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Offlinetripdawg420
low life with no life
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Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 10 hours, 30 minutes
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Re: About to start working with agar. [Re: Mdahmer]
    #20563114 - 09/13/14 06:54 PM (10 years, 4 months ago)

or g2g to 1 spawn bag :thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge Flag
Re: About to start working with agar. [Re: tripdawg420]
    #20563135 - 09/13/14 07:03 PM (10 years, 4 months ago)

Yeah they were out of the bigger tub that I wanted. Lowes carries a 30 gallon tub that I eventually want to get for an SAB


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:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisibleMudaFuka
Poppin bottles
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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OG Cultivator
Re: About to start working with agar. [Re: NumeroEno]
    #20563137 - 09/13/14 07:04 PM (10 years, 4 months ago)

Honey loses most of its anti microbial properties after being exposed to high temperatures like the ones produced during the PC cycle. I used it for a while before I found dextrose and it works fine.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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InvisibleNumeroEno
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Registered: 07/24/14
Posts: 9,652
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Re: About to start working with agar. [Re: NumeroEno]
    #20563138 - 09/13/14 07:04 PM (10 years, 4 months ago)

My WBS just hit the 90 minute mark. As soon as I can open up my PC I'm gonna start mixing up some agar :smile:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: About to start working with agar. [Re: NumeroEno]
    #20563149 - 09/13/14 07:07 PM (10 years, 4 months ago)

Oh and here are the lids I made with polyfill. These should work, right?



--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisibleMudaFuka
Poppin bottles
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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Re: About to start working with agar. [Re: NumeroEno]
    #20563154 - 09/13/14 07:08 PM (10 years, 4 months ago)

Yah. Those should work.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 10 hours, 30 minutes
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Re: About to start working with agar. [Re: MudaFuka]
    #20563164 - 09/13/14 07:11 PM (10 years, 4 months ago)

:thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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InvisibleNumeroEno
I come from the land of lizards
Male User Gallery


Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge Flag
Re: About to start working with agar. [Re: MudaFuka]
    #20563169 - 09/13/14 07:13 PM (10 years, 4 months ago)

Cool. My WBS just finished up so I'm gonna start mixing agar. I know it's 2g agar:100ml water, but I'm housesitting right now and my scale is at my place. What's a good ratio for agar:water:potato flakes:corn syrup? Pasty's tek doesn't go into that much detail. All I have is measuring spoons, but I can get my scale if necessary.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Edited by NumeroEno (09/13/14 07:15 PM)

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InvisibleNumeroEno
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Registered: 07/24/14
Posts: 9,652
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Re: About to start working with agar. [Re: NumeroEno]
    #20563199 - 09/13/14 07:23 PM (10 years, 4 months ago)

Fuck it. Gonna get my scale. Thankfully my place is only 10 mins away on a bicycle.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
InvisibleNumeroEno
I come from the land of lizards
Male User Gallery


Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge Flag
Re: About to start working with agar. [Re: NumeroEno]
    #20563383 - 09/13/14 08:07 PM (10 years, 4 months ago)

Mixing it up now. Man I feel like an idiot because the info was there all along. Still, using my scale brought me peace of mind. Agar is on the stove right now. Decided to dye it blue because it just looked so fuckin cool.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
InvisibleNumeroEno
I come from the land of lizards
Male User Gallery


Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge Flag
Re: About to start working with agar. [Re: NumeroEno]
    #20563700 - 09/13/14 09:15 PM (10 years, 4 months ago)

Well the agar is in the PC. Wish me luck!


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,972
Loc: Canada Flag
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Re: About to start working with agar. [Re: NumeroEno]
    #20563944 - 09/13/14 10:33 PM (10 years, 4 months ago)

:goodluck: :wink:

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Offlinepawnzy
That Guy


Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
Re: About to start working with agar. [Re: Pastywhyte]
    #20563992 - 09/13/14 10:49 PM (10 years, 4 months ago)

good luck dude!

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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: About to start working with agar. [Re: pawnzy]
    #20566826 - 09/14/14 02:58 PM (10 years, 4 months ago)

Well, I inoculated 2 plates of PE uncut this morning. Think I did everything right. Do spores germinate any quicker on agar or is visible growth a week out?


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,972
Loc: Canada Flag
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Re: About to start working with agar. [Re: NumeroEno]
    #20566839 - 09/14/14 03:00 PM (10 years, 4 months ago)

Spores usually germ quicker on agar IME. Its a perfect environment for germination of anything. But like any sterile media germination is highly dependent on the spores and how old they are, genetics, etc.

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InvisibleNumeroEno
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Registered: 07/24/14
Posts: 9,652
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Re: About to start working with agar. [Re: Pastywhyte]
    #20566879 - 09/14/14 03:11 PM (10 years, 4 months ago)

Neat. After reading more I think my agar is a little deep. I filled up the plates to about 3/8" deep. As long as everything is sterile this won't be a problem, right? It will just take longer to colonize? I have a tub in it's spawn run and another 6qts of WBS colonizing right now, so I'm in no hurry to do anything with the PE. I was thinking of making liquid inoculant with one plate and storing the other one long term. How long will a colonized agar plate keep in the fridge, anyway?


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,972
Loc: Canada Flag
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Re: About to start working with agar. [Re: NumeroEno]
    #20566918 - 09/14/14 03:19 PM (10 years, 4 months ago)

If the inoculate or transferred wedges are clean it won't be a problem. It will make it very hard to isolate from bacteria tho. If you put it in a ziploc bag it can keep in the fridge for a few months maybe more.

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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: About to start working with agar. [Re: Pastywhyte]
    #20566959 - 09/14/14 03:27 PM (10 years, 4 months ago)

Well, let's just hope I did everything with the SAB right. I misted the inside with soap and water, sanitized my hands and arms, sanitized my gloves, wore tyvek sleeves, wiped the outside of the plates down with alcohol, and used a fresh sterile needle, only opening the lids enough to stick the needle in. As long as my syringe was free of bacteria everything should hopefully be fine I think. I didn't see any need to flame a needle in sterile packaging that I opened inside the SAB. Hope that wasn't a mistake.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,972
Loc: Canada Flag
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Re: About to start working with agar. [Re: NumeroEno]
    #20566997 - 09/14/14 03:38 PM (10 years, 4 months ago)

Should be fine :super:

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InvisibleMudaFuka
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Re: About to start working with agar. [Re: NumeroEno]
    #20567045 - 09/14/14 03:53 PM (10 years, 4 months ago)

I have used plates after a few months of storage in the fridge and they showed strong growth right away. I think the fact that you poured your plates a bit thick should help them to store longer without drying out.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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Shop: OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   Bridgetown Botanicals CBD Concentrates   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag


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