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Offlinemotherchimp
Enthusiast


Registered: 03/29/14
Posts: 462
Loc: Australia Flag
Last seen: 1 year, 8 months
Moving a colonising BRF cake?
    #20559886 - 09/12/14 08:54 PM (10 years, 4 months ago)

I have a bad set of spores and theres only maybe 1/8 inch of growth between 3 half pint jars with 4 injection points. Is there a way I can maybe empty all 3 half pint jars into a plastic container and let them colonise?

I was thinking of:
1.Open the jars and scrape off the dry vermiculite layer in a SAB
2.Mix all 3 jars into a plastic container
3.Top with a layer of baked vermiculite for 10 minutes in the oven
4.Put the lid on the container and leave alone

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OfflineMonkeyJesusFresco
am i suspended in agar?
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Registered: 10/09/12
Posts: 3,308
Loc: South East USA Flag
Last seen: 24 days, 7 hours
Re: Moving a colonising BRF cake? [Re: motherchimp]
    #20559960 - 09/12/14 09:13 PM (10 years, 4 months ago)

no. that's a :failboat:

but if ya wanna try it, post pics and keep us updated :super:

edit: don't even try it, all steps involve directly introducing contaminating spores/bacterias/etc. to your substrate

Edited by MonkeyJesusFresco (09/12/14 09:15 PM)

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OfflineZytan
Stranger

Registered: 05/27/14
Posts: 218
Last seen: 7 years, 5 months
Re: Moving a colonising BRF cake? [Re: MonkeyJesusFresco]
    #20560014 - 09/12/14 09:27 PM (10 years, 4 months ago)

You went through the whole process of sterilizing your BRF jars, following all the sterile procedures during inoculation, and now you want to OPEN those jars completely exposing that mycellium to all the contaminants you were trying to avoid?

A still air box isn't a germ free box. It simply helps. Almost certainly that would ruin any progress you already made. This hobby isn't for the impatient. It took me two failures and 3 months time to see the first fruits of my work. Just set em on a shelf away from your field of vision and forget about them for 2 or 3 weeks. There is nothing you can do at this point besides disturb their progress.

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OfflineMonkeyJesusFresco
am i suspended in agar?
Male


Registered: 10/09/12
Posts: 3,308
Loc: South East USA Flag
Last seen: 24 days, 7 hours
Re: Moving a colonising BRF cake? [Re: Zytan]
    #20560104 - 09/12/14 10:01 PM (10 years, 4 months ago)

Quote:

I have a bad set of spores...




huh?

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Offlinemotherchimp
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Registered: 03/29/14
Posts: 462
Loc: Australia Flag
Last seen: 1 year, 8 months
Re: Moving a colonising BRF cake? [Re: MonkeyJesusFresco]
    #20560197 - 09/12/14 10:32 PM (10 years, 4 months ago)

Well I put spores onto agar and everywhere where I put spores turned turned a milky cream colour so some bacteria however there are about 5 spores with growth away from these.

I have 5 jars inoculated with these spores from a spore syringe and 2/5 of them have tiny amounts of growth and no contaminations so somehow there are still living spores there just very very low numbers.

Edited by motherchimp (09/12/14 11:17 PM)

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Offlinemotherchimp
Enthusiast


Registered: 03/29/14
Posts: 462
Loc: Australia Flag
Last seen: 1 year, 8 months
Re: Moving a colonising BRF cake? [Re: motherchimp]
    #20586088 - 09/18/14 10:00 PM (10 years, 3 months ago)

OK well after about 5 days after transfer I have gotten lots of growth but lots of contaminants. Heres some photos. Time to dump this in the garden before it gets any worse.




When I opened this up over half of the top dry vermiculite layer was covered in cobweb mold.

Edited by motherchimp (09/18/14 10:29 PM)

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