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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 10 hours, 31 minutes
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Re: About to start working with agar. [Re: Mdahmer]
#20563114 - 09/13/14 06:54 PM (10 years, 4 months ago) |
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or g2g to 1 spawn bag
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: tripdawg420]
#20563135 - 09/13/14 07:03 PM (10 years, 4 months ago) |
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Yeah they were out of the bigger tub that I wanted. Lowes carries a 30 gallon tub that I eventually want to get for an SAB
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: About to start working with agar. [Re: NumeroEno]
#20563137 - 09/13/14 07:04 PM (10 years, 4 months ago) |
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Honey loses most of its anti microbial properties after being exposed to high temperatures like the ones produced during the PC cycle. I used it for a while before I found dextrose and it works fine.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: NumeroEno]
#20563138 - 09/13/14 07:04 PM (10 years, 4 months ago) |
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My WBS just hit the 90 minute mark. As soon as I can open up my PC I'm gonna start mixing up some agar
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: NumeroEno]
#20563149 - 09/13/14 07:07 PM (10 years, 4 months ago) |
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Oh and here are the lids I made with polyfill. These should work, right?
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: About to start working with agar. [Re: NumeroEno]
#20563154 - 09/13/14 07:08 PM (10 years, 4 months ago) |
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Yah. Those should work.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 10 hours, 31 minutes
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Re: About to start working with agar. [Re: MudaFuka]
#20563164 - 09/13/14 07:11 PM (10 years, 4 months ago) |
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: MudaFuka]
#20563169 - 09/13/14 07:13 PM (10 years, 4 months ago) |
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Cool. My WBS just finished up so I'm gonna start mixing agar. I know it's 2g agar:100ml water, but I'm housesitting right now and my scale is at my place. What's a good ratio for agar:water:potato flakes:corn syrup? Pasty's tek doesn't go into that much detail. All I have is measuring spoons, but I can get my scale if necessary.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
Edited by NumeroEno (09/13/14 07:15 PM)
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: NumeroEno]
#20563199 - 09/13/14 07:23 PM (10 years, 4 months ago) |
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Fuck it. Gonna get my scale. Thankfully my place is only 10 mins away on a bicycle.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: NumeroEno]
#20563383 - 09/13/14 08:07 PM (10 years, 4 months ago) |
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Mixing it up now. Man I feel like an idiot because the info was there all along. Still, using my scale brought me peace of mind. Agar is on the stove right now. Decided to dye it blue because it just looked so fuckin cool.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: NumeroEno]
#20563700 - 09/13/14 09:15 PM (10 years, 4 months ago) |
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Well the agar is in the PC. Wish me luck!
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: About to start working with agar. [Re: NumeroEno]
#20563944 - 09/13/14 10:33 PM (10 years, 4 months ago) |
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pawnzy
That Guy


Registered: 03/04/14
Posts: 1,030
Last seen: 7 years, 9 months
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Re: About to start working with agar. [Re: Pastywhyte]
#20563992 - 09/13/14 10:49 PM (10 years, 4 months ago) |
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good luck dude!
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: pawnzy]
#20566826 - 09/14/14 02:58 PM (10 years, 4 months ago) |
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Well, I inoculated 2 plates of PE uncut this morning. Think I did everything right. Do spores germinate any quicker on agar or is visible growth a week out?
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: About to start working with agar. [Re: NumeroEno]
#20566839 - 09/14/14 03:00 PM (10 years, 4 months ago) |
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Spores usually germ quicker on agar IME. Its a perfect environment for germination of anything. But like any sterile media germination is highly dependent on the spores and how old they are, genetics, etc.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: Pastywhyte]
#20566879 - 09/14/14 03:11 PM (10 years, 4 months ago) |
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Neat. After reading more I think my agar is a little deep. I filled up the plates to about 3/8" deep. As long as everything is sterile this won't be a problem, right? It will just take longer to colonize? I have a tub in it's spawn run and another 6qts of WBS colonizing right now, so I'm in no hurry to do anything with the PE. I was thinking of making liquid inoculant with one plate and storing the other one long term. How long will a colonized agar plate keep in the fridge, anyway?
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: About to start working with agar. [Re: NumeroEno]
#20566918 - 09/14/14 03:19 PM (10 years, 4 months ago) |
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If the inoculate or transferred wedges are clean it won't be a problem. It will make it very hard to isolate from bacteria tho. If you put it in a ziploc bag it can keep in the fridge for a few months maybe more.
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge
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Re: About to start working with agar. [Re: Pastywhyte]
#20566959 - 09/14/14 03:27 PM (10 years, 4 months ago) |
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Well, let's just hope I did everything with the SAB right. I misted the inside with soap and water, sanitized my hands and arms, sanitized my gloves, wore tyvek sleeves, wiped the outside of the plates down with alcohol, and used a fresh sterile needle, only opening the lids enough to stick the needle in. As long as my syringe was free of bacteria everything should hopefully be fine I think. I didn't see any need to flame a needle in sterile packaging that I opened inside the SAB. Hope that wasn't a mistake.
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Let it grow! Let it grow! Greatly yield!
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in
DOG FOOD AGAR
MY ELECTRIC INOCULATION LOOP
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: About to start working with agar. [Re: NumeroEno]
#20566997 - 09/14/14 03:38 PM (10 years, 4 months ago) |
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Should be fine
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: About to start working with agar. [Re: NumeroEno]
#20567045 - 09/14/14 03:53 PM (10 years, 4 months ago) |
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I have used plates after a few months of storage in the fridge and they showed strong growth right away. I think the fact that you poured your plates a bit thick should help them to store longer without drying out.
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