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Offlineghiajake
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Re: Reishi find, clone, and grow [Re: ghiajake]
    #20607751 - 09/23/14 08:12 PM (7 years, 7 months ago)

See if they'll let you mulch around the tree and under the grate.


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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20607852 - 09/23/14 08:27 PM (7 years, 7 months ago)



Some more trees that had the fungus on them. the first few pics are of the same tree but there was about 4 other trees with the ganoderma on them.


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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20607861 - 09/23/14 08:29 PM (7 years, 7 months ago)

sorry about the blue tinge had the white balance all fucked up didn't realize til way later


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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20607957 - 09/23/14 08:49 PM (7 years, 7 months ago)

Fuck I had an anne halonium flashback :crazy2:


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InvisiblebodhisattaMDiscordReddit
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Re: Reishi find, clone, and grow [Re: Pastywhyte]
    #20607975 - 09/23/14 08:54 PM (7 years, 7 months ago)

haha,

So far none of the clone tissue dishes I started on the 20th have grown out yet. one of the 4 dishes got some bacteria the other ones the clone tissue is just still sitting there. I may have to do more. Any suggestions for getting this clone tissue to get moving on agar. Or a different agar recipe. My lucidum cultures eat a dish in 3 days max.


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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20607986 - 09/23/14 08:56 PM (7 years, 7 months ago)

I would just play with some different media. Try dog food agar. All my cultures are tearing threw it right now.


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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20607991 - 09/23/14 08:56 PM (7 years, 7 months ago)

Wilds can be tricky. I have had some really take their sweet ass time to recover. Some never do. I like tetra agar for wild cloning.


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Re: Reishi find, clone, and grow [Re: Pastywhyte]
    #20608020 - 09/23/14 09:04 PM (7 years, 7 months ago)

Yea I bet I can find it at the pet store. I would do DFA but I don't have a dog haha. I do have cat food. I tried cat food agar but had really no luck with it.


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Offlineghiajake
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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20608182 - 09/23/14 09:38 PM (7 years, 7 months ago)

I was told by Tradd Cotter in Telluride that tissue from wild conks doesn't like to recover. The best way to collect tissue is active mycelium, so try to get some of the bark or wood with myc in it. This is why I suggest to add mulch around known fruiting sites. The myc will colonize the mulch and you can use that to culture from. You can also soak a toothpick and stick it to the leading white edge of the conk you want to sample. Once the conk has grown around the toothpick twist and pull it free and use that to clone from.


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Re: Reishi find, clone, and grow [Re: ghiajake]
    #20608244 - 09/23/14 09:55 PM (7 years, 7 months ago)

Quote:

ghiajake said:
I was told by Tradd Cotter in Telluride that tissue from wild conks doesn't like to recover. The best way to collect tissue is active mycelium, so try to get some of the bark or wood with myc in it. This is why I suggest to add mulch around known fruiting sites. The myc will colonize the mulch and you can use that to culture from. You can also soak a toothpick and stick it to the leading white edge of the conk you want to sample. Once the conk has grown around the toothpick twist and pull it free and use that to clone from.



:thumbup:

awesome. If the tree is fucked anyway I'll just cut out a chunk of root and look for mycelium. I suppose I'll really need antibiotic agar for that,


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Offlineghiajake
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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20608279 - 09/23/14 10:04 PM (7 years, 7 months ago)

Instead of taking it from the tree to agar, try doing this. Then once it's colonized you have clean culture to sample.


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Re: Reishi find, clone, and grow [Re: ghiajake]
    #20608286 - 09/23/14 10:07 PM (7 years, 7 months ago)

Quote:

ghiajake said:
Instead of taking it from the tree to agar, try doing this. Then once it's colonized you have clean culture to sample.



:bow:


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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20608297 - 09/23/14 10:11 PM (7 years, 7 months ago)

:takingnotes:


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Re: Reishi find, clone, and grow [Re: azur]
    #20608325 - 09/23/14 10:16 PM (7 years, 7 months ago)

I know where I'm going after work tomorrow

well first I need paper bags


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Edited by Trusted cuItivator (09/23/14 10:17 PM)


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OfflineMrcloudy
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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20608844 - 09/24/14 12:06 AM (7 years, 7 months ago)

I have had luck with tissues from conk so long as the tissue is viable. It can be very hard especially with other Ganoderma sp. to determine if the tissue is still going to be viable. First 4 conks I ever cloned from grew just fine with no problems even though they were fully mature, and sat in my fridge for a week or two before I could get to them.

Time, It can take some times for the tissue to remycelialize ( I don't think that's a word) Once it does it will explode in growth. How have your toothpick clones been working for you Jake?


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10 different Ganoderma species from across the USA

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MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflineMrcloudy
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
    #20608861 - 09/24/14 12:09 AM (7 years, 7 months ago)

Sticking it in the white growth edge actually seems like a pretty good idea if I will be around the conk again. Many times I either find a conk and need to take it home to clone it due to being out of town, or its white edge has been gone for a bit.I might start telling people to do that when they leave the mushroom in situ for some of those interesting collections that always arrive unviable.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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Offlineghiajake
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
    #20609007 - 09/24/14 12:34 AM (7 years, 7 months ago)

I haven't tried it yet, but that was another tip from Tradd Cotter so I trust it. Makes sense enough to be valid.


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Re: Reishi find, clone, and grow [Re: ghiajake]
    #20668150 - 10/06/14 09:25 PM (7 years, 7 months ago)

been having to neglect my culture for a while too busy with other things but I'll be getting to work with it in a few days, It finally "re-mycelialized"



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InvisibleJosh.0
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Re: Reishi find, clone, and grow [Re: bodhisatta]
    #20668549 - 10/06/14 10:49 PM (7 years, 7 months ago)

those plates look great Bodhisatta! :awetongue:
could you specify the whereabouts you took your tissue from:

did you transfer any myc from the stembutt or was it strictly inner tissue? and what is your agar of choice for this?
I ask because im about to try my hand at this in mere moments following your response!
:sunspots:


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Offlineblojo02184
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Re: Reishi find, clone, and grow [Re: Josh.0]
    #20670231 - 10/07/14 09:16 AM (7 years, 7 months ago)

nice dude
my wild find took almost 10 days to revert back to colonization stage...

plus I had bacteria and competing molds.

the reishi ate it all... making it a hitch to transfer clean pieces lol


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