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InvisibleOgla
losfreddy
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Re: thinking about cross breeding again [Re: prismism]
    #20584189 - 09/18/14 03:20 PM (10 years, 3 months ago)

Quote:

prismism said:
Quote:

cronicr said:
Quote:

prismism said:
I recently mixed half a syringe of PE and half a syringe of Chitwan. The results were some of the substrates fruited PE, and the others fruited Chitwan. Some of the subs fruited one on the first flush and the other on the next. There were no hybrids that I saw.



pe is tricky to cross and spores is actually the easiest way to do it.print one on top the other. pasty jas a tbread in the growlogs i got one in my journal




I'm not going to go searching through your journal so how about you post a link or the photo proof in this here thread. I was under the impression that mixing spores does not create hybrids. I even thought RR had to use snake venom or some shit to create his 'hybrid' varieties. I also don't see why placing a spore print on top of another spore print would be that much different, or more effective, than simply mixing the spores together in a liquid solution.
I'm not buying it.





look at the link i posted in the OP.. how can you deny that the redspore and mazatapec didnt cross when you have a spore print with black and red spores?  here, ill repost the pic incase the deleted photos dont pop up for yall


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Invisibleprismism
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Re: thinking about cross breeding again [Re: Ogla]
    #20584216 - 09/18/14 03:28 PM (10 years, 3 months ago)

Quote:

losfreddy said:
Quote:

prismism said:
Quote:

cronicr said:
Quote:

prismism said:
I recently mixed half a syringe of PE and half a syringe of Chitwan. The results were some of the substrates fruited PE, and the others fruited Chitwan. Some of the subs fruited one on the first flush and the other on the next. There were no hybrids that I saw.



pe is tricky to cross and spores is actually the easiest way to do it.print one on top the other. pasty jas a tbread in the growlogs i got one in my journal




I'm not going to go searching through your journal so how about you post a link or the photo proof in this here thread. I was under the impression that mixing spores does not create hybrids. I even thought RR had to use snake venom or some shit to create his 'hybrid' varieties. I also don't see why placing a spore print on top of another spore print would be that much different, or more effective, than simply mixing the spores together in a liquid solution.
I'm not buying it.





look at the link i posted in the OP.. how can you deny that the redspore and mazatapec didnt cross when you have a spore print with black and red spores?  here, ill repost the pic incase the deleted photos dont pop up for yall






Personally, I don't see black and red in those photos. I see some prints that could be considered rust colored, and some that could be considered purple. I would like to see a hybrid fruit. One that has distinguished characteristics of each variety that was crossed.


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ephemeral anomalous

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Invisiblemaddchef
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Re: thinking about cross breeding again [Re: prismism]
    #20584229 - 09/18/14 03:31 PM (10 years, 3 months ago)

The only distinguishable quality would be the spores.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

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Invisibleprismism
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Re: thinking about cross breeding again [Re: prismism]
    #20584232 - 09/18/14 03:32 PM (10 years, 3 months ago)

Well, that's no fun.


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ephemeral anomalous

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OfflineSh33p.Milk
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Re: thinking about cross breeding again [Re: prismism]
    #20584252 - 09/18/14 03:35 PM (10 years, 3 months ago)

Quote:

prismism said:
Quote:

Sh33p.Milk said:
Monokaryons or dikaryons can be crossed.  Snake venom is used for non-compatible strains.  There are other resources for this information on this website.




What determines whether or not two 'strains' of the same species are compatible or not?




That's where the fungi still blow my mind.  I believe that they need to be of different mating types.  Some are bipolar (two mating types) and some are tetrapolar (4+)
This is were I will stop before a TC blows me out of the water with their remarkable mushroom wisdom. 
I would read up on it though, I think it's super interesting!  :toast:

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OfflinecronicrMFacebook
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Re: thinking about cross breeding again [Re: Sh33p.Milk]
    #20584323 - 09/18/14 03:49 PM (10 years, 3 months ago)

im on my phone or i woild. venom is ussed for incompatible steains. most cubes wont need it but pe is one thatt does.
ill dig in more when im at my comp...try the search engine


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinecronicrMFacebook
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Re: thinking about cross breeding again [Re: cronicr]
    #20584343 - 09/18/14 03:53 PM (10 years, 3 months ago)

and there not hybrids.their both cubes


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisiblePastywhyteMDiscord
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Re: thinking about cross breeding again [Re: cronicr]
    #20584364 - 09/18/14 03:57 PM (10 years, 3 months ago)

Quote:

cronicr said:
im on my phone or i woild. venom is ussed for incompatible steains. most cubes wont need it but pe is one thatt does.
ill dig in more when im at my comp...try the search engine




Though workman never needed it when he made APE. . . that could be due to the fact that when he did it he had an entire jar colonized with monokarotic myc so any spore that germinated had no where else it could go if it wanted food.

