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InvisibleGhatti
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Registered: 06/23/14
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Agar timeframe Q
    #20579243 - 09/17/14 11:44 AM (10 years, 3 months ago)

If I'm testing air samples.......

So I opened several plates for 10 seconds to test the quality of the air I am using. At 75ish degrees how long would you guess before I will see growth on 250ml h2o, 5g agar, 6g LME?

I know it depends on what lands on it and such, just looking for a ballpark.

Would you call it safe if nothing shows up for 5 days or do I need more?

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InvisibleHyphai
Registered: 04/03/14
Posts: 517
Re: Agar timeframe Q [Re: Ghatti]
    #20579248 - 09/17/14 11:46 AM (10 years, 3 months ago)

I wouldn't do that at all. Sounds like a good way to waste supplies though.  Why would you want to slack on your aseptic technique?

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OfflinePussyFart
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Re: Agar timeframe Q [Re: Ghatti]
    #20579249 - 09/17/14 11:46 AM (10 years, 3 months ago)

Quote:

Ghatti said:
If I'm testing air samples.......

So I opened several plates for 10 seconds to test the quality of the air I am using. At 75ish degrees how long would you guess before I will see growth on 250ml h2o, 5g agar, 6g LME?

I know it depends on what lands on it and such, just looking for a ballpark.

Would you call it safe if nothing shows up for 5 days or do I need more?



I would give it a week(maybe 2 to be safe) and if nothing by then you should be good.....but it could very well take longer.....I have seen plates take a month to "go bad" from open exposure to the elements.

Edited by PussyFart (09/17/14 11:46 AM)

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InvisibleGhatti
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Re: Agar timeframe Q [Re: PussyFart]
    #20579272 - 09/17/14 11:50 AM (10 years, 3 months ago)

Why would I open a blank plate to test the quality of filtered air????

Because its nice to know if your filter is efficient, what junk you have in your house, etc.

Agar is cheap, petris are cheap, etc.

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OfflineFelixDaCat
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Re: Agar timeframe Q [Re: Ghatti]
    #20579302 - 09/17/14 11:56 AM (10 years, 3 months ago)

I don't understand your concern Hyphai. If he wants to conduct an experiment at his expense he should be able to do so without being judged. Its not wasting supplies if he has a legitimate reason.

Edited by FelixDaCat (09/18/14 02:08 AM)

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InvisibleGhatti
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Re: Agar timeframe Q [Re: FelixDaCat]
    #20579318 - 09/17/14 11:58 AM (10 years, 3 months ago)

That's actually part of maintaining aseptic technique, ensuring the quality of your "sterile" air is up to par.

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OfflinePussyFart
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Re: Agar timeframe Q [Re: Ghatti]
    #20579320 - 09/17/14 12:00 PM (10 years, 3 months ago)

I use "control" dishes all the time when I do work in my SAB......it's not a waste to me.....

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Offlinepawnzy
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Re: Agar timeframe Q [Re: PussyFart]
    #20579506 - 09/17/14 12:45 PM (10 years, 3 months ago)

Quote:

PussyFart said:
Quote:

Ghatti said:
If I'm testing air samples.......

So I opened several plates for 10 seconds to test the quality of the air I am using. At 75ish degrees how long would you guess before I will see growth on 250ml h2o, 5g agar, 6g LME?

I know it depends on what lands on it and such, just looking for a ballpark.

Would you call it safe if nothing shows up for 5 days or do I need more?



I would give it a week(maybe 2 to be safe) and if nothing by then you should be good.....but it could very well take longer.....I have seen plates take a month to "go bad" from open exposure to the elements.




How interesting...

Crazy that open air can take that long in comparison to noccing up and seeing contams in days, even with the presence of healthy myc.

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InvisiblePastywhyteMDiscord
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Re: Agar timeframe Q [Re: pawnzy]
    #20579613 - 09/17/14 01:16 PM (10 years, 3 months ago)

Lots of variables.  I also like to test grain jars as well, see if the filters are still working, etc. Never hurts to test stuff :thumbup:

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OfflinecronicrMFacebook
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Re: Agar timeframe Q [Re: Pastywhyte]
    #20579663 - 09/17/14 01:30 PM (10 years, 3 months ago)

yep everytime i've set something new up like a new sab i always do some quality control


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisibleGhatti
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Re: Agar timeframe Q [Re: cronicr]
    #20579803 - 09/17/14 02:30 PM (10 years, 3 months ago)

And we all agree if nothing shows in 10-14 days its probably safe to use?

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OfflinecronicrMFacebook
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Re: Agar timeframe Q [Re: Ghatti]
    #20579805 - 09/17/14 02:31 PM (10 years, 3 months ago)

i never actually use my control plates, i just assume my sab is in good working order lol


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinepawnzy
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Re: Agar timeframe Q [Re: cronicr]
    #20579834 - 09/17/14 02:39 PM (10 years, 3 months ago)

yeah by then you might as well pc them again because something can be laying dormant inside

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InvisibleGhatti
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Re: Agar timeframe Q [Re: pawnzy]
    #20579961 - 09/17/14 03:07 PM (10 years, 3 months ago)

With grain maybe but not petris I wouldn't think.

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Offlinepawnzy
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Re: Agar timeframe Q [Re: Ghatti]
    #20580084 - 09/17/14 03:31 PM (10 years, 3 months ago)

neither would i, but then again i wouldnt think half the shit i learned lol

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