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Bloomer
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Bruised myc or contamination? Grain jar
    #20586546 - 09/18/14 11:55 PM (10 years, 3 months ago)

I had a few jars that were taking longer than others so I gave them a late, partial shake to redistribute the stuck grains. This is a week later and I'm ready to spawn and this jar looks all good except for one crevice was darker and colored. it was in an area where a large, unbroken myc ball had settled after the shake, leaving a little gap between the grains below. I'm preparing them to spawn, so I carefully shook this one apart a bit to get a better look at the inside of the crevice. This is what it looks like:


This jar in particular had a big ball that I left unbroken, it looks like it could just be blue bruising from the previous shake? Or a contam? I smelled the jar but I'm about 10 jars in so my tolerance for the smell is pretty high already and it's hard to tell if I can smell anything at all. I got a light whiff of mushrooms, but that's not surprising since it had a ton of healthy looking myc.

Thanks!

Edited by Icon (09/19/14 01:33 AM)

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Re: Bruised myc or contamination? Grain jar [Re: Icon]
    #20586597 - 09/19/14 12:26 AM (10 years, 3 months ago)

Another jar w/ similar, suspicious looking spot...


I have a different jar from this batch that I separated last week because it had a spot of trich, but it looks different than these spots, so I'm not sure what this could be. Anyone have any ideas?

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Re: Bruised myc or contamination? Grain jar [Re: Icon]
    #20586755 - 09/19/14 01:58 AM (10 years, 3 months ago)

i think that is a sign of bacteria. I had it happen in some of my jars as well and i just tossed them as it's not worth it to me to see the outcome. If you insist on using the suspicious jars isolate them (fruit separately) and you may get some fruits from them still. JUST DON"T G2G as you'll just be wasting more material and will make the infection worse and spread it farther. Go to agar if you haven't yet as grains are much easier/reliable when you have a good clean culture and of course good sterile tech (SAB)


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Edited by tombosley8 (09/19/14 02:01 AM)

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Re: Bruised myc or contamination? Grain jar [Re: tombosley8]
    #20586795 - 09/19/14 02:36 AM (10 years, 3 months ago)

What kind of bacteria? These jars are products of agar plates, g2g'd in a SAB.
Can you tell me more about the signs of this bacteria or where I can learn more about it? I'm going to leave these separate and spawn the rest. Thankfully it's only these 2 jars that have the spots out of 36, so that's pretty good but it's so subtle... how many other jars may sneak under the radar? My last round of tubs contaminated to something invisible and foul, probably bacteria; so I'm trying my best learn about and detect any problems. Thanks for your help.

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Re: Bruised myc or contamination? Grain jar [Re: Icon]
    #20586817 - 09/19/14 03:04 AM (10 years, 3 months ago)

Quote:

Icon said:





put some socks on.
those look sketch to me andthat last picture center right looks like mold growth


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
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Re: Bruised myc or contamination? Grain jar [Re: cronicr]
    #20586888 - 09/19/14 04:26 AM (10 years, 3 months ago)

Alright, thanks for the second opinions guys. Glad I asked and separated them. I'll probably still fruit them alone to so I know what would happen. Seeing these little spots gets me very nervous though because my last grow had similar problems. Most contaminated jars showed themselves very late after shaking. And even after careful checking and separation the survivors must have still been harboring hidden contams because 2/3 of the tubs turned bad after a couple weeks. These spots aren't as bad looking as the contams from my last round, but still the hidden factor drives me crazy whether innocent looking jars are about to ruin these upcoming tubs as well.

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Re: Bruised myc or contamination? Grain jar [Re: Icon]
    #20586937 - 09/19/14 05:09 AM (10 years, 3 months ago)

Why are they upside down on tyvek lids? I don't trust tyvek.

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Re: Bruised myc or contamination? Grain jar [Re: Sockadin]
    #20586950 - 09/19/14 05:21 AM (10 years, 3 months ago)

Quote:

Sockadin said:
Why are they upside down on tyvek lids? I don't trust tyvek.



It's just the angle I'm holding the jar so I could get a decent picture. I decided to try tyvek this time because of the bad contam rate on my last round of jars with polyfill. The tyvek appear to be doing better, but I did more research and that seems to be a common opinion that they're not trustworthy. I've got SFD's for the next round, that sounds like the best.

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Re: Bruised myc or contamination? Grain jar [Re: Icon]
    #20586958 - 09/19/14 05:25 AM (10 years, 3 months ago)

i feel you as i've been struggling recently with same type of issues. No signs in the jars but once in bulk especially once fruited everything went bad. I started fresh from new spores and it seems like i was able to get clean myc that i will have tested very soon. I think your best bet is to really get down on the agar and transfers and close inspections of the dishes and have multiple cultures from multiple sources (different spores, clones) in case one may have the hidden contam. IDK but I think it's pretty hard to contam another culture with a hiding contam (no spores) if you flame sterilize your scalpel. Maybe mold mycelium and spores are invisible (Hiding) and able to float and blow into another dish in some cases so i may be completely off but i think mold spores are usually pretty apparent. But mold just may hide until the last stage and then rampage and sporelate all over the place. The mold mycelium is what is hard to catch on agar sometimes because they are both white and it "rides along" behind the scenes.  Of course it could be your inoculation process and for whatever reason spores are getting in. You should be able to track down the culprit if you try spreading out your culture work to multiple sources and make sure everything is up to par on your sterile game. I'm hopin after nearly months i've come across another clean culture.
SFD's for the wiiiiin


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Edited by tombosley8 (09/19/14 05:27 AM)

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Re: Bruised myc or contamination? Grain jar [Re: tombosley8]
    #20587021 - 09/19/14 06:09 AM (10 years, 3 months ago)

If you wait a few days you will definitely find out if this is mold or bruising. Mold like to spread like wild fire once it appears but since you just shook could just be bruising.

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Re: Bruised myc or contamination? Grain jar [Re: motherchimp]
    #20587029 - 09/19/14 06:16 AM (10 years, 3 months ago)

Tyvek is more sketch than poly IMO. when your SFD's come you'll never look back.

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