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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 8 years, 1 month
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Would this work?
#20600455 - 09/22/14 10:07 AM (10 years, 3 months ago) |
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I tried to swab some pe spores from a spore swab to agar. Have no growth except a couple thta have some nasty looking bacteria growing.
Frustrated I put the swab in a agar dish and injected some sterile water in it..shook it up (hoping to free any spores) and drew up the water with my syringe. Do you think I may find some success with putting a couple drops on agar?
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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It'll likely be a pool of bacteria. I would sandwich it or use antibiotic agar.
Or......take another swab
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Re: Would this work? [Re: Ghatti]
#20600497 - 09/22/14 10:18 AM (10 years, 3 months ago) |
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Give it a few days to a week....I often get bacteria from swabs, but the spores usually still germinate.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 8 years, 1 month
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Re: Would this work? [Re: PussyFart]
#20600548 - 09/22/14 10:34 AM (10 years, 3 months ago) |
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Will do PF. Thanks.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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In my own recent experience with PE on agar, the spores definitely take a while to germinate.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 8 years, 1 month
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Re: Would this work? [Re: NumeroEno]
#20600662 - 09/22/14 11:05 AM (10 years, 3 months ago) |
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Eno thanks for your reply. I will let the jars sit a bit longer and hope for the best. About how many days? I hit some up on Thursday and did a few a more yesterday on Sunday.
Threw two jars down the sink that had the bacterial. I will be more patient going forward.
Edited by midnightmaraude (09/22/14 11:06 AM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Spores can take 2+ weeks in some cases to germinate.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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I inoculated 4 agar plates with PE on the 14th and am just now starting to see a little bit of growth.
--------------------
Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 8 years, 1 month
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Re: Would this work? [Re: NumeroEno]
#20600719 - 09/22/14 11:18 AM (10 years, 3 months ago) |
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oohhhhhh. ok
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