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niteowl
GrandPaw
Registered: 07/01/03
Posts: 16,291
Loc:
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growing wild shrooms????
#2056353 - 10/30/03 04:49 AM (20 years, 5 months ago) |
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What would be the best(least contam)way to grow some of the wild mushrooms that I have been finding?
I have taken prints from them and usually have contams. Some one said that I could clone the live shroom with less chance of geting contams.
Does anyone here have any experience working with a wild strain?
Is their a cleaner way of making the syringe from the print?
Here is a link to my grow log http://www.shroomery.org/forums/showflat...&PHPSESSID=
It tells in more detail how I have been doing my prints and making the syringe.
-------------------- Live for the moment you are in nowDon't be bogged down by your pastDon't be afraid of what lies in your future
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 18 days, 22 hours
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Re: growing wild shrooms???? [Re: niteowl]
#2056359 - 10/30/03 04:56 AM (20 years, 5 months ago) |
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> Some one said that I could clone the live shroom with less chance of getting contams.
This is very much possible, yes.
Check http://www.shroomery.org/index/par/23462 and http://www.shroomery.org/index/par/23544 in general.
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Seraph
ShroomiN'SeraphiM
Registered: 06/23/02
Posts: 183
Loc: South Flordia
Last seen: 5 years, 10 months
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Re: growing wild shrooms???? [Re: Anno]
#2056529 - 10/30/03 07:52 AM (20 years, 5 months ago) |
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have u tried agar inoculate with the spores and then cut small pieces of uncontaminated mycelium.
and like anno said cloning could work as well. Im sure the cloning would be much easier but if it doesnt work agar would be you next step.
-------------------- (\(\ ( -.-) o_(")(") ~nicole
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niteowl
GrandPaw
Registered: 07/01/03
Posts: 16,291
Loc:
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Re: growing wild shrooms???? [Re: Seraph]
#2056726 - 10/30/03 09:51 AM (20 years, 4 months ago) |
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I am VERY new at this trying to grow shrooms. All I have ever done is the PF tek. I have never used agar, dont know what it is.
I have been making the print and syringe at home due to a cash flow deficit. Every time I think I will have enuf cash to order a syringe something comes up and takes my cash!
So Im stuck trying to gro with these wild cubes.
-------------------- Live for the moment you are in nowDon't be bogged down by your pastDon't be afraid of what lies in your future
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thisone
the one theydon't see
Registered: 11/06/02
Posts: 818
Last seen: 19 years, 17 hours
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Re: growing wild shrooms???? [Re: niteowl]
#2056772 - 10/30/03 10:12 AM (20 years, 4 months ago) |
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-------------------- Everything written above this line is completely false. I am terribly bored and write here to pass time. I do not participate or support any illegal activity.
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student
Stranger
Registered: 09/26/03
Posts: 137
Last seen: 19 years, 3 months
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Re: growing wild shrooms???? [Re: thisone]
#2056797 - 10/30/03 10:28 AM (20 years, 4 months ago) |
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I am too lazy right now to find a link, but check out Joshua's grow log for his USVI. It is very instructive.
good luck
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Werecat
Werecat
Registered: 09/25/03
Posts: 64
Loc: Portland, OR
Last seen: 16 years, 5 months
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Re: growing wild shrooms???? [Re: student]
#2056809 - 10/30/03 10:36 AM (20 years, 4 months ago) |
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In my experience cloning works better than going from a wild spore print as far as limiting contamination. I have had good luck though with spore prints taken carefully then streaked onto agar. With a bit of luck any contam's are limited and you can later isolate some clean mycellium and drop it into a clean dish.
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Fungi_x
Firekeeper of OTD
Registered: 08/17/03
Posts: 8,960
Loc: Michigan
Last seen: 3 years, 4 months
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Re: growing wild shrooms???? [Re: Werecat]
#2057013 - 10/30/03 12:10 PM (20 years, 4 months ago) |
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FSRE
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Ekstaza
stranger than most
Registered: 04/10/03
Posts: 4,324
Loc: Around the corner
Last seen: 11 months, 20 days
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Re: growing wild shrooms???? [Re: Fungi_x]
#2057303 - 10/30/03 01:41 PM (20 years, 4 months ago) |
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The only way that I can get spores is to take prints from wild cubes. I started my first grow with wild spore prints before I even found the shroomery. Sure, I had some contam issues, but I overcame them. I just don't see how it could be that difficult to take a relatively clean spore print from a wild cube. The spores that I started with came from mushrooms that were picked and then thrown in a bag with other mushrooms(not the smartest idea, but...). My point is that maybe(I know that I have no idea how clean you are) you should re-think your preparation and sterilization techniques. If I can do this then anyone can. I'm the laziest mofo there is.
Anyway, Good Luck on your future attempts.
-------------------- YOUR EXPERIENCE WITH ANY GIVEN DRUG ISN'T THE DEFINITIVE MEASURE OF THE DRUGS EFFECTS.
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niteowl
GrandPaw
Registered: 07/01/03
Posts: 16,291
Loc:
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Re: growing wild shrooms???? [Re: Ekstaza]
#2059729 - 10/31/03 02:20 AM (20 years, 4 months ago) |
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Im going to try "LiL KuSsH's Syringe Making Tek" this time and see how it works.
-------------------- Live for the moment you are in nowDon't be bogged down by your pastDon't be afraid of what lies in your future
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DMJ
creepyatmosphere
Registered: 10/10/03
Posts: 233
Loc: Dungeon
Last seen: 14 years, 8 months
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Re: growing wild shrooms???? [Re: niteowl]
#2059947 - 10/31/03 05:58 AM (20 years, 4 months ago) |
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Still no luck with that? I don't know if it's too cold there or not, but try making the print outside. I also don't know that making a syringe is necessary. Although many may disagree using re-hydration as their reason, BUT, you can innoculate directly from the print. Sterility plays a bigger issue, maybe, but oh well. As for hydration, if it's a relatively fresh print, no worries, but according to some Teks in The Shroomery, you can scrape directly off the print, and your substrate will re-hydarate them.
Oh, and like Ekstaza said, I am also a lazy motherfucker. Take a shower, alcohol wash the hands, spray some lysol(no name actually), turn on the oven. I use an alcohol lamp for the syringe. This is as far as I've ever went with sterility. Personally, I think the whole doctor suit, and Decontamination chamber is for large scale hobbies. And also to instill paranoia into you, so that you don't forget, or at least attempt to be clean. Thinking of this, I also handle my colonizing jars frequently, with dirty hands, while smoking, blah, blh, blah, never a contam. If you are still having probs with them, I would suggest Spring Cleaning your pad! As I am a clean freak, I guess maybe that's why I've had good luck with my lax Teks.
Peace
-------------------- I come alive in the darkness.
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