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 Stropharis
Registered: 04/02/07
Posts: 1,854
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 Re: Reishi find, clone, and grow  [Re: KindofBlue]
#21577500 - 04/21/15 06:13 PM (8 years, 11 months ago) |
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Quote:
KindofBlue said:
this thread is awesome...seriously. To me this is what it's all about.
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bodhisatta  
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Reishi find, clone, and grow [Re: Stropharis]
#21577539 - 04/21/15 06:26 PM (8 years, 11 months ago) |
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Quote:
Stropharis said:
Quote:
KindofBlue said:
this thread is awesome...seriously. To me this is what it's all about.
I'll upload some microscopy of that plate with the spores germination. I made 5 plates and they're all clean so I figures I could butcher one for science
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 Mrcloudy
Stranger than you.
Registered: 10/01/13
Posts: 2,889
Loc: Northeast US 
Last seen: 6 months, 4 days
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Re: Reishi find, clone, and grow [Re: bodhisatta]
#21577559 - 04/21/15 06:31 PM (8 years, 11 months ago) |
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I tried a multispore wood shaving tub. I basically just added pasterized wood shavings to my previous tub that was loaded with spores, I got zero germination, so it is definitely a good idea to start them on agar first. It will be easiest with the G. resinaceum group. Other species, G.tsugae in particular are near impossible to germinate.
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10 different Ganoderma species from across the USA
AMU
MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
#21577567 - 04/21/15 06:33 PM (8 years, 11 months ago) |
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Quote:
Mrcloudy said:
I tried a multispore wood shaving tub. I basically just added pasterized wood shavings to my previous tub that was loaded with spores, I got zero germination, so it is definitely a good idea to start them on agar first. It will be easiest with the G. resinaceum group. Other species, G.tsugae in particular are near impossible to germinate.
What is really strange is calcium oxalate(my assumption) crystals are everywhere when I took a look at the mycelium. You'll see in a few hours when I upload but its like the mycelium sequesters Ca and did it itself
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Reishi find, clone, and grow [Re: bodhisatta]
#21577655 - 04/21/15 06:51 PM (8 years, 11 months ago) |
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so the pictures are kind of shitty. I was in a rush. I did use 10% KOH solution to try to make some nicer looking pictures but  I didn't really take my time with it.
I couldn't find any spore germinating or spore with hyphae coming out of it. I got close but I'll have to try again later to get a real good picture of a spore in germination.
I couldn't find any clamp connections either. I know not every species of mushroom makes them but I may have just not seen any either. Does any one else know if the ganoderma species make clamp connections?
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Mrcloudy
Stranger than you.
Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 6 months, 4 days
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Re: Reishi find, clone, and grow [Re: bodhisatta]
#21577816 - 04/21/15 07:23 PM (8 years, 11 months ago) |
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Yes Ganoderma make clamp connections, they can be hard to observe at times though. Calcium oxalate, would that be diamond shaped crystals? I get those frequently but never really thought about what they might be.
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10 different Ganoderma species from across the USA
AMU
MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
#21580470 - 04/22/15 02:38 PM (8 years, 11 months ago) |
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Quote:
Mrcloudy said:
Yes Ganoderma make clamp connections, they can be hard to observe at times though. Calcium oxalate, would that be diamond shaped crystals? I get those frequently but never really thought about what they might be.
yea if you look up Ca Oxilate pics on google. but I see oxilate all the time in other things and it looks exactly the same, but i have never seen it on agar plate scrapings, or ever on YPD for that matter which leads me to believe it's the resinaceium itself causing that?
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Mrcloudy
Stranger than you.
Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 6 months, 4 days
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Re: Reishi find, clone, and grow [Re: bodhisatta]
#21580584 - 04/22/15 03:12 PM (8 years, 11 months ago) |
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Could be, to be honest I haven't paid much attention, but I do see them when I am looking for chlamydospores, which I typically only look for if I suspect it is in the resinaceum group, maybe I will start paying more attention. Thanks for solving the mystery of what those are! 
