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InvisibleBsdgaou

Registered: 02/20/19
Posts: 304
Re: Reishi find, clone, and grow [Re: TheGrower]
    #26966747 - 10/03/20 12:18 AM (1 month, 30 days ago)

Quote:

TheGrower said:
Ok something is happening, does this look ok?
I used 5g LME and 5g agar : 250ml water.
About 6 days in.

Thanks






Take another transfer from 9 or 11 o'clock.


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OfflineQuadman
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Re: Reishi find, clone, and grow [Re: Bsdgaou]
    #26966782 - 10/03/20 12:52 AM (1 month, 30 days ago)

:Looks like mycelium,  just really variable. To get a nice culture I would take a transfer from about 11 o'clock,  you should be in great shape then imo.:lol::whathesaid:


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Edited by Quadman (10/03/20 12:54 AM)


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OfflineTheGrower
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Registered: 04/08/20
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Re: Reishi find, clone, and grow [Re: Quadman]
    #26986428 - 10/15/20 05:25 AM (1 month, 18 days ago)

I took the transfers.
One is going ok the other one contaminated on the outer edge. Funny enough the first one I took transfers from fully colonised it looks great.
I’ll post pics later.
Thanks all.


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OfflineTheGrower
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Registered: 04/08/20
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Re: Reishi find, clone, and grow [Re: TheGrower]
    #27022856 - 11/05/20 10:13 AM (28 days, 4 hours ago)

As above. I forgot to take photos of the first two that I cut up and added to ssd blocks. This is the third one I took that I’m going to use to make more transfers and inoculate a log outside.
Updates on the blocks as and when 👍🏻
Thanks



Ps it was so hard to open the Petri dish lol like the mycelium had taken a hold of the lid is that normal?


Edited by TheGrower (11/05/20 10:44 AM)


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OfflineForrester
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Re: Reishi find, clone, and grow [Re: TheGrower]
    #27026077 - 11/07/20 05:05 AM (26 days, 10 hours ago)

Yeah that happens quite a bit


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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Invisiblettching8475
Spawn
Registered: 03/01/18
Posts: 691
Re: Reishi find, clone, and grow [Re: Forrester]
    #27026172 - 11/07/20 06:57 AM (26 days, 8 hours ago)

I found a tree near my house growing conks. For a cloning attempt, use the lighter, leading edge growth on the outside of the conk rather than inner tissue?


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OfflineForrester
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Re: Reishi find, clone, and grow [Re: ttching8475]
    #27027067 - 11/07/20 04:33 PM (25 days, 22 hours ago)

Quote:

ttching8475 said:
For a cloning attempt, use the lighter, leading edge growth on the outside of the conk rather than inner tissue?




No, you want the white growth edge but the inside of it, not the outside of the mushroom that's touched air and all the crap in it.


--------------------
Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Invisiblettching8475
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Registered: 03/01/18
Posts: 691
Re: Reishi find, clone, and grow [Re: Forrester]
    #27028045 - 11/08/20 08:17 AM (25 days, 6 hours ago)

Thanks. I phrased that poorly. Always clone with inner tissues :facepalm:


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OfflineTheGrower
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Re: Reishi find, clone, and grow [Re: ttching8475]
    #27050584 - 11/21/20 04:00 PM (11 days, 23 hours ago)

Hello.
One of my blocks contaminated the other is still colonising slowly but surely.
The last plate I used in a quart jar with some brf and vermiculite that I wanted to use up.
It absolutely tore through the brf and verm! Not sure if anyone’s used brf and verm with reishi but it does perform well at least this one has.
Now I’m about to do a little g2g into rye jars that I made up last night. And with the rest of this fully colonised cake I will spawn to a bucket of oak wood chips enriched with wheat bran and some gypsum. Smells so beautiful!
Here’s the quart pf cake.
Thanks



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OfflineForrester
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Re: Reishi find, clone, and grow [Re: TheGrower]
    #27052333 - 11/22/20 03:09 PM (10 days, 23 hours ago)

Careful spawning to a substrate with wheat bran in it, unless it's sterilized and you're doing so in front of a flow hood, which since you said it's a bucket I assume isn't the case. 

Reishi really isn't good at fighting off molds and spawning to a supplemented substrate without sterilizing it is asking for mold.  I would leave the wheat bran out.


--------------------
Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineTheGrower
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Registered: 04/08/20
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Re: Reishi find, clone, and grow [Re: Forrester]
    #27052461 - 11/22/20 04:46 PM (10 days, 22 hours ago)

Damn forrester your too late. I really appreciate the advice but I already did the spawn and g2g.
I pasteurised the sawdust/bran/gypsum doing the bucket tek. So I can just hope it does not contaminate.
If it does I will leave the bran out next time. I still have two rye quart jars and three agar plates that are colonising quite nicely so all is not lost.

Cheers mate.


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OfflineTheGrower
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Re: Reishi find, clone, and grow [Re: TheGrower]
    #27054084 - 11/23/20 03:38 PM (9 days, 23 hours ago)

Also check this cool colouring on one of my plates. Is it normal?
Thanks


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OfflineForrester
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Re: Reishi find, clone, and grow [Re: TheGrower]
    #27054299 - 11/23/20 06:20 PM (9 days, 20 hours ago)

Not sure, might be some bacteria in there but that's not a worry if it's reishi.


--------------------
Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineMrcloudy
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Re: Reishi find, clone, and grow [Re: TheGrower]
    #27057861 - 11/25/20 10:51 PM (7 days, 16 hours ago)

Quote:

TheGrower said:
Also check this cool colouring on one of my plates. Is it normal?
Thanks






Perfectly normal, and quite common in certain strains and species.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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