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jordano
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Registered: 08/06/14
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Last seen: 10 years, 1 month
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Agar recipe, lower nute, back to normal?
#20587757 - 09/19/14 10:29 AM (10 years, 3 months ago) |
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Hey guys, I want to say thanks in advance to anyone who answers me! So I started some agar work recently, I have some dishes done, one transfer has been completed already. My first agar was a regular MEA recipe (30G ME, 20G agar, 1000ml water.) transferred into a lower nute recipe (20G ME, 20G agar, 1000ml water.) it worked great i have much better rhizomorphic growth in my new petris and only about 4 sectors in the dish im choosing for further isolation. So now that you guys have a run through of the situation my questions are.
1) Now that I have good rhizo growth in my dish, should I bring the nutrients in the agar back up to where it used to be? (30G ME)
2) I'm adding rye flour to the new agar I'm making, would the extra nutrients change anything as far as growth goes? (took handful of flour, let it sit in boiling water for 10 mins/strained and using 50ml of rye water in recipe.)
Edited by jordano (09/19/14 11:17 AM)
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 10 hours, 33 minutes
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Re: Agar recipe, lower nute, back to normal? [Re: jordano]
#20587772 - 09/19/14 10:33 AM (10 years, 3 months ago) |
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damn i use 20 grams mea 500 ml water you use 40 grams per 500 ml water
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nksfo5
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Registered: 12/19/12
Posts: 689
Last seen: 10 months, 23 days
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Re: Agar recipe, lower nute, back to normal? [Re: tripdawg420]
#20587808 - 09/19/14 10:44 AM (10 years, 3 months ago) |
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Damn I only use 20grams mea and 20grams agar for 1000ml water
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nksfo5
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Registered: 12/19/12
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Last seen: 10 months, 23 days
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Re: Agar recipe, lower nute, back to normal? [Re: nksfo5]
#20587814 - 09/19/14 10:46 AM (10 years, 3 months ago) |
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If your getting good growth leave the nutrient alone As far as the rye water goes. Just why? Experiment?
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 10 hours, 33 minutes
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Re: Agar recipe, lower nute, back to normal? [Re: nksfo5]
#20587818 - 09/19/14 10:47 AM (10 years, 3 months ago) |
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thats alot per 500 ml water if you wana get less nutes try like 18 grams of mea per 500 ml but 20 is real nice
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jordano
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Registered: 08/06/14
Posts: 55
Last seen: 10 years, 1 month
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Re: Agar recipe, lower nute, back to normal? [Re: tripdawg420]
#20587888 - 09/19/14 11:05 AM (10 years, 3 months ago) |
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DOWNS44 said: Malt Extract Agar (MEA) 10 grams light malt extract 9 grams agar agar 500 ml potable or distilled water
MEPHISTOPHELIAN said: 30g ME 15g Agar 1000 ML water
FASTFREAD said: MEA (FDA M93) IS...
30 g Malt extract 20 g Agar 1 L Distilled water
Anything else is modified MEA. down44's would be 2% MEA and Mephistophelian's would be soft MEA.
MEA is commonly used in ranges from 1-4%.
I added in the Rye flour because I read that when myc eats it develops enzymes to break things down. (or something along the lines of that.) So basically your mycelium will be used to breaking down the rye grain, so it should break down the rye faster or start colonizing faster because it's not such a shock to run into a new material and have to "figure out" how to digest it.
Maybe you're right and I should just stick to the same mix of nutrients. Have you ever tried changing your agar ingredients? or do you have much experience with agar?
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jordano
Stranger


Registered: 08/06/14
Posts: 55
Last seen: 10 years, 1 month
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Re: Agar recipe, lower nute, back to normal? [Re: jordano]
#20587895 - 09/19/14 11:07 AM (10 years, 3 months ago) |
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lol i wrote 500ml of water and i actually did 1l :P my bad didn't catch that!
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nksfo5
traverse traveler


Registered: 12/19/12
Posts: 689
Last seen: 10 months, 23 days
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Re: Agar recipe, lower nute, back to normal? [Re: jordano]
#20594211 - 09/20/14 07:11 PM (10 years, 3 months ago) |
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If your getting good results I would just leave the majority as is and only experiment with a few plates rather than a whole batch so that you still have something viable to work with as well as something to experiment wirh
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