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SuspiciousShroomer
First Time Grower



Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
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Will jar heat ruin my spores?
#20579862 - 09/17/14 02:45 PM (10 years, 3 months ago) |
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So I sterilized my jars (4 jars) and I let them cool for 20 minutes before moving to a glove box. They weren't hot enough not to hold but hot enough to make my hands uncomfortable.
Will this ruin the spores or should I be okay?
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pawnzy
That Guy


Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
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You should let them cool to room temp completely. Spores are not thermotolerant, as another recent thread describes, however im not sure exactly how hot is too hot for inoculation.
Cant wait to see some answers
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SuspiciousShroomer
First Time Grower



Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
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Re: Will jar heat ruin my spores? [Re: pawnzy]
#20579898 - 09/17/14 02:55 PM (10 years, 3 months ago) |
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It's too late now. I already put the spores in. I'm kind of pissed at myself, I made a couple mistakes that could have been avoided.
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pawnzy
That Guy


Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
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Quote:
SuspiciousShroomer said: It's too late now. I already put the spores in. I'm kind of pissed at myself, I made a couple mistakes that could have been avoided.
like what? other than injecting hot jars. hope that wasn't your last syringe
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Flomasta100
Avid Apprentice

Registered: 08/25/14
Posts: 167
Last seen: 10 years, 2 months
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Re: Will jar heat ruin my spores? [Re: pawnzy]
#20579927 - 09/17/14 03:01 PM (10 years, 3 months ago) |
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You can probably sterilize again (to be on the safe side), let them cool for 8 hours and inoculate again. You may have ruined the moisture content though by not letting them cool "slowly" by leaving them in the sterilizer.
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SuspiciousShroomer
First Time Grower



Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
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Re: Will jar heat ruin my spores? [Re: pawnzy]
#20579942 - 09/17/14 03:03 PM (10 years, 3 months ago) |
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It very much was. I'll have to order another one.
I put the spores in when the jars were a little hot. (Under 100 degrees, probably around 90 maybe less)
I didn't sterilize the needle between each jar.
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Flomasta100
Avid Apprentice

Registered: 08/25/14
Posts: 167
Last seen: 10 years, 2 months
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I would keep them just in case you didn't kill the spores. You probably did though... :/
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SuspiciousShroomer
First Time Grower



Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
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Re: Will jar heat ruin my spores? [Re: Flomasta100]
#20579975 - 09/17/14 03:10 PM (10 years, 3 months ago) |
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Fingers crossed. I won't be pissed. I'll do it much better next time if these don't work. If they do, then there is a God.
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pawnzy
That Guy


Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
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There is no god here, other than mushrooms.
You probably wont get to meet him either.
if anything one jar will make it (the first one) and the rest will cross contaminate.
You might get lucky, but i dont believe in luck either and i think you should do some cramming on the reading aspect of this hobby and work on your sterile technique.
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SuspiciousShroomer
First Time Grower



Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
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Re: Will jar heat ruin my spores? [Re: pawnzy]
#20580004 - 09/17/14 03:15 PM (10 years, 3 months ago) |
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I was very sterile. I just got caught up in excitement. I have a really good glove box. I have a really good sterilized spare room. I only use gloves and a mask to touch anything to do with the jars or spores. I flame sterilized and alcohol sterilized the needle. Room is scrubbed in bleach day before this.
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cronicr



Registered: 08/07/11
Posts: 62,899
Loc: Van Isle
Last seen: 2 hours, 59 minutes
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dam dude, you really should just follow the tek to a T next time and i'll tell ya what, if you promise to do so i will send you a print to work with and help you out along your journey, never wipe the needle with alcohol it doesn't sterilize it only sanitizes
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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SuspiciousShroomer
First Time Grower



Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
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Re: Will jar heat ruin my spores? [Re: cronicr]
#20580032 - 09/17/14 03:21 PM (10 years, 3 months ago) |
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So you think I'm fucked?
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pawnzy
That Guy


Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
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Re: Will jar heat ruin my spores? [Re: cronicr]
#20580054 - 09/17/14 03:25 PM (10 years, 3 months ago) |
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Quote:
cronicr said: dam dude, you really should just follow the tek to a T next time and i'll tell ya what, if you promise to do so i will send you a print to work with and help you out along your journey, never wipe the needle with alcohol it doesn't sterilize it only sanitizes
Yo take this offer dude, seriously.
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pawnzy
That Guy


Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
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Quote:
SuspiciousShroomer said: So you think I'm fucked?
Does the pope rape kids?
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SuspiciousShroomer
First Time Grower



Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
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Re: Will jar heat ruin my spores? [Re: pawnzy]
#20580068 - 09/17/14 03:28 PM (10 years, 3 months ago) |
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Okay guys, update, I might be safe. The jars after 20 minutes are only 79 degrees in the middle. I sanitized a thermometer to check.
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pawnzy
That Guy


Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
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Quote:
SuspiciousShroomer said: Okay guys, update, I might be safe. The jars after 20 minutes are only 79 degrees in the middle. I sanitized a thermometer to check.
So you stuck a dirty ol thermometer in a jar thats already inoculated? did you flame sterilize?
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SuspiciousShroomer
First Time Grower



Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
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Re: Will jar heat ruin my spores? [Re: pawnzy]
#20580088 - 09/17/14 03:33 PM (10 years, 3 months ago) |
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I flame sterilized and let cool in a clean paper towel.
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pawnzy
That Guy


Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
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paper towel aint clean, but k you inoculated now do nothing for 2 weeks man just be patient, no more thermometers and changing variables. in the mean time get another syringe / take cronicrs offer which i highly suggest. hes a tc, which stands for knows your fuckin stuff, very active and willing obviously to teach you,
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SuspiciousShroomer
First Time Grower



Registered: 09/14/14
Posts: 22
Loc: Indiana
Last seen: 10 years, 3 months
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Re: Will jar heat ruin my spores? [Re: pawnzy]
#20580121 - 09/17/14 03:43 PM (10 years, 3 months ago) |
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I'm just going to be patient and if nothing happens in 2 weeks, I will start over.
And yes I will probably take him up on his offer. Free spores and free tutoring ain't nothing to turn down.
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Flomasta100
Avid Apprentice

Registered: 08/25/14
Posts: 167
Last seen: 10 years, 2 months
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Re: Will jar heat ruin my spores? [Re: cronicr]
#20580251 - 09/17/14 04:16 PM (10 years, 3 months ago) |
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Quote:
cronicr said: dam dude, you really should just follow the tek to a T next time and i'll tell ya what, if you promise to do so i will send you a print to work with and help you out along your journey, never wipe the needle with alcohol it doesn't sterilize it only sanitizes
Since you're handing out prints... LOL jk
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StAlfonso
Student of mycology

Registered: 09/04/14
Posts: 4
Loc: Mpls MN
Last seen: 9 years, 7 months
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Re: Will jar heat ruin my spores? [Re: pawnzy]
#20580373 - 09/17/14 04:46 PM (10 years, 3 months ago) |
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Patience is a virtue.... Especially in cultivating plants and fungus.
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