A great read.

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5194890/page/0/fpart/all/vc/1

Edited by Pastywhyte (09/18/14 04:22 PM)

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OfflinecronicrMFacebook
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Re: thinking about cross breeding again [Re: Pastywhyte]
    #20584432 - 09/18/14 04:12 PM (10 years, 3 months ago)

love that thread! his whole journal is mindblowing lol!
since pris doesn't wanna search i'll do it:lol:
Snake venom isn't really needed for crossing strains of the same species. As anno said, they'll do that on their own. I've used it to cross non-viable spores with monokaryotic mycelium in order to get the DNA from the old spores, but that's not a process I'd recommend unless one is really experienced with lab work.  Snake venom also holds promise for crossing different species.  More work on that is ongoing.
RR

http://www.shroomery.org/forums/showflat.php/Number/8488033#8488033

Spores from different strains are totally compatible, just as pollen from bubblegum can easily cross with purple haze. There is no challenge or or anything special about 'man made' strains. It happens every time you squirt spores into a jar. A strain is made when compatible monokaryons cross paths and exchange genetic information. It mattes little what the 'name' on the print was, or even if the monokaryons came from separate prints. Dikaryons can easily cross too via anastomosis. You need to pick up that book and do some reading. It gives a great starting point to build from and will dispel your early misunderstandings.
RR

http://www.shroomery.org/forums/showflat.php/Number/6616102#6616102
Strains of the same species cross easily.  I avoid calling them 'hybrids', especially since the only difference many of the strains have is the name.  Every time you use multispore inoculation, thousands of 'strains' are created.  Most of these combine into a single organism.  Whether the spores came from the same print or multiple prints is irrelevant.
RR
i like this one, here's the next reply
Quote:

prismism said:
Quote:

RogerRabbit said:
Strains of the same species cross easily.  I avoid calling them 'hybrids', especially since the only difference many of the strains have is the name.  Every time you use multispore inoculation, thousands of 'strains' are created.  Most of these combine into a single organism.  Whether the spores came from the same print or multiple prints is irrelevant.
RR




QFT



http://www.shroomery.org/forums/showflat.php/Number/11270111#11270111
Any single spore which germinates forms monokaryotic mycelium.  Simply dilute the spore solution enough to swipe individual spores with a space around them so they're easy to grab.

An easier method is to simply make a print with one cap, and then set the cap from the other 'strain' on top of it, to print both caps on the same medium. Then use multispore inoculation and look later for the cross you were hoping for after fruits develop.
RR
http://www.shroomery.org/forums/showflat.php/Number/18934986#18934986


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflinecronicrMFacebook
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Re: thinking about cross breeding again [Re: cronicr]
    #20584435 - 09/18/14 04:13 PM (10 years, 3 months ago)

:uppercut:


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Offlinespacechildo
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Re: thinking about cross breeding again [Re: prismism]
    #20584447 - 09/18/14 04:15 PM (10 years, 3 months ago)

its really incredible how people can say things like
"I really wanna see it" and "I'm not buying it"
when all you have to do to both see and get it is click the small book under his name :shrug:

as cron said most cubes will mix, venom is for different species
or for hard to do strains (unless you're a wiz like wizWorkman..

