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Offlinemidnightmaraude
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Would this work?
    #20600455 - 09/22/14 10:07 AM (10 years, 3 months ago)

I tried to swab some pe spores from a spore swab to agar.  Have no growth except a couple thta have some nasty looking bacteria growing.

Frustrated I put the swab in a agar dish and injected some sterile water in it..shook it up (hoping to free any spores) and drew up the water with my syringe.  Do you think I may find some success with putting a couple drops on agar?

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InvisibleGhatti
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Re: Would this work? [Re: midnightmaraude]
    #20600462 - 09/22/14 10:09 AM (10 years, 3 months ago)

It'll likely be a pool of bacteria. I would sandwich it or use antibiotic agar.

Or......take another swab

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OfflinePussyFart
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Re: Would this work? [Re: Ghatti]
    #20600497 - 09/22/14 10:18 AM (10 years, 3 months ago)

Give it a few days to a week....I often get bacteria from swabs, but the spores usually still germinate.

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Offlinemidnightmaraude
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Re: Would this work? [Re: PussyFart]
    #20600548 - 09/22/14 10:34 AM (10 years, 3 months ago)

Will do PF. Thanks.

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InvisibleNumeroEno
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Re: Would this work? [Re: midnightmaraude]
    #20600595 - 09/22/14 10:46 AM (10 years, 3 months ago)

In my own recent experience with PE on agar, the spores definitely take a while to germinate.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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Offlinemidnightmaraude
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Re: Would this work? [Re: NumeroEno]
    #20600662 - 09/22/14 11:05 AM (10 years, 3 months ago)

Eno thanks for your reply.  I will let the jars sit a bit longer and hope for the best.  About how many days?  I hit some up on Thursday and did a few a more yesterday on Sunday.

Threw two jars down the sink that had the bacterial.  I will be more patient going forward. 

Edited by midnightmaraude (09/22/14 11:06 AM)

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OfflinePussyFart
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Re: Would this work? [Re: midnightmaraude]
    #20600686 - 09/22/14 11:09 AM (10 years, 3 months ago)

Spores can take 2+ weeks in some cases to germinate.

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InvisibleNumeroEno
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Re: Would this work? [Re: midnightmaraude]
    #20600694 - 09/22/14 11:12 AM (10 years, 3 months ago)

I inoculated 4 agar plates with PE on the 14th and am just now starting to see a little bit of growth.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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Offlinemidnightmaraude
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Re: Would this work? [Re: NumeroEno]
    #20600719 - 09/22/14 11:18 AM (10 years, 3 months ago)

oohhhhhh. ok

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