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Offlineschorts
a lot more and alittle less.

Registered: 09/28/03
Posts: 54
Loc: (roughly) portland, OR
Last seen: 17 years, 11 months
on the third day of PF cake...
    #2048696 - 10/27/03 08:54 PM (20 years, 5 months ago)

my true love came to me... (some friggin colonization!)

so a foaf just innoculated 12 PF cakes +little bit of crushed TVP, six with Z strain that he made into a syringe himself and dosent expect much success from, and six with orissa indian that another foaf sent him... 64 hours at last check and seeing no signs of colonization even tho it was 4 points around the edge and he saw spore water go in.

from what I racall, most people are seeing at least a little white by three days... and i'm not getting any yet.

the thermometer in the incubator has also been a little bit high, and he's been bringing it down... but it started out (after 8 hours) at about 86 F. kindof caught him by surprise because the room temp is 64 F, and he was expecting about 80... however he put his extra polyfill on top of the jars and its been holding in the heat really well. (he'd only been able to get 15 degrees over room temp with an empty box.)

anyway, should I shoot for 82 until i get colonization? should I do anythng spectacularly different? should I shut up and wait? :wink: 

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: on the third day of PF cake... [Re: schorts]
    #2048765 - 10/27/03 09:15 PM (20 years, 5 months ago)

Keep your jars as close to 85F as possible and wait...


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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Offlineschorts
a lot more and alittle less.

Registered: 09/28/03
Posts: 54
Loc: (roughly) portland, OR
Last seen: 17 years, 11 months
Re: on the third day of PF cake... [Re: dog]
    #2048779 - 10/27/03 09:18 PM (20 years, 5 months ago)

hmmm.. that shouldn't be a problem. I think theve had about the best temperature stability i could ever expect. the house stays cold all the time and the incubator stays as toasty as i want it as long as i keep my polyfill insulation in there... i've found that when i do that, the temp on the in- tub thermometer stays very consistently 2 degrees lower than the setting on the water heater.

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