All my strains are a little too grown out for microscopy at the moment. I think I will do a comparative growth set to see if they form with other species, I kind of just assumed they were something in the agar precipitating. But haven't been looking at agar plates under the microscope enough.
Very cool.
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10 different Ganoderma species from across the USA
AMU
MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
#21580589 - 04/22/15 03:13 PM (8 years, 11 months ago) |
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yea if you could eventually get some data in too that would be cool. It might just be some artifact I somehow got in there.
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Mrcloudy
Stranger than you.
Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 6 months, 4 days
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Re: Reishi find, clone, and grow [Re: bodhisatta]
#21580661 - 04/22/15 03:29 PM (8 years, 11 months ago) |
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What kind of agar did you use? Mine is typically MEA
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10 different Ganoderma species from across the USA
AMU
MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
#21580698 - 04/22/15 03:38 PM (8 years, 11 months ago) |
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YPD.
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Mrcloudy
Stranger than you.
Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 6 months, 4 days
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Re: Reishi find, clone, and grow [Re: bodhisatta]
#21580716 - 04/22/15 03:43 PM (8 years, 11 months ago) |
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So the only thing in common is the mushroom. I just checked an old MEA plate that nothing ever grew on, no crystals to be found, if it was something in the agar it has had plenty of time to precipitate. I think it is caused by the mushroom.
--------------------
10 different Ganoderma species from across the USA
AMU
MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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Mrcloudy
Stranger than you.
Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 6 months, 4 days
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
#21580720 - 04/22/15 03:44 PM (8 years, 11 months ago) |
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D you think Calcium oxalate might be present in the fruit as well?
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10 different Ganoderma species from across the USA
AMU
MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
#21580738 - 04/22/15 03:48 PM (8 years, 11 months ago) |
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perhaps. It's very insoluble in water so coming from the agar itself seems really strange. and I've never seen it on anything I've taken from a YPD plate before.
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Mrcloudy
Stranger than you.
Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 6 months, 4 days
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Re: Reishi find, clone, and grow [Re: bodhisatta]
#21580774 - 04/22/15 03:58 PM (8 years, 11 months ago) |
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Log in to view attachment
I found this study when I looked up Calcium oxalate and Ganoderma, it would appear there are several wood rotting mushrooms that produce it on agar.
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10 different Ganoderma species from across the USA
AMU
MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
#21580845 - 04/22/15 04:16 PM (8 years, 11 months ago) |
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interesting.
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knomadic_niki
A mile high
Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 8 months
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Re: Reishi find, clone, and grow [Re: Mrcloudy]
#21580851 - 04/22/15 04:17 PM (8 years, 11 months ago) |
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very interesting, guys! that's it! i'm buying a scope!
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My trade list
In search of sporeless oyster cultures
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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http://www.amazon.com/Omano-OM157-T-Trinocular-Semi-Plan-Microscope/dp/B006I6YDEY
I want a phase contrast scope but I don't think I can save up for one, this one had my eye though
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knomadic_niki
A mile high
Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 8 months
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Re: Reishi find, clone, and grow [Re: bodhisatta]
#21580974 - 04/22/15 04:48 PM (8 years, 11 months ago) |
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what makes that one stand out, bod? i'm not sure what to get. i've got about a $500 budget for it
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My trade list
In search of sporeless oyster cultures
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Mrcloudy
Stranger than you.
Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 6 months, 4 days
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http://www.ebay.com/itm/AmScope-40X-2500X-LED-Lab-Binocular-Compound-Microscope-w-3D-2-Layer-Mech-Stage-/400757059900?pt=LH_DefaultDomain_0&hash=item5d4efb713c
Here is what I got, price has gone down a bit since I got it. 
If you have a $500 budget though you could probably get a higher quality one. The one Bodhi posted is probably good especially if you have a camera for it.
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10 different Ganoderma species from across the USA
AMU
MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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