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Offlinepooskooper
newbie

Registered: 09/25/03
Posts: 50
Last seen: 11 years, 7 months
My syringe tech, see any problems?
    #2060055 - 10/31/03 10:18 AM (13 years, 1 month ago)

First I swab a piece of wax paper with h2o2, then with a sterile knife (h2o2 again), i cut the cap off, and place it on the wax paper, then place a clean jar over the cap. It sits overnight, then the cap is removed from the paper. The print is then allowed to dry for 12 hours. After I pc a syringe for 20 minutes, i fill it with h2o2, shake and empty, then fill it with spring water. the spring water is then injected into a clean jar, and the entire print is scraped into the jar with the needle from the syringe. Then after a few times of filling the syringe and empying it into the jar to break up the spores, I fill the syringe with the solution. Before it is used to inoc, it is flame sterilized until its red hot, then cooled with h202.

Where does the trich, get in?


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Somedays, you get lucky enough to deliver someone else's karma.


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Offlineangryjslice
now with 20%more anger
Male

Folding@home Statistics
Registered: 10/29/03
Posts: 916
Last seen: 8 months, 10 days
Re: My syringe tech, see any problems? [Re: pooskooper]
    #2060077 - 10/31/03 10:32 AM (13 years, 1 month ago)

you dont need to use the whole print. Use about 1/4 of the print. Its been talked about several times on this board that more spores does not neccisarily = better.

~JSlice~


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Offlinepooskooper
newbie

Registered: 09/25/03
Posts: 50
Last seen: 11 years, 7 months
Re: My syringe tech, see any problems? [Re: angryjslice]
    #2060084 - 10/31/03 10:37 AM (13 years, 1 month ago)

My theory is, that if something is wrong with the print, then Ive only screwed up one syringe, not all of them.


--------------------
Somedays, you get lucky enough to deliver someone else's karma.


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OfflinetwistedJay
hydriodic acid
Registered: 08/31/03
Posts: 153
Loc: IM A REAL KILLA
Last seen: 6 years, 10 months
Re: My syringe tech, see any problems? [Re: pooskooper]
    #2060279 - 10/31/03 12:35 PM (13 years, 1 month ago)

try sterilizing distilled water in a jar, then placing the mush cap on some wire just above the water in the jar, let sit until spores drop into water, take cap out and wire, put jar lid with one hole punched on with tape over hole.

when ever you want a syringe, just shake jar and suck some out.

this way you can get many syringes easier.


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this has been a message from a twisted ass mutha fucka. and if you dont like it.........EAT A DICK!


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Offlinewackytron
journeyman

Registered: 09/05/03
Posts: 92
Last seen: 11 years, 8 months
Re: My syringe tech, see any problems? [Re: twistedJay]
    #2061080 - 10/31/03 05:37 PM (13 years, 1 month ago)

wow, anyone have any comments on the tek above ^^^^. I like this idea!


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Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: My syringe tech, see any problems? [Re: wackytron]
    #2061258 - 10/31/03 06:51 PM (13 years, 1 month ago)

pooskooper -- spring water isn't sterile -- the only problem I see. I'd sterilize it first....

Jay -- sounds good! Only comment I have is to be sterile, and don't take prints from a casing.

--
Micro


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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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OfflineCallampin
Psyco Myco
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Registered: 08/14/03
Posts: 520
Last seen: 7 months, 11 days
Re: My syringe tech, see any problems? [Re: micro]
    #2061393 - 10/31/03 08:07 PM (13 years, 1 month ago)

yea the syringe making thing is a fukin good idea its a fukin awesome tek that lil kushh made som etime ago
lil kush's syringe tek


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Invisibleniteowl
GrandPaw
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Registered: 07/01/03
Posts: 16,291
Loc: Flag
Re: My syringe tech, see any problems? [Re: Callampin]
    #2061488 - 10/31/03 08:45 PM (13 years, 1 month ago)

Quote:

Callampin said:
yea the syringe making thing is a fukin good idea its a fukin awesome tek that lil kushh made som etime ago
lil kush's syringe tek





I read that thread and am taking prints using that TEK. I will make the syringe tonite after the trick or treaters stop.


--------------------
Live for the moment you are in now
Don't be bogged down by your past
Don't be afraid of what lies in your future


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OfflineCallampin
Psyco Myco
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Registered: 08/14/03
Posts: 520
Last seen: 7 months, 11 days
Re: My syringe tech, see any problems? [Re: niteowl]
    #2062380 - 11/01/03 09:13 AM (13 years, 1 month ago)

ive done it twice already i works wonderfully , nice dark syringes :laugh:


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Invisibleniteowl
GrandPaw
Male User Gallery

Registered: 07/01/03
Posts: 16,291
Loc: Flag
Re: My syringe tech, see any problems? [Re: Callampin]
    #2062563 - 11/01/03 01:21 PM (13 years, 1 month ago)

The shroom fell off the nail and went into the water! Im not so sure that I want to use it now.


--------------------
Live for the moment you are in now
Don't be bogged down by your past
Don't be afraid of what lies in your future


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