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OfflineSuspiciousShroomer
First Time Grower
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Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
Will jar heat ruin my spores?
    #20579862 - 09/17/14 02:45 PM (10 years, 3 months ago)

So I sterilized my jars (4 jars) and I let them cool for 20 minutes before moving to a glove box. They weren't hot enough not to hold but hot enough to make my hands uncomfortable.

Will this ruin the spores or should I be okay?

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Offlinepawnzy
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Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
Re: Will jar heat ruin my spores? [Re: SuspiciousShroomer]
    #20579888 - 09/17/14 02:52 PM (10 years, 3 months ago)

You should let them cool to room temp completely.
Spores are not thermotolerant, as another recent thread describes, however im not sure exactly how hot is too hot for inoculation.

Cant wait to see some answers

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OfflineSuspiciousShroomer
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Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
Re: Will jar heat ruin my spores? [Re: pawnzy]
    #20579898 - 09/17/14 02:55 PM (10 years, 3 months ago)

It's too late now. I already put the spores in. I'm kind of pissed at myself, I made a couple mistakes that could have been avoided.

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Offlinepawnzy
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Re: Will jar heat ruin my spores? [Re: SuspiciousShroomer]
    #20579906 - 09/17/14 02:56 PM (10 years, 3 months ago)

Quote:

SuspiciousShroomer said:
It's too late now. I already put the spores in. I'm kind of pissed at myself, I made a couple mistakes that could have been avoided.



like what? other than injecting hot jars. hope that wasn't your last syringe

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OfflineFlomasta100
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Registered: 08/25/14
Posts: 167
Last seen: 10 years, 2 months
Re: Will jar heat ruin my spores? [Re: pawnzy]
    #20579927 - 09/17/14 03:01 PM (10 years, 3 months ago)

You can probably sterilize again (to be on the safe side), let them cool for 8 hours and inoculate again. You may have ruined the moisture content though by not letting them cool "slowly" by leaving them in the sterilizer.

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OfflineSuspiciousShroomer
First Time Grower
Male


Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
Re: Will jar heat ruin my spores? [Re: pawnzy]
    #20579942 - 09/17/14 03:03 PM (10 years, 3 months ago)

It very much was. I'll have to order another one.

I put the spores in when the jars were a little hot. (Under 100 degrees, probably around 90 maybe less)

I didn't sterilize the needle between each jar.

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OfflineFlomasta100
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Re: Will jar heat ruin my spores? [Re: SuspiciousShroomer]
    #20579953 - 09/17/14 03:05 PM (10 years, 3 months ago)

I would keep them just in case you didn't kill the spores. You probably did though... :/

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OfflineSuspiciousShroomer
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Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
Re: Will jar heat ruin my spores? [Re: Flomasta100]
    #20579975 - 09/17/14 03:10 PM (10 years, 3 months ago)

Fingers crossed. I won't be pissed. I'll do it much better next time if these don't work. If they do, then there is a God.

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Offlinepawnzy
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Re: Will jar heat ruin my spores? [Re: SuspiciousShroomer]
    #20579996 - 09/17/14 03:13 PM (10 years, 3 months ago)

There is no god here, other than mushrooms.

You probably wont get to meet him either.

if anything one jar will make it (the first one) and the rest will cross contaminate.

You might get lucky, but i dont believe in luck either and i think you should do some cramming on the reading aspect of this hobby and work on your sterile technique.

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OfflineSuspiciousShroomer
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Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
Re: Will jar heat ruin my spores? [Re: pawnzy]
    #20580004 - 09/17/14 03:15 PM (10 years, 3 months ago)

I was very sterile. I just got caught up in excitement. I have a really good glove box. I have a really good sterilized spare room. I only use gloves and a mask to touch anything to do with the jars or spores. I flame sterilized and alcohol sterilized the needle. Room is scrubbed in bleach day before this.

