
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Re: substrate and filterbags [Re: MrGiraffe]
#20602971 - 09/22/14 07:16 PM (10 years, 3 months ago) |
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Idk.
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azur
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Registered: 04/21/12
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Loc: Daid
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Re: substrate and filterbags [Re: azur]
#20602997 - 09/22/14 07:21 PM (10 years, 3 months ago) |
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I still don't think i would do. Never tried though
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: substrate and filterbags [Re: azur]
#20603086 - 09/22/14 07:37 PM (10 years, 3 months ago) |
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The point of pasteurizing bulk poo or straw is to keep beneficial bacteria around to prevent contams from gaining a foothold before it can be colonized (especially if spawning in open air). In a sealed bag with a filter, it wouldn't be any different than anything else you put in a jar or bag through a PC run (grains etc). As long a sterile technique is used, there shouldn't be any more chance of a contam (at least in theory). Sounds like an experiment waiting to happen. Who knows maybe the heat of the PC run will break down the straw even more so than a pasteurization run, aiding in the mycelium's ability to digest it quicker.
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azur
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: substrate and filterbags [Re: MrGiraffe]
#20603198 - 09/22/14 08:01 PM (10 years, 3 months ago) |
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Yea. Sounds interesting. I might try it with an LI
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pepperpotpan
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Re: substrate and filterbags [Re: azur]
#20604394 - 09/22/14 11:43 PM (10 years, 3 months ago) |
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thanks every one so ill sterilize 3 bags with straw bran rye and ill pasteurize 3 bags with verm coir and straw and c what happens. ill post some pics of the bags later
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azur
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
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