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InvisibleSporulator
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Pluteus salicinus - cloned for the first time
    #20453150 - 08/21/14 11:22 AM (2 years, 10 months ago)

For decades mushroom enthusiasts are trying to grow Pluteus salicinus; without any success so far.

On July 17 I found a single specimen of Pluteus salicinus growing on a rotten log of Fagus sylvatica.

I transferred thirty tiny mushroom tissues to thirty different agar media.
Only the tissue on medium No.17 (oat+ agar) started to produce mycelium.
First I thought: "Well, probably just another contamination, like so many times before."
But after a few minutes, the damaged mycelium on the agar started to bruise.

The mycelium of Pluteus salicinus is very fragile and the hyphae are thin.
The odor is completely different compared to the mycelium of  Psilocybe and Panaeolus species

To my knowledge, this is the first report of a cloned Pluteus salicinus specimen.

I name this strain of Pluteus salicinus  SWISS-2014/7  due to its origin

This specimen of Pluteus salicinus was found in Europe. As I learned from Alan and Gravija,  Pluteus salicinus does not occur in North America.
All bluing Pluteus specimens found in North America are different or undescribed species.






Pluteus salicinus

                                   




Habitat







Pluteus salicinus mycelium on PDA, intact and damaged:

             





The original clone on agar:







First cultivation attempts, the dark substrate consists of oat flakes and sawdust from a very rotten log:

                                     


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Edited by Sporulator (08/21/14 02:48 PM)


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OfflineAnglerfish
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Re: Pluteus salicinus - cloned for the first time [Re: Sporulator]
    #20453291 - 08/21/14 12:13 PM (2 years, 10 months ago)

Excellent! Is it fast growing? How will you go about trying to fruit it - if it is possible?


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InvisibleSporulator
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Re: Pluteus salicinus - cloned for the first time [Re: Anglerfish]
    #20453332 - 08/21/14 12:25 PM (2 years, 10 months ago)

Quote:

Anglerfish said:
Excellent! Is it fast growing? How will you go about trying to fruit it - if it is possible?




It grows faster than the grassland Psilocybes (Ps. stuntzii, hispanica, semilanceata etc.) but a little bit slower than the woodlover Psilocybes.

In my country, I find Pluteus salicinus always near ponds and creeks. A high relative air humidity seems to be very important.
In Europe,  Pluteus salicinus fruits from May to October, so no cold shock will be necessary I suppose.
I will give it 90%-100%  air humidity and at least three air exchanges/hour.


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OfflineAlan RockefellerM
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Re: Pluteus salicinus - cloned for the first time [Re: Sporulator]
    #20453342 - 08/21/14 12:28 PM (2 years, 10 months ago)

Wow this is cool!    It does look a bit different than the species that we call P. salicinus in North America.


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OfflineAnglerfish
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Re: Pluteus salicinus - cloned for the first time [Re: Sporulator]
    #20453382 - 08/21/14 12:34 PM (2 years, 10 months ago)

Quote:

Sporulator said:
In my country, I find Pluteus salicinus always near ponds and creeks. A high relative air humidity seems to be very important.
In Europe,  Pluteus salicinus fruits from May to October, so no cold shock will be necessary I suppose.
I will give it 90%-100%  air humidity and at least three air exchanges/hour.




Will you try to spawn it to a rotten log, or perhaps try to replicate one?

I'm wondering how the colonization process with this species works in nature - and what
ignites the fruiting process.


--------------------


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InvisibleSporulator
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Re: Pluteus salicinus - cloned for the first time [Re: Alan Rockefeller]
    #20453399 - 08/21/14 12:37 PM (2 years, 10 months ago)

Quote:

Alan Rockefeller said:
Wow this is cool!    It does look a bit different than the species that we call P. salicinus in North America.




Thanks!  It seems that there is a lot of confusion concerning the bluing Pluteus species...  :confused:


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InvisibleSporulator
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Re: Pluteus salicinus - cloned for the first time [Re: Anglerfish]
    #20453419 - 08/21/14 12:43 PM (2 years, 10 months ago)

Quote:

Anglerfish said:
Quote:

Sporulator said:
In my country, I find Pluteus salicinus always near ponds and creeks. A high relative air humidity seems to be very important.
In Europe,  Pluteus salicinus fruits from May to October, so no cold shock will be necessary I suppose.
I will give it 90%-100%  air humidity and at least three air exchanges/hour.




Will you try to spawn it to a rotten log, or perhaps try to replicate one?

I'm wondering how the colonization process with this species works in nature - and what
ignites the fruiting process.




      First I will try to fruit them indoors in jars.
      This is completely uncharted territory and first I have to work out all the parameters by trial and error.


--------------------
                 


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Invisible36fuckin5
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Re: Pluteus salicinus - cloned for the first time [Re: Sporulator]
    #20453421 - 08/21/14 12:44 PM (2 years, 10 months ago)

Nice! Hopefully you can get it to fruit!


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OfflineAlan RockefellerM
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Re: Pluteus salicinus - cloned for the first time [Re: Sporulator] * 1
    #20453440 - 08/21/14 12:47 PM (2 years, 10 months ago)

Also consider making some plug spawn.  I do this in small batches by tossing wet dowel rods on top of a colonized petri dish.


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InvisibleSporulator
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Re: Pluteus salicinus - cloned for the first time *DELETED* [Re: Alan Rockefeller]
    #20453465 - 08/21/14 12:53 PM (2 years, 10 months ago)

Post deleted by Sporulator

Reason for deletion: .



