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InvisibleNumeroEno
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Re: About to start working with agar. [Re: MudaFuka]
    #20567149 - 09/14/14 04:23 PM (10 years, 4 months ago)

Fuck yeah. I was thinking about making some liquid inoculant using your tek since my blender fits pint jars. Does that keep longer since it's liquid? I have some syringe filter and SHIP lids that I got for LC, but every attempt of mine at making LC failed miserably. The LI like you make it seems really awesome. I figure by the time this PE is ready to go I'll have a few more grows under my belt. Kind of off topic, but does anyone know how common albino fruits are with PE uncut? I'd love to make an isolate from one.


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:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisibleMudaFuka
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Re: About to start working with agar. [Re: NumeroEno]
    #20567428 - 09/14/14 05:28 PM (10 years, 4 months ago)

I have never tried storing LI I use it the second I make it. If you want long term storage make slants.


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AMU
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InvisibleNumeroEno
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Re: About to start working with agar. [Re: MudaFuka]
    #20567570 - 09/14/14 06:06 PM (10 years, 4 months ago)

I've read a little about slants, but that seems tricky as hell, keeping the test tube at a 45 degree angle and pouring freshly PC'd agar into them. Do you need a special breathable plug for the test tubes, or is the air in them enough?


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisiblePastywhyteMDiscord
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Re: About to start working with agar. [Re: NumeroEno]
    #20567596 - 09/14/14 06:12 PM (10 years, 4 months ago)

No you pre pour the slant then PC with the lids on loose. Then pull outta the PC hot and in the SAB remove from the jar or whatever you PC'd them in and let cool on a angle. Its easier than it sounds.

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OfflinecronicrMFacebook
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Re: About to start working with agar. [Re: Pastywhyte]
    #20567607 - 09/14/14 06:15 PM (10 years, 4 months ago)

:whathesaid: just remember to eventually use them or it becomes a habbit worse then baseball and hockey cards as a kid:lol:


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InvisiblePastywhyteMDiscord
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Re: About to start working with agar. [Re: cronicr]
    #20567704 - 09/14/14 06:37 PM (10 years, 4 months ago)

Fuck no doubt,  I have become a print and slant junkie.  Just finished getting all my edibles in masters and am looking at the 2 dozen actives that need to be transferred over and shuddering.

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InvisibleMudaFuka
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Re: About to start working with agar. [Re: cronicr]
    #20567712 - 09/14/14 06:37 PM (10 years, 4 months ago)

That's why I just use petries and every few months I take them out and make transfers from the ones I want to keep growing and just toss out the rest. I get really frustrated and overwhelmed when I have too many things going on so I'm really trying to limit myself to no more than 4 or 5 cultures going at a time.


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AMU
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InvisibleNumeroEno
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Re: About to start working with agar. [Re: cronicr]
    #20567755 - 09/14/14 06:44 PM (10 years, 4 months ago)

Quote:

Pastywhyte said:
No you pre pour the slant then PC with the lids on loose. Then pull outta the PC hot and in the SAB remove from the jar or whatever you PC'd them in and let cool on a angle. Its easier than it sounds.




Really? That sounds pretty simple.

Quote:

cronicr said:
:whathesaid: just remember to eventually use them or it becomes a habbit worse then baseball and hockey cards as a kid:lol:




Looks like there will be some test tubes in my future. I suppose I'll try making some slants when I eventually grow this PE out. If I get some nice albino fruits I'll definitely want to clone one and keep it around. Man this site is fucking great. I really didn't expect everyone to be so supportive and encouraging. I can't believe how much I've learned in the short time I've spent here. I'm so excited to see some good results. I definitely need to make a much larger SAB pretty soon :smile:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisiblePastywhyteMDiscord
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Re: About to start working with agar. [Re: NumeroEno]
    #20567806 - 09/14/14 06:51 PM (10 years, 4 months ago)

Yep its pretty damn easy. People always blow agar outta proportion in their heads and use that as the excuse not to use it. I like the screwtop slants like this one best.


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InvisibleNumeroEno
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Re: About to start working with agar. [Re: Pastywhyte]
    #20567973 - 09/14/14 07:20 PM (10 years, 4 months ago)

Hell yeah. Looking at that pic makes me want to get into edibles/medicinals as well. Having just set foot in the wonderful world of agar I have a way to go, but I think that once I have a few PE grows under my belt and have made some good isolates I'll get into slants, woodlovers, and edibles. Next year I hope to do some outdoor azurescens and morel beds and maybe do a sclerotia producing species.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
InvisibleNumeroEno
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Re: About to start working with agar. [Re: NumeroEno]
    #20595416 - 09/21/14 12:20 AM (10 years, 3 months ago)

So almost a week later I'm starting to wonder if I fucked something up. My 4 pasty plates have just been sitting on the shelf. 3 of them look like growth is starting because I see little pinpoints of white on them, but one of them shows no growth at all.

Since tomorrow morning will be the one week mark I'm wondering if I did something wrong. I'm inclined to think I didn't use enough corn syrup. I think it was pasty that told me that spores germinate marginally quicker on agar, but I've also heard PE takes longer in general.

I'm somewhat inclined to scrap the 3 of my plates that show the least growth and starting them over, letting the 4th one just chill to see what happens. Is it worth it to start over and try again with more corn syrup, or should I give all of them a few more days?

I also think I might have botched the recipe since I was drinking when I cooked the agar up. I still have plenty of material left. My recipe was 2g of agar agar, a heaping teaspoon of potato flakes, 100ml of water, and about 1/4 tsp of light corn syrup, and a few drops of blue food coloring. There's still lots of condensation in my plates as well. Which should have been gone after a few days from everything I've read.

Would someone be kind enough to clue me in on a few things?
1. Did I fuck up the recipe?
2. Is a week with very little growth out of the ordinary?

Thanks a bunch!


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisibleMudaFuka
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Re: About to start working with agar. [Re: NumeroEno]
    #20595957 - 09/21/14 06:37 AM (10 years, 3 months ago)

You  used a bit more poyato flake and and corn syrup than i would with a batch that small but it should still work fine. Dont scrap anthing just yet.  Nature doesn't have a set schedule.  Some times it can take longer than expected for spores to germinate.  You should start another set of plates while you wait. Just incase they turn out to be fucked.


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AMU
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Offlinekmetric
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Re: About to start working with agar. [Re: MudaFuka]
    #20596046 - 09/21/14 07:39 AM (10 years, 3 months ago)

If you see growth why would you scrap those? I would wait it out a bit longer..

Muda's right, make some more plates while you wait. I just started with agar as well and it took me a few dozen plates until I got good growth on a few..then those few transferred over to more, etc etc. 4 plates is very little! Keep trying.

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Offlinepawnzy
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Re: About to start working with agar. [Re: kmetric]
    #20596156 - 09/21/14 08:29 AM (10 years, 3 months ago)

Contam just is a part of life, make tons of plates and it will pay off,
just made these yesterday and just made my last 8 tranfers from mazatapec isolates

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InvisibleMudaFuka
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Re: About to start working with agar. [Re: pawnzy]
    #20596202 - 09/21/14 08:43 AM (10 years, 3 months ago)

I generally make between 20 and 30 plates in each batch. I like to take multiple transfers from each plate just in case some of them contaminate. I usually wind up throwing out a lot of clean plates in the end but I like the piece of mind.


--------------------
AMU
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