Quote:

cronicr said:
:uppercut:




Indeed! :yesnod: :science:

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OfflinecronicrMFacebook
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Re: thinking about cross breeding again [Re: maddchef]
    #20584509 - 09/18/14 04:31 PM (10 years, 3 months ago)

Quote:

prismism said:
Quote:

cronicr said:
Quote:

prismism said:
I recently mixed half a syringe of PE and half a syringe of Chitwan. The results were some of the substrates fruited PE, and the others fruited Chitwan. Some of the subs fruited one on the first flush and the other on the next. There were no hybrids that I saw.



pe is tricky to cross and spores is actually the easiest way to do it.print one on top the other. pasty jas a tbread in the growlogs i got one in my journal




I'm not going to go searching through your journal so how about you post a link or the photo proof in this here thread. I was under the impression that mixing spores does not create hybrids. I even thought RR had to use snake venom or some shit to create his 'hybrid' varieties. I also don't see why placing a spore print on top of another spore print would be that much different, or more effective, than simply mixing the spores together in a liquid solution.
I'm not buying it.



i was actually only saying pe is tricky to cross and it normally needs snake venom, the rest of my post was directed at
Quote:

maddchef said:
Mixing spores doesn't do anything, usually you need the nuclei of each spore to interact.



that.


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisiblemaddchef
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Re: thinking about cross breeding again [Re: cronicr]
    #20584520 - 09/18/14 04:35 PM (10 years, 3 months ago)

When he said cross breeding I was assuming he meant crossing strains and not mixing cubes.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:

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OfflinecronicrMFacebook
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Re: thinking about cross breeding again [Re: maddchef]
    #20584531 - 09/18/14 04:37 PM (10 years, 3 months ago)

no worries i was just clearing the air that crossing cubes is simple for the most part, the trick is spotting it(be hard to cross a gt and b+ and know you got it right unless you used isolates)
nice to see ya chef


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisiblemaddchef
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Re: thinking about cross breeding again [Re: cronicr]
    #20584539 - 09/18/14 04:39 PM (10 years, 3 months ago)

:wink:


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:

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Offlineimjoe
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Re: thinking about cross breeding again [Re: maddchef]
    #20584544 - 09/18/14 04:40 PM (10 years, 3 months ago)

This seems like a cool idea, you should create a new log when you do it. Best of luck!

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InvisibleOgla
losfreddy
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Re: thinking about cross breeding again [Re: prismism]
    #20584698 - 09/18/14 05:11 PM (10 years, 3 months ago)

Quote:

prismism said:
Personally, I don't see black and red in those photos. I see some prints that could be considered rust colored, and some that could be considered purple. I would like to see a hybrid fruit. One that has distinguished characteristics of each variety that was crossed.






Bro, the link the OP. Here are pics of the hybrid fruit


Quote:

losfreddy said:
And today we have more grow and flush pics :smile:

Mazatapec




MazaRedspore, 3rd flush!



Since the mazatapec flushed at the same time as the mazaredspore, i decided that this would b a great opportunity to take side by side comparison pics.

To the left, mazarespore., right mazatapec.  Notice the mazaRedspores have a pointy nipple. They both look very similar with minor differences. Gills, and stipes r about the same.  Mazatapec has more prolific fruits, where as mazaRedspore is miniture compared to both parents.  Also to keep in mind that the MazaRedspore has redspores in it.






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OfflinecronicrMFacebook
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Re: thinking about cross breeding again [Re: Ogla]
    #20584722 - 09/18/14 05:14 PM (10 years, 3 months ago)

nice pics


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisibleprismism
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Re: thinking about cross breeding again [Re: cronicr]
    #20585144 - 09/18/14 06:50 PM (10 years, 3 months ago)

Quote:

cronicr said:
Quote:

RogerRabbit said:
Strains of the same species cross easily.  I avoid calling them 'hybrids', especially since the only difference many of the strains have is the name.  Every time you use multispore inoculation, thousands of 'strains' are created.  Most of these combine into a single organism.  Whether the spores came from the same print or multiple prints is irrelevant.
RR







Thank you for finding that quote. That is the point I have been basically trying to make. I've mixed spores on several occasions, and have never had a noticeable hybrid fruit. They have always retained the visual genetics of one or the other.
I suppose what you're saying is that to get a noticeable cross between two varying sets of genetics- you have to get lucky? Or that the only way you can tell if your genetics actually crossed is if the spore print has a specific color? I'm a little confused I must say.


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OfflinecronicrMFacebook
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Re: thinking about cross breeding again [Re: prismism]
    #20585192 - 09/18/14 07:02 PM (10 years, 3 months ago)

you should be using ones you can easily tell apart like pasty did with AA+ and rust spores, he was shooting for the albino look with red spores but got a normal colored cube with purple/black spores lol!
i treid the same thing and came pretty friggin close but i did the print on print method but still wasn't totally luecistic

but you can however see the rust spores


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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