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OfflinecronicrMFacebook
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Re: Will jar heat ruin my spores? [Re: SuspiciousShroomer]
    #20580020 - 09/17/14 03:19 PM (10 years, 3 months ago)

dam dude, you really should just follow the tek to a T next time and i'll tell ya what, if you promise to do so i will send you a print to work with and help you out along your journey, never wipe the needle with alcohol it doesn't sterilize it only sanitizes


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineSuspiciousShroomer
First Time Grower
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Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
Re: Will jar heat ruin my spores? [Re: cronicr]
    #20580032 - 09/17/14 03:21 PM (10 years, 3 months ago)

So you think I'm fucked?

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Offlinepawnzy
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Re: Will jar heat ruin my spores? [Re: cronicr]
    #20580054 - 09/17/14 03:25 PM (10 years, 3 months ago)

Quote:

cronicr said:
dam dude, you really should just follow the tek to a T next time and i'll tell ya what, if you promise to do so i will send you a print to work with and help you out along your journey, never wipe the needle with alcohol it doesn't sterilize it only sanitizes




Yo take this offer dude, seriously.

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Offlinepawnzy
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Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
Re: Will jar heat ruin my spores? [Re: SuspiciousShroomer]
    #20580058 - 09/17/14 03:26 PM (10 years, 3 months ago)

Quote:

SuspiciousShroomer said:
So you think I'm fucked?




Does the pope rape kids?

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OfflineSuspiciousShroomer
First Time Grower
Male


Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
Re: Will jar heat ruin my spores? [Re: pawnzy]
    #20580068 - 09/17/14 03:28 PM (10 years, 3 months ago)

Okay guys, update, I might be safe. The jars after 20 minutes are only 79 degrees in the middle. I sanitized a thermometer to check.

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Offlinepawnzy
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Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
Re: Will jar heat ruin my spores? [Re: SuspiciousShroomer]
    #20580081 - 09/17/14 03:31 PM (10 years, 3 months ago)

Quote:

SuspiciousShroomer said:
Okay guys, update, I might be safe. The jars after 20 minutes are only 79 degrees in the middle. I sanitized a thermometer to check.




So you stuck a dirty ol thermometer in a jar thats already inoculated? did you flame sterilize?  :willynilly:

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OfflineSuspiciousShroomer
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Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
Re: Will jar heat ruin my spores? [Re: pawnzy]
    #20580088 - 09/17/14 03:33 PM (10 years, 3 months ago)

I flame sterilized and let cool in a clean paper towel.

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Offlinepawnzy
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Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
Re: Will jar heat ruin my spores? [Re: SuspiciousShroomer]
    #20580115 - 09/17/14 03:41 PM (10 years, 3 months ago)

paper towel aint clean, but k you inoculated now do nothing for 2 weeks man just be patient, no more thermometers and changing variables. in the mean time get another syringe  / take cronicrs offer which i highly suggest. hes a tc, which stands for knows your fuckin stuff, very active and willing obviously to teach you,

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OfflineSuspiciousShroomer
First Time Grower
Male


Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
Re: Will jar heat ruin my spores? [Re: pawnzy]
    #20580121 - 09/17/14 03:43 PM (10 years, 3 months ago)

I'm just going to be patient and if nothing happens in 2 weeks, I will start over.

And yes I will probably take him up on his offer. Free spores and free tutoring ain't nothing to turn down.

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OfflineFlomasta100
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Registered: 08/25/14
Posts: 167
Last seen: 10 years, 2 months
Re: Will jar heat ruin my spores? [Re: cronicr]
    #20580251 - 09/17/14 04:16 PM (10 years, 3 months ago)

Quote:

cronicr said:
dam dude, you really should just follow the tek to a T next time and i'll tell ya what, if you promise to do so i will send you a print to work with and help you out along your journey, never wipe the needle with alcohol it doesn't sterilize it only sanitizes




Since you're handing out prints... LOL jk

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Shop: North Spore Injection Grain Bag   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures


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