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SpinScratch
Distrusted Cultivator


Registered: 07/26/13
Posts: 834
Last seen: 7 months, 17 days
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Spore Syringe to grain?
#20593282 - 09/20/14 03:45 PM (10 years, 3 months ago) |
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Ive inoculated probably 20 quart grain jars with 1-2cc with no shake from syringes of varying sources. Id say i have about a 25% germination rate of my jars after weeks of waiting. The subsequent g2g transfers are going fine, which leads me to believe its not a matter of WBS prep or sterile technique. So my point is... Is this the rate of germination to be expected when using spores to grain?
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 3 hours, 6 minutes
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Re: Spore Syringe to grain? [Re: SpinScratch]
#20593293 - 09/20/14 03:47 PM (10 years, 3 months ago) |
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as in 25% of your jars have growth?
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Dekozn
Stranger than kindness


Registered: 04/12/06
Posts: 1,183
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Re: Spore Syringe to grain? [Re: SpinScratch]
#20593320 - 09/20/14 03:52 PM (10 years, 3 months ago) |
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I never had a jar that didn't germinate, had lots of contams though due to my technique. Always used MS inoculation so maybe it's your vendor or you don't shake the syringes but even when I forgot to shake I had germination. Maybe your jars weren't cooled down or the way u store your spores....
-------------------- Organized people are just to lazy to search for their stuff...
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SpinScratch
Distrusted Cultivator


Registered: 07/26/13
Posts: 834
Last seen: 7 months, 17 days
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Re: Spore Syringe to grain? [Re: cronicr]
#20596401 - 09/21/14 09:55 AM (10 years, 3 months ago) |
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Quote:
cronicr said: as in 25% of your jars have growth?
Yes. But G2G is 100%
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SpinScratch
Distrusted Cultivator


Registered: 07/26/13
Posts: 834
Last seen: 7 months, 17 days
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Re: Spore Syringe to grain? [Re: Dekozn]
#20596408 - 09/21/14 09:58 AM (10 years, 3 months ago) |
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Quote:
Dekozn said: I never had a jar that didn't germinate, had lots of contams though due to my technique. Always used MS inoculation so maybe it's your vendor or you don't shake the syringes but even when I forgot to shake I had germination. Maybe your jars weren't cooled down or the way u store your spores....
8 different syringes from 2 different vendors, well shaken, Jars cooled overnight, syringes stored in fridge.
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bloodyice69
GrassHopper



Registered: 08/06/14
Posts: 296
Loc: Colonizing Jars
Last seen: 4 years, 2 months
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Re: Spore Syringe to grain? [Re: SpinScratch]
#20596463 - 09/21/14 10:10 AM (10 years, 3 months ago) |
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Quote:
SpinScratch said: Ive inoculated probably 20 quart grain jars with 1-2cc with no shake from syringes of varying sources. Id say i have about a 25% germination rate of my jars after weeks of waiting. The subsequent g2g transfers are going fine, which leads me to believe its not a matter of WBS prep or sterile technique. So my point is... Is this the rate of germination to be expected when using spores to grain?
im not sure BUT. i innoc'd 2 3lb 5-grain spawn bags, lost one to contam. but the other one took well into 50ish days before it was colonized.
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Dekozn
Stranger than kindness


Registered: 04/12/06
Posts: 1,183
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Re: Spore Syringe to grain? [Re: bloodyice69]
#20596863 - 09/21/14 12:14 PM (10 years, 3 months ago) |
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Yeah how long ago did you knock them up?
-------------------- Organized people are just to lazy to search for their stuff...
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bloodyice69
GrassHopper



Registered: 08/06/14
Posts: 296
Loc: Colonizing Jars
Last seen: 4 years, 2 months
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Re: Spore Syringe to grain? [Re: Dekozn]
#20596873 - 09/21/14 12:15 PM (10 years, 3 months ago) |
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7/22 .
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 3 hours, 6 minutes
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Re: Spore Syringe to grain? [Re: cronicr]
#20596901 - 09/21/14 12:22 PM (10 years, 3 months ago) |
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Quote:
cronicr said: as in 25% of your jars have growth?
multispore in a nutshell, i'd just g2g the fuck out of what worked
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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