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Offlinemud guppy
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Still Air Box
    #20573765 - 09/16/14 01:46 AM (9 years, 4 months ago)

hi i see and hear and read alot of people talking about flow hoods etc! well i started building one then turned it into a owl box and found the brilliant idea of a still air box! works very well modified to suit me and ive only had 1 contamination so far but it was actually from a petri dish that had trich in! peoples thoughts?


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Offlineant707
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Registered: 07/26/14
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Re: Still Air Box [Re: mud guppy]
    #20573833 - 09/16/14 02:53 AM (9 years, 4 months ago)

to me....a flow hood is something to work towards. a lot of peeps here use SABs with great results.

while SABs are confining and a PITA, i really dont have room for a flow hood.

practice your sterile technique and your results will improve

edit: im not sure this is really "advanced" mycology ???


Edited by ant707 (09/16/14 02:54 AM)


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OfflineMrcloudy
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Re: Still Air Box [Re: ant707]
    #20573957 - 09/16/14 04:57 AM (9 years, 4 months ago)

Still air boxes are terrible in my opinion and I only use one out of necessity. Flowhoods are much better and easier to work with. My contamination rate has gone up a bit, but I have also become a bit careless. It's kind of hard to keep the same precision when your movement is limited. Pretty good for agar to grain though I guess, never had a contamination due to using the SAB, incomplete sterilization of the grain is what did me in. Pouring agar is a major pain. Trying to see through the plastic to take tissue samples is ridiculous. Flowhood all the way, SAB are ok out of necessity but yeah a flowhood should be a goal for anyone who wants to really delve deeper into the hobby.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflinePussyFart
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Re: Still Air Box [Re: Mrcloudy]
    #20574296 - 09/16/14 08:18 AM (9 years, 4 months ago)

Quote:

Mrcloudy said:
Still air boxes are terrible in my opinion and I only use one out of necessity. Flowhoods are much better and easier to work with.



I only use SABs and have a 99-100% success rate.....I have no plans on building a flow hood......ever.....

If the air is still, it is impossible for a contam to blow on your work.

If you are getting contams with a SAB, but not with a flow hood, your sterile technique is probably to blame.

There is nothing that can be done in front of a flow hood that cannot be done in a SAB with the exact same success rate.

If your SAB is too small and too much of a PITA to work with, maybe you should build a bigger one....

Quote:

Mrcloudy said:
a flowhood should be a goal for anyone who wants to really delve deeper into the hobby.



I have gone pretty deep.......still don't see a need for a LFH.

   


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OfflinePsyDragon
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Registered: 10/21/05
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Re: Still Air Box [Re: PussyFart]
    #20574342 - 09/16/14 08:33 AM (9 years, 4 months ago)

I definitely think it helps to isolate the room where you work and have a HEPA filter running recirculate most of the time to keep a lower ambient of potential contaminates in the space to begin with. Whether that, coupled with sterile technique, is enough ... YMMV. I get away with it doing pftek, but probably would take more precautions with bulk, lotta eggs in one basket there.


Edited by ColorDragon (09/16/14 08:34 AM)


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OfflineSh33p.Milk
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Registered: 09/13/14
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Re: Still Air Box [Re: PsyDragon]
    #20575813 - 09/16/14 03:37 PM (9 years, 4 months ago)

I agree with ColorDragon's info.  My experience was strange in that I first began working under a flow hood, and then went through an era where I was left with nothing but a SAB.
I found that having no option other than to be VERY mindful about my sterile technique made the process more rewarding  in the end. 
There is a lot of value in being able to make due with poopy make-shift equipment IMHO :thumbup:


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OfflineMrcloudy
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Re: Still Air Box [Re: Sh33p.Milk]
    #20576122 - 09/16/14 05:01 PM (9 years, 4 months ago)

Quote:

Sh33p.Milk said:

There is a lot of value in being able to make due with poopy make-shift equipment IMHO :thumbup:




I will say that it has made my sterile procedure that much better.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflineKizzle
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Re: Still Air Box [Re: Mrcloudy]
    #20580520 - 09/17/14 05:22 PM (9 years, 4 months ago)

Quote:

It's kind of hard to keep the same precision when your movement is limited.



Then make one that doesn't limit your movement:cookiemonster:


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InvisibleJuiceh
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Re: Still Air Box [Re: Kizzle]
    #20583472 - 09/18/14 12:08 PM (9 years, 4 months ago)

Flowhood all the way. I built a SAB a similar size to the one PussyFart posted, but taller. Still not a fan. The workflow with a SAB is far too slow when you have a lot of work to do. Sure you can use a SAB to g2g a dozen jars in a somewhat reasonable amount of time. But when you have to do multiple dozens of jars and Large substrate bags AND heat seal the bags a SAB just doesn't cut it.

When I used a SAB my contam rate was fairly low. Now with my 4'x2' flow hood it's nonexistent. The only contams I've seen were from bad LC syringes from a vendor. I can leave an open petri dish in front of my flow hood for over a day and it won't get contaminated. Theres a thread on here where someone tested the amount of time they can leave petris open in their SAB. I believe they couldn't get away with having the dish open for more than a couple minutes without them getting contamed.

Nowadays when I use my SAB, I only use it for cleaning up dirty cultures on agar. I also no longer prep my SAB like most people do. I don't chem bomb my SAB with lysol like some do. It is prepped in front of the running flow hood. The SAB is simply wiped down with alcohol and then put up against the flow hood to blast the filtered air through it for about 5 minutes. While this is happening, in front of the hood, I wipe down whatever is to go into the SAB with alcohol. Then I tip the SAB over and place everything it it and close it up, all while still in front of the flow hood. Then i shut off the flow hood and I do not waste any time after that waiting for the air to settle in the box. That air is already very clean and is settled enough before the work begins. Works much better this way when I need to use it.

If you can have both, do it. They each have their place in this hobby.


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Edited by Juiceh (09/18/14 12:20 PM)


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InvisibleJuiceh
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Re: Still Air Box [Re: Kizzle]
    #20583563 - 09/18/14 12:37 PM (9 years, 4 months ago)

Quote:

Kizzle said:
Quote:

It's kind of hard to keep the same precision when your movement is limited.



Then make one that doesn't limit your movement:cookiemonster:



Heres a statement from Pussyfart about his own SAB in his SAB thread.
Quote:

PussyFart said:I actually don't really like it because it's not tall enough to do a grain to grain transfers comfortably...




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Trade List

How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
RogerRabbit said:You need a bigger pressure cooker for this hobby. RR


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OfflinePussyFart
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Re: Still Air Box [Re: Juiceh]
    #20583584 - 09/18/14 12:44 PM (9 years, 4 months ago)

Just because I don't like it, does not mean it doesn't work......being a little taller would help a bit, but I manage just fine.


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OfflineKizzle
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Re: Still Air Box [Re: PussyFart]
    #20584744 - 09/18/14 05:19 PM (9 years, 4 months ago)

Quote:

Flowhood all the way. I built a SAB a similar size to the one PussyFart posted, but taller. Still not a fan. The workflow with a SAB is far too slow when you have a lot of work to do. Sure you can use a SAB to g2g a dozen jars in a somewhat reasonable amount of time. But when you have to do multiple dozens of jars and Large substrate bags AND heat seal the bags a SAB just doesn't cut it.



It doesn't have to be that way. I mean there's nothing wrong with preferring a flow hood better I just want to make it clear that the problems you're mentioning aren't inherent to all SAB designs. I can easily load and unload jars in mine without significantly disturbing the upper still air layer where the actual sterile work is performed although that's usually not necessary.


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