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Offlineknarkkorven
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Re: Pluteus salicinus - cloned for the first time [Re: Sporulator]
    #20453515 - 08/21/14 01:04 PM (2 years, 10 months ago)

Wow! Very nice! It looks great! :super:

This is a big step in the even harder process to produce fruit bodies. I wish you good luck with that!


Edited by knarkkorven (08/21/14 01:15 PM)


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InvisibleSporulator
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Re: Pluteus salicinus - cloned for the first time [Re: Alan Rockefeller]
    #20453527 - 08/21/14 01:08 PM (2 years, 10 months ago)

Quote:

36fuckin5 said:
Nice! Hopefully you can get it to fruit!




      Thanks, let's hope for the best!


Quote:

Alan Rockefeller said:
Also consider making some plug spawn.  I do this in small batches by tossing wet dowel rods on top of a colonized petri dish.




      Yes, dowels always work fine, because they don't contaminate.


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InvisibleSporulator
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Re: Pluteus salicinus - cloned for the first time [Re: knarkkorven]
    #20453539 - 08/21/14 01:12 PM (2 years, 10 months ago)

Quote:

knarkkorven said:
Wow! Very nice! It looks great! :super:

This is a big step in the even harder process to produce fruit bodies. I wish you good luck with that!




      Thanks!  Let's hope that to fruit them is much easier than to clone them or to germinate the spores.


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Offlineknarkkorven
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Re: Pluteus salicinus - cloned for the first time [Re: Sporulator]
    #20453561 - 08/21/14 01:18 PM (2 years, 10 months ago)

Just find some good, old partly decomposed wood like those logs you picked it from. Birch is also supposed to be good.


I have found P. salicinus once, and I've been hunting many times since then in perfects habitats without success.. They are very hard to come by!

Like you say, there seems to be a lot of confusion regarding the species and the potency. Remember that Gartz found >1% psilocybin in samples from Germany. Europe might have a really potent species out there.


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InvisibleByrain

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Re: Pluteus salicinus - cloned for the first time [Re: Sporulator]
    #20453564 - 08/21/14 01:19 PM (2 years, 10 months ago)

What makes this P. salicinus and not one of the other active Pluteus spp.?


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Offlineknarkkorven
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Re: Pluteus salicinus - cloned for the first time [Re: Byrain]
    #20453585 - 08/21/14 01:25 PM (2 years, 10 months ago)

Quote:

What makes this P. salicinus and not one of the other active Pluteus spp.?




The cap color is what makes me certain.
But this is another "evidence":

Quote:

found in Europe




I don't think we have any other. The exception would be if the bluing mushrooms (psilocin) are one species and the non-bluing (psilocybin) another.


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InvisibleSporulator
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Re: Pluteus salicinus - cloned for the first time [Re: Byrain]
    #20453604 - 08/21/14 01:31 PM (2 years, 10 months ago)

Quote:

Byrain said:
What makes this P. salicinus and not one of the other active Pluteus spp.?




      1. It was found in Europe. Pluteus salicinus and Pluteus cyanopus are the only active Pluteus species in Europe.

      2. The microscopic characteristics match perfectly with those from that site:

      http://www.funghiitaliani.it/?showtopic=24371




      A nice video of a Pluteus salicinus specimen from Europe:

     




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Edited by Sporulator (08/21/14 01:53 PM)


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InvisibleSporulator
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Re: Pluteus salicinus - cloned for the first time [Re: knarkkorven]
    #20453655 - 08/21/14 01:47 PM (2 years, 10 months ago)

Quote:

knarkkorven said:

I have found P. salicinus once, and I've been hunting many times since then in perfects habitats without success.. They are very hard to come by!

Like you say, there seems to be a lot of confusion regarding the species and the potency. Remember that Gartz found >1% psilocybin in samples from Germany. Europe might have a really potent species out there.




      Yes, they are rare and hard to find and I think that they are more potent than people might think.


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InvisibleByrain

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Re: Pluteus salicinus - cloned for the first time [Re: knarkkorven]
    #20453672 - 08/21/14 01:52 PM (2 years, 10 months ago)

The fan2 Pluteus key used to be visible at google books, doesn't seem to be there anymore... :frown:

This also lists P. villosus, P. cyanopus & P. nigroviridis for Europe. :shrug:


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Offlineknarkkorven
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Re: Pluteus salicinus - cloned for the first time [Re: Byrain]
    #20453773 - 08/21/14 02:23 PM (2 years, 10 months ago)

Yeah. I forgot those... Apparently they are very rare, P. salicinus is considered common by a german source whereas cyanopus is considered very rare. And most of us think salicinus is rare.. so, that would mean cyanopus is extremely rare. :smile:

This is supported by very few documented records from Europe:
http://data.gbif.org/occurrences/search.htm?c0.s=0&c0.p=0&c0.o=Pluteus+cyanopus
http://www.discoverlife.org/mp/20m?kind=Pluteus+cyanopus

...And I don't know how correct this source is, but it's also pointing in this direction, and mentions that Pluteus nigroviridis is pretty much non-active:

Translation:
Quote:

For Europe, only one type is relevant, (described over a hundred years ago) the separation of Pluteus cyanopus (Quelet) Met-rod and pluteus nigroviridis Babos of Hungary (Gartz 1999, 2003) (only 0.035% psilocybin) are constant no data available therefore find these types no further mention.




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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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