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aww
Stranger


Registered: 07/04/14
Posts: 44
Last seen: 10 years, 2 months
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How much spores per syringe?
#20600789 - 09/22/14 11:43 AM (10 years, 3 months ago) |
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Hi
I am going to make my first spore syringe. I have a 20ml syringe and a 1" diameter print. Should I use the whole print for the syringe?
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ant707
not a puppet


Registered: 07/26/14
Posts: 579
Loc: northeast florida
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Re: How much spores per syringe? [Re: aww]
#20600800 - 09/22/14 11:46 AM (10 years, 3 months ago) |
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no, you should not use the whole print.
use only a small amount.
a little goes a long way. check out this tek Stro's Spore Syringe
Edited by ant707 (09/22/14 11:49 AM)
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aww
Stranger


Registered: 07/04/14
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Re: How much spores per syringe? [Re: aww]
#20600972 - 09/22/14 12:39 PM (10 years, 3 months ago) |
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Thanks, is 1/8 good?
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Thadeous
On the path

Registered: 08/02/11
Posts: 1,101
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Re: How much spores per syringe? [Re: aww]
#20600989 - 09/22/14 12:43 PM (10 years, 3 months ago) |
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See the scraped portion in this image? [taken from the link above] 
About that much.
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aww
Stranger


Registered: 07/04/14
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Re: How much spores per syringe? [Re: aww]
#20601025 - 09/22/14 12:51 PM (10 years, 3 months ago) |
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Oh, not more? Then my little print can make a lot of syringes
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PsyDragon
journeyman


Registered: 10/21/05
Posts: 627
Loc: state of perpetual transi...
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Re: How much spores per syringe? [Re: aww]
#20601036 - 09/22/14 12:53 PM (10 years, 3 months ago) |
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LOL, so I guess I shouldn't have made a 12cc syringe from an entire 1" print the other day. The syringe water looks purple.
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aww
Stranger


Registered: 07/04/14
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Re: How much spores per syringe? [Re: aww]
#20602275 - 09/22/14 05:18 PM (10 years, 3 months ago) |
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Now I'm just done making my first syringe! I hope my sterilization tek was up to par.
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ant707
not a puppet


Registered: 07/26/14
Posts: 579
Loc: northeast florida
Last seen: 7 years, 11 months
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Re: How much spores per syringe? [Re: aww]
#20602345 - 09/22/14 05:34 PM (10 years, 3 months ago) |
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: How much spores per syringe? [Re: ant707]
#20602365 - 09/22/14 05:38 PM (10 years, 3 months ago) |
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I use even less than that.......prints can make a ton of syringes, and even more agar dishes.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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ant707
not a puppet


Registered: 07/26/14
Posts: 579
Loc: northeast florida
Last seen: 7 years, 11 months
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Re: How much spores per syringe? [Re: PussyFart]
#20602378 - 09/22/14 05:40 PM (10 years, 3 months ago) |
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Quote:
PussyFart said: I use even less than that.......prints can make a ton of syringes, and even more agar dishes.
how do you put your spores to agar PF?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: How much spores per syringe? [Re: ant707]
#20602404 - 09/22/14 05:45 PM (10 years, 3 months ago) |
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Heat up an inoculation loop to red hot, then cool it down in an agar plate, creating a small puddle in the center of the dish.
Then I scrape some spores with the loop, then touch the center of the agar plate with it, wiping in a circular motion, then seal up the dish with parafilm or 1" strips of saran wrap.
That is all.....then you just wait a week or so for growth and make some transfers.
All of this is done in a SAB(Still Air Box) of course.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (09/22/14 05:46 PM)
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aww
Stranger


Registered: 07/04/14
Posts: 44
Last seen: 10 years, 2 months
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Re: How much spores per syringe? [Re: aww]
#20602405 - 09/22/14 05:45 PM (10 years, 3 months ago) |
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Is there a easy way to check if my syringe contains contamination?
Edited by PussyFart (09/22/14 05:49 PM)
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ant707
not a puppet


Registered: 07/26/14
Posts: 579
Loc: northeast florida
Last seen: 7 years, 11 months
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Re: How much spores per syringe? [Re: aww]
#20602425 - 09/22/14 05:48 PM (10 years, 3 months ago) |
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Quote:
PussyFart said: Heat up an inoculation loop to red hot, then cool it down in an agar plate, creating a small puddle in the center of the dish.
Then I scrape some spores with the loop, then touch the center of the agar plate with it, wiping in a circular motion, then seal up the dish with parafilm or 1" strips of saran wrap.
That is all.....then you just wait a week or so for growth and make some transfers.
All of this is done in a SAB(Still Air Box) of course.
cool. i recently got some one-time use cotton swabs to try swiping some spores on a plate. i need to invest in an inoculation loop
Quote:
aww said: Is there a easy way to check if my syringe contains contamination?
agar!
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: How much spores per syringe? [Re: aww]
#20602429 - 09/22/14 05:49 PM (10 years, 3 months ago) |
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Quote:
aww said: Is there a easy way to check if my syringe contains contamination?
Yes, inoculate something.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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Stropharis



Registered: 04/02/07
Posts: 1,854
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Re: How much spores per syringe? [Re: Thadeous]
#20602612 - 09/22/14 06:17 PM (10 years, 3 months ago) |
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Quote:
Thadeous said: See the scraped portion in this image? [taken from the link above] 
About that much. 
I was being generous, a little does indeed go a very long way. A fraction of that would suffice.
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PurePleasure
Untrusted bacteria cultivator.



Registered: 08/29/14
Posts: 5,893
Loc: Lost
Last seen: 1 year, 11 months
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Re: How much spores per syringe? [Re: PussyFart]
#20602866 - 09/22/14 07:00 PM (10 years, 3 months ago) |
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Quote:
PussyFart said: Heat up an inoculation loop to red hot, then cool it down in an agar plate, creating a small puddle in the center of the dish.
Then I scrape some spores with the loop, then touch the center of the agar plate with it, wiping in a circular motion, then seal up the dish with parafilm or 1" strips of saran wrap.
That is all.....then you just wait a week or so for growth and make some transfers.
All of this is done in a SAB(Still Air Box) of course.
-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

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PurePleasure
Untrusted bacteria cultivator.



Registered: 08/29/14
Posts: 5,893
Loc: Lost
Last seen: 1 year, 11 months
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Re: How much spores per syringe? [Re: PussyFart]
#20602874 - 09/22/14 07:01 PM (10 years, 3 months ago) |
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Quote:
PussyFart said:
Quote:
aww said: Is there a easy way to check if my syringe contains contamination?
Yes, inoculate something.
-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

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aww
Stranger


Registered: 07/04/14
Posts: 44
Last seen: 10 years, 2 months
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Re: How much spores per syringe? [Re: aww]
#20603051 - 09/22/14 07:30 PM (10 years, 3 months ago) |
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I will inoculate 6 glasses tomorrow Hopefully it will turn out